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Mechanically rigid supramolecular assemblies formed from an Fmoc-guanine conjugated peptide nucleic acid
The variety and complexity of DNA-based structures make them attractive candidates for nanotechnology, yet insufficient stability and mechanical rigidity, compared to polyamide-based molecules, limit their application. Here, we combine the advantages of polyamide materials and the structural pattern...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6868146/ https://www.ncbi.nlm.nih.gov/pubmed/31748568 http://dx.doi.org/10.1038/s41467-019-13250-x |
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author | Basavalingappa, Vasantha Bera, Santu Xue, Bin Azuri, Ido Tang, Yiming Tao, Kai Shimon, Linda J. W. Sawaya, Michael R. Kolusheva, Sofiya Eisenberg, David S. Kronik, Leeor Cao, Yi Wei, Guanghong Gazit, Ehud |
author_facet | Basavalingappa, Vasantha Bera, Santu Xue, Bin Azuri, Ido Tang, Yiming Tao, Kai Shimon, Linda J. W. Sawaya, Michael R. Kolusheva, Sofiya Eisenberg, David S. Kronik, Leeor Cao, Yi Wei, Guanghong Gazit, Ehud |
author_sort | Basavalingappa, Vasantha |
collection | PubMed |
description | The variety and complexity of DNA-based structures make them attractive candidates for nanotechnology, yet insufficient stability and mechanical rigidity, compared to polyamide-based molecules, limit their application. Here, we combine the advantages of polyamide materials and the structural patterns inspired by nucleic-acids to generate a mechanically rigid fluorenylmethyloxycarbonyl (Fmoc)-guanine peptide nucleic acid (PNA) conjugate with diverse morphology and photoluminescent properties. The assembly possesses a unique atomic structure, with each guanine head of one molecule hydrogen bonded to the Fmoc carbonyl tail of another molecule, generating a non-planar cyclic quartet arrangement. This structure exhibits an average stiffness of 69.6 ± 6.8 N m(−1) and Young’s modulus of 17.8 ± 2.5 GPa, higher than any previously reported nucleic acid derived structure. This data suggests that the unique cation-free “basket” formed by the Fmoc-G-PNA conjugate can serve as an attractive component for the design of new materials based on PNA self-assembly for nanotechnology applications. |
format | Online Article Text |
id | pubmed-6868146 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-68681462019-11-22 Mechanically rigid supramolecular assemblies formed from an Fmoc-guanine conjugated peptide nucleic acid Basavalingappa, Vasantha Bera, Santu Xue, Bin Azuri, Ido Tang, Yiming Tao, Kai Shimon, Linda J. W. Sawaya, Michael R. Kolusheva, Sofiya Eisenberg, David S. Kronik, Leeor Cao, Yi Wei, Guanghong Gazit, Ehud Nat Commun Article The variety and complexity of DNA-based structures make them attractive candidates for nanotechnology, yet insufficient stability and mechanical rigidity, compared to polyamide-based molecules, limit their application. Here, we combine the advantages of polyamide materials and the structural patterns inspired by nucleic-acids to generate a mechanically rigid fluorenylmethyloxycarbonyl (Fmoc)-guanine peptide nucleic acid (PNA) conjugate with diverse morphology and photoluminescent properties. The assembly possesses a unique atomic structure, with each guanine head of one molecule hydrogen bonded to the Fmoc carbonyl tail of another molecule, generating a non-planar cyclic quartet arrangement. This structure exhibits an average stiffness of 69.6 ± 6.8 N m(−1) and Young’s modulus of 17.8 ± 2.5 GPa, higher than any previously reported nucleic acid derived structure. This data suggests that the unique cation-free “basket” formed by the Fmoc-G-PNA conjugate can serve as an attractive component for the design of new materials based on PNA self-assembly for nanotechnology applications. Nature Publishing Group UK 2019-11-20 /pmc/articles/PMC6868146/ /pubmed/31748568 http://dx.doi.org/10.1038/s41467-019-13250-x Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Basavalingappa, Vasantha Bera, Santu Xue, Bin Azuri, Ido Tang, Yiming Tao, Kai Shimon, Linda J. W. Sawaya, Michael R. Kolusheva, Sofiya Eisenberg, David S. Kronik, Leeor Cao, Yi Wei, Guanghong Gazit, Ehud Mechanically rigid supramolecular assemblies formed from an Fmoc-guanine conjugated peptide nucleic acid |
title | Mechanically rigid supramolecular assemblies formed from an Fmoc-guanine conjugated peptide nucleic acid |
title_full | Mechanically rigid supramolecular assemblies formed from an Fmoc-guanine conjugated peptide nucleic acid |
title_fullStr | Mechanically rigid supramolecular assemblies formed from an Fmoc-guanine conjugated peptide nucleic acid |
title_full_unstemmed | Mechanically rigid supramolecular assemblies formed from an Fmoc-guanine conjugated peptide nucleic acid |
title_short | Mechanically rigid supramolecular assemblies formed from an Fmoc-guanine conjugated peptide nucleic acid |
title_sort | mechanically rigid supramolecular assemblies formed from an fmoc-guanine conjugated peptide nucleic acid |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6868146/ https://www.ncbi.nlm.nih.gov/pubmed/31748568 http://dx.doi.org/10.1038/s41467-019-13250-x |
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