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Preliminary investigation of acaricidal activity of leaf extract of Nicotiana tabacum on dog tick Rhipicephalus sanguineus

BACKGROUND AND AIM: Tick infestation of domestic animals remains a major constraint to livestock productivity across all agro-ecological zones most especially in small animal practice. The most common method of tick control is the use of synthetic acaricide. However, a widespread increase of acarici...

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Autores principales: Oyagbemi, Taiwo Olaide, Ashafa, Anofi, Adejinmi, Johnson Olayide, Oguntibeju, Oluwafemi Omoniyi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Veterinary World 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6868261/
https://www.ncbi.nlm.nih.gov/pubmed/31849425
http://dx.doi.org/10.14202/vetworld.2019.1624-1629
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author Oyagbemi, Taiwo Olaide
Ashafa, Anofi
Adejinmi, Johnson Olayide
Oguntibeju, Oluwafemi Omoniyi
author_facet Oyagbemi, Taiwo Olaide
Ashafa, Anofi
Adejinmi, Johnson Olayide
Oguntibeju, Oluwafemi Omoniyi
author_sort Oyagbemi, Taiwo Olaide
collection PubMed
description BACKGROUND AND AIM: Tick infestation of domestic animals remains a major constraint to livestock productivity across all agro-ecological zones most especially in small animal practice. The most common method of tick control is the use of synthetic acaricide. However, a widespread increase of acaricidal resistance, scarcity and high cost of acaricides especially to farmers of low-income earnings in developing countries support the need for alternative tick control methods. Among the alternative methods for tick control is herbal therapy. In this study, we investigated the acaricidal activity of methanol and N-hexane leaf extracts of Nicotiana tabacum against dog ticks – Rhipicephalus sanguineus. MATERIALS AND METHODS: Larvicidal and adulticidal activity of N. tabacum leaf extract were examined on the dog tick – R. sanguineus in an in vitro experiment using larval packet test and adult immersion test respectively. Phytochemical and Gas Chromatography-Mass Spectrometry (GC–MS) analysis of the leaf extract were also carried out using standard methods. RESULTS: We observed a tick mortality rate that was concentration-dependent. However, N-hexane extract showed a higher significant acaricidal effect than methanol extract. Lethal dose (LD(50)) of N. tabacum was 0.06. High quantity of terpenoids was obtained from N. tabacum. Lower tick glutathione S-transferase observed with varying concentration of N. tabacum. GC–MS revealed Pyridine, 3-(1-methyl-2-pyrrolidinyl)-, (S) - Nicotine, Citronellyl propionate, Crotonaldehyde, Lavandulyl acetate, trans-Phytol and Amitrole (3-Amino-1, 2, 4-triazole) in N. tabacum. CONCLUSION: Both methanol and N-hexane leaf extracts of N. tabacum exhibited observable acaricidal property against the larvae and adult R. sanguineus of dog.
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spelling pubmed-68682612019-12-17 Preliminary investigation of acaricidal activity of leaf extract of Nicotiana tabacum on dog tick Rhipicephalus sanguineus Oyagbemi, Taiwo Olaide Ashafa, Anofi Adejinmi, Johnson Olayide Oguntibeju, Oluwafemi Omoniyi Vet World Research Article BACKGROUND AND AIM: Tick infestation of domestic animals remains a major constraint to livestock productivity across all agro-ecological zones most especially in small animal practice. The most common method of tick control is the use of synthetic acaricide. However, a widespread increase of acaricidal resistance, scarcity and high cost of acaricides especially to farmers of low-income earnings in developing countries support the need for alternative tick control methods. Among the alternative methods for tick control is herbal therapy. In this study, we investigated the acaricidal activity of methanol and N-hexane leaf extracts of Nicotiana tabacum against dog ticks – Rhipicephalus sanguineus. MATERIALS AND METHODS: Larvicidal and adulticidal activity of N. tabacum leaf extract were examined on the dog tick – R. sanguineus in an in vitro experiment using larval packet test and adult immersion test respectively. Phytochemical and Gas Chromatography-Mass Spectrometry (GC–MS) analysis of the leaf extract were also carried out using standard methods. RESULTS: We observed a tick mortality rate that was concentration-dependent. However, N-hexane extract showed a higher significant acaricidal effect than methanol extract. Lethal dose (LD(50)) of N. tabacum was 0.06. High quantity of terpenoids was obtained from N. tabacum. Lower tick glutathione S-transferase observed with varying concentration of N. tabacum. GC–MS revealed Pyridine, 3-(1-methyl-2-pyrrolidinyl)-, (S) - Nicotine, Citronellyl propionate, Crotonaldehyde, Lavandulyl acetate, trans-Phytol and Amitrole (3-Amino-1, 2, 4-triazole) in N. tabacum. CONCLUSION: Both methanol and N-hexane leaf extracts of N. tabacum exhibited observable acaricidal property against the larvae and adult R. sanguineus of dog. Veterinary World 2019-10 2019-10-25 /pmc/articles/PMC6868261/ /pubmed/31849425 http://dx.doi.org/10.14202/vetworld.2019.1624-1629 Text en Copyright: © Oyagbemi, et al. http://creativecommons.org/licenses/by/4.0 Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Oyagbemi, Taiwo Olaide
Ashafa, Anofi
Adejinmi, Johnson Olayide
Oguntibeju, Oluwafemi Omoniyi
Preliminary investigation of acaricidal activity of leaf extract of Nicotiana tabacum on dog tick Rhipicephalus sanguineus
title Preliminary investigation of acaricidal activity of leaf extract of Nicotiana tabacum on dog tick Rhipicephalus sanguineus
title_full Preliminary investigation of acaricidal activity of leaf extract of Nicotiana tabacum on dog tick Rhipicephalus sanguineus
title_fullStr Preliminary investigation of acaricidal activity of leaf extract of Nicotiana tabacum on dog tick Rhipicephalus sanguineus
title_full_unstemmed Preliminary investigation of acaricidal activity of leaf extract of Nicotiana tabacum on dog tick Rhipicephalus sanguineus
title_short Preliminary investigation of acaricidal activity of leaf extract of Nicotiana tabacum on dog tick Rhipicephalus sanguineus
title_sort preliminary investigation of acaricidal activity of leaf extract of nicotiana tabacum on dog tick rhipicephalus sanguineus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6868261/
https://www.ncbi.nlm.nih.gov/pubmed/31849425
http://dx.doi.org/10.14202/vetworld.2019.1624-1629
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