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Immune response evaluation in Balb/c mice after crude extract of Anisakis typica sensitization
BACKGROUND AND AIM: Anisakis is a global challenge for a fish product which may lead to a decrease in economic value and consumers’ preference. Skipjack (Katsuwonus pelamis) in Kupang, Nusa Tenggara Timur, Indonesia, have important economic value for local fisheries. Anisakis typica is one of the An...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Veterinary World
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6868255/ https://www.ncbi.nlm.nih.gov/pubmed/31849412 http://dx.doi.org/10.14202/vetworld.2019.1529-1534 |
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author | Haryadi, Linda Suprayitno, Eddy Aulanni’am, Aulanni’am Hariati, Anik Martinah |
author_facet | Haryadi, Linda Suprayitno, Eddy Aulanni’am, Aulanni’am Hariati, Anik Martinah |
author_sort | Haryadi, Linda |
collection | PubMed |
description | BACKGROUND AND AIM: Anisakis is a global challenge for a fish product which may lead to a decrease in economic value and consumers’ preference. Skipjack (Katsuwonus pelamis) in Kupang, Nusa Tenggara Timur, Indonesia, have important economic value for local fisheries. Anisakis typica is one of the Anisakis species which potent to induce an allergic reaction. However, the study about A. typica involved in the dendritic cells (DCs), T helper 1 (Th(1)), T helper 2 (Th(2)), and regulatory T cells (Tregs) is still limited. This study aimed to analyze the dynamic changed of the immune system including DCs, CD4(+) T cells, and Tregs after 1 week of A. typica sensitization. MATERIALS AND METHODS: Twenty-four male Balb/C mice were randomly divided into four groups (n=6), mice treated with crude A. typica extract (CAE) 50, 75, and 100 mg/kg BW, respectively. CAE was given orally per day for a week. At the end of the experiment, the animals were sacrificed and the spleen was collected. DCs were labeled as CD11c(+) interleukin-6(+) (IL-6(+)); CD4(+) T cells were distinguished as Th(1) (CD4(+) interferon-γ(+) [IFN-γ(+)]) and Th(2) (CD4(+) IL-4(+) and CD4(+) IL-5(+)); Tregs were labeled as CD4(+)CD25(+)CD62L(+). The expression of each cell was determined by flow cytometry. RESULTS: Our result described that CAE elicits CD11c(+) IL-6(+), CD4(+) IFN-γ(+), CD4(+) IL-4(+), and CD4(+) IL-5(+) and reduces CD4(+)CD25(+)CD62L(+) significantly (p<0.05) in dose-dependent manner in mice after A. typica infection. CONCLUSION: The Th(1)/Th(2) ratio after A. typica crude extract treatment exhibits a mixed pattern rather than the classical model allergy to food antigens. Our study is expected as a basic understanding of the changes in immune response after A. typic a infection. |
format | Online Article Text |
id | pubmed-6868255 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Veterinary World |
record_format | MEDLINE/PubMed |
spelling | pubmed-68682552019-12-17 Immune response evaluation in Balb/c mice after crude extract of Anisakis typica sensitization Haryadi, Linda Suprayitno, Eddy Aulanni’am, Aulanni’am Hariati, Anik Martinah Vet World Research Article BACKGROUND AND AIM: Anisakis is a global challenge for a fish product which may lead to a decrease in economic value and consumers’ preference. Skipjack (Katsuwonus pelamis) in Kupang, Nusa Tenggara Timur, Indonesia, have important economic value for local fisheries. Anisakis typica is one of the Anisakis species which potent to induce an allergic reaction. However, the study about A. typica involved in the dendritic cells (DCs), T helper 1 (Th(1)), T helper 2 (Th(2)), and regulatory T cells (Tregs) is still limited. This study aimed to analyze the dynamic changed of the immune system including DCs, CD4(+) T cells, and Tregs after 1 week of A. typica sensitization. MATERIALS AND METHODS: Twenty-four male Balb/C mice were randomly divided into four groups (n=6), mice treated with crude A. typica extract (CAE) 50, 75, and 100 mg/kg BW, respectively. CAE was given orally per day for a week. At the end of the experiment, the animals were sacrificed and the spleen was collected. DCs were labeled as CD11c(+) interleukin-6(+) (IL-6(+)); CD4(+) T cells were distinguished as Th(1) (CD4(+) interferon-γ(+) [IFN-γ(+)]) and Th(2) (CD4(+) IL-4(+) and CD4(+) IL-5(+)); Tregs were labeled as CD4(+)CD25(+)CD62L(+). The expression of each cell was determined by flow cytometry. RESULTS: Our result described that CAE elicits CD11c(+) IL-6(+), CD4(+) IFN-γ(+), CD4(+) IL-4(+), and CD4(+) IL-5(+) and reduces CD4(+)CD25(+)CD62L(+) significantly (p<0.05) in dose-dependent manner in mice after A. typica infection. CONCLUSION: The Th(1)/Th(2) ratio after A. typica crude extract treatment exhibits a mixed pattern rather than the classical model allergy to food antigens. Our study is expected as a basic understanding of the changes in immune response after A. typic a infection. Veterinary World 2019-10 2019-10-04 /pmc/articles/PMC6868255/ /pubmed/31849412 http://dx.doi.org/10.14202/vetworld.2019.1529-1534 Text en Copyright: © Haryadi, et al. http://creativecommons.org/licenses/by/4.0 Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Haryadi, Linda Suprayitno, Eddy Aulanni’am, Aulanni’am Hariati, Anik Martinah Immune response evaluation in Balb/c mice after crude extract of Anisakis typica sensitization |
title | Immune response evaluation in Balb/c mice after crude extract of Anisakis typica sensitization |
title_full | Immune response evaluation in Balb/c mice after crude extract of Anisakis typica sensitization |
title_fullStr | Immune response evaluation in Balb/c mice after crude extract of Anisakis typica sensitization |
title_full_unstemmed | Immune response evaluation in Balb/c mice after crude extract of Anisakis typica sensitization |
title_short | Immune response evaluation in Balb/c mice after crude extract of Anisakis typica sensitization |
title_sort | immune response evaluation in balb/c mice after crude extract of anisakis typica sensitization |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6868255/ https://www.ncbi.nlm.nih.gov/pubmed/31849412 http://dx.doi.org/10.14202/vetworld.2019.1529-1534 |
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