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Pathological Comparisons of the Hippocampal Changes in the Transient and Permanent Middle Cerebral Artery Occlusion Rat Models

Ischemic strokes are categorized by permanent or transient obstruction of blood flow, which impedes delivery of oxygen and essential nutrients to brain. In the last decade, the therapeutic window for tPA has increased from 3 to 5–6 h, and a new technique, involving the mechanical removal of the clot...

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Autores principales: Shah, Fawad Ali, Li, Tao, Kury, Lina Tariq Al, Zeb, Alam, Khatoon, Shehla, Liu, Gongping, Yang, Xifei, Liu, Fang, Yao, Huo, Khan, Arif-Ullah, Koh, Phil Ok, Jiang, Yuhua, Li, Shupeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6868119/
https://www.ncbi.nlm.nih.gov/pubmed/31798514
http://dx.doi.org/10.3389/fneur.2019.01178
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author Shah, Fawad Ali
Li, Tao
Kury, Lina Tariq Al
Zeb, Alam
Khatoon, Shehla
Liu, Gongping
Yang, Xifei
Liu, Fang
Yao, Huo
Khan, Arif-Ullah
Koh, Phil Ok
Jiang, Yuhua
Li, Shupeng
author_facet Shah, Fawad Ali
Li, Tao
Kury, Lina Tariq Al
Zeb, Alam
Khatoon, Shehla
Liu, Gongping
Yang, Xifei
Liu, Fang
Yao, Huo
Khan, Arif-Ullah
Koh, Phil Ok
Jiang, Yuhua
Li, Shupeng
author_sort Shah, Fawad Ali
collection PubMed
description Ischemic strokes are categorized by permanent or transient obstruction of blood flow, which impedes delivery of oxygen and essential nutrients to brain. In the last decade, the therapeutic window for tPA has increased from 3 to 5–6 h, and a new technique, involving the mechanical removal of the clot (endovascular thrombectomy) to allow reperfusion of the injured area, is being used more often. This last therapeutic approach can be done until 24 h after stroke onset. Due to this fact, more acute ischemic stroke patients are now being recanalized, and so tMCAO is probably the “best” model to address these patients that have a potential good outcome in terms of survival and functional recovery. However, permanent occlusion patients are also important, not only to increase survival rate but also to improve functional outcomes, although these are more difficult to achieve. So, both models are important, and which target different stroke patients in the clinical scenario. Hippocampus has a vital role in memory and cognition, is prone to ischemic induced neurodegeneration. This study was designed to delineate the molecular, pathological, and neurological changes in rat models of t-MCAO, permanent MCAO (pMCAO), and pMCAO with diabetic conditions in hippocampal tissue. Our results showed that these three models showed distinct discrepancies at numerous pathological process, including key signaling molecules involved in neuronal apoptosis, glutamate induced excitotoxicity, neuroinflammation, oxidative stress, and neurotrophic changes. Our result suggests that the two commonly used MCAO models exhibited tremendous differences in terms of neuronal cell loss, glutamate excitotoxic related signaling, synaptic transmission markers, neuron inflammatory and oxidative stress molecules. These differences may reflect the variations in different models, which may provide valuable information for mechanistic and therapeutic inconsistences as experienced in both preclinical models and clinical trials.
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spelling pubmed-68681192019-12-03 Pathological Comparisons of the Hippocampal Changes in the Transient and Permanent Middle Cerebral Artery Occlusion Rat Models Shah, Fawad Ali Li, Tao Kury, Lina Tariq Al Zeb, Alam Khatoon, Shehla Liu, Gongping Yang, Xifei Liu, Fang Yao, Huo Khan, Arif-Ullah Koh, Phil Ok Jiang, Yuhua Li, Shupeng Front Neurol Neurology Ischemic strokes are categorized by permanent or transient obstruction of blood flow, which impedes delivery of oxygen and essential nutrients to brain. In the last decade, the therapeutic window for tPA has increased from 3 to 5–6 h, and a new technique, involving the mechanical removal of the clot (endovascular thrombectomy) to allow reperfusion of the injured area, is being used more often. This last therapeutic approach can be done until 24 h after stroke onset. Due to this fact, more acute ischemic stroke patients are now being recanalized, and so tMCAO is probably the “best” model to address these patients that have a potential good outcome in terms of survival and functional recovery. However, permanent occlusion patients are also important, not only to increase survival rate but also to improve functional outcomes, although these are more difficult to achieve. So, both models are important, and which target different stroke patients in the clinical scenario. Hippocampus has a vital role in memory and cognition, is prone to ischemic induced neurodegeneration. This study was designed to delineate the molecular, pathological, and neurological changes in rat models of t-MCAO, permanent MCAO (pMCAO), and pMCAO with diabetic conditions in hippocampal tissue. Our results showed that these three models showed distinct discrepancies at numerous pathological process, including key signaling molecules involved in neuronal apoptosis, glutamate induced excitotoxicity, neuroinflammation, oxidative stress, and neurotrophic changes. Our result suggests that the two commonly used MCAO models exhibited tremendous differences in terms of neuronal cell loss, glutamate excitotoxic related signaling, synaptic transmission markers, neuron inflammatory and oxidative stress molecules. These differences may reflect the variations in different models, which may provide valuable information for mechanistic and therapeutic inconsistences as experienced in both preclinical models and clinical trials. Frontiers Media S.A. 2019-11-14 /pmc/articles/PMC6868119/ /pubmed/31798514 http://dx.doi.org/10.3389/fneur.2019.01178 Text en Copyright © 2019 Shah, Li, Kury, Zeb, Khatoon, Liu, Yang, Liu, Yao, Khan, Koh, Jiang and Li. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neurology
Shah, Fawad Ali
Li, Tao
Kury, Lina Tariq Al
Zeb, Alam
Khatoon, Shehla
Liu, Gongping
Yang, Xifei
Liu, Fang
Yao, Huo
Khan, Arif-Ullah
Koh, Phil Ok
Jiang, Yuhua
Li, Shupeng
Pathological Comparisons of the Hippocampal Changes in the Transient and Permanent Middle Cerebral Artery Occlusion Rat Models
title Pathological Comparisons of the Hippocampal Changes in the Transient and Permanent Middle Cerebral Artery Occlusion Rat Models
title_full Pathological Comparisons of the Hippocampal Changes in the Transient and Permanent Middle Cerebral Artery Occlusion Rat Models
title_fullStr Pathological Comparisons of the Hippocampal Changes in the Transient and Permanent Middle Cerebral Artery Occlusion Rat Models
title_full_unstemmed Pathological Comparisons of the Hippocampal Changes in the Transient and Permanent Middle Cerebral Artery Occlusion Rat Models
title_short Pathological Comparisons of the Hippocampal Changes in the Transient and Permanent Middle Cerebral Artery Occlusion Rat Models
title_sort pathological comparisons of the hippocampal changes in the transient and permanent middle cerebral artery occlusion rat models
topic Neurology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6868119/
https://www.ncbi.nlm.nih.gov/pubmed/31798514
http://dx.doi.org/10.3389/fneur.2019.01178
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