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Immunization with recombinant enolase of Sporothrix spp. (rSsEno) confers effective protection against sporotrichosis in mice
In recent years, research has focused on the immunoreactive components of the Sporothrix schenckii cell wall that can be relevant targets for preventive and therapeutic vaccines against sporotrichosis, an emergent worldwide mycosis. In a previous study, we identified a 47-kDa enolase as an immunodom...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6868355/ https://www.ncbi.nlm.nih.gov/pubmed/31748544 http://dx.doi.org/10.1038/s41598-019-53135-z |
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author | Portuondo, Deivys Leandro Dores-Silva, Paulo Roberto Ferreira, Lucas Souza de Oliveira, Carlos S. Téllez-Martínez, Damiana Marcos, Caroline Maria de Aguiar Loesch, Maria Luiza Guzmán, Fanny Gava, Lisandra M. Borges, Júlio César Pereira, Sandro Antonio Batista-Duharte, Alexander Carlos, Iracilda Zeppone |
author_facet | Portuondo, Deivys Leandro Dores-Silva, Paulo Roberto Ferreira, Lucas Souza de Oliveira, Carlos S. Téllez-Martínez, Damiana Marcos, Caroline Maria de Aguiar Loesch, Maria Luiza Guzmán, Fanny Gava, Lisandra M. Borges, Júlio César Pereira, Sandro Antonio Batista-Duharte, Alexander Carlos, Iracilda Zeppone |
author_sort | Portuondo, Deivys Leandro |
collection | PubMed |
description | In recent years, research has focused on the immunoreactive components of the Sporothrix schenckii cell wall that can be relevant targets for preventive and therapeutic vaccines against sporotrichosis, an emergent worldwide mycosis. In a previous study, we identified a 47-kDa enolase as an immunodominant antigen in mice vaccinated with an adjuvanted mixture of S. schenckii cell wall proteins. Here, we sought to assess the protective potential of a Sporothrix spp. recombinant enolase (rSsEno) formulated with or without the adjuvant Montanide Pet-GelA (PGA) against the S. brasiliensis infection in mice. Mice that were immunized with rSsEno plus PGA showed increased antibody titters against rSsEno and increased median survival time when challenged with S. brasiliensis as compared with mice that had not been immunized or that were immunized with rSsEno alone. Immunization with rSsEno plus PGA induced a predominantly T-helper 1 cytokine pattern after in vitro stimulation of splenic cells with rSsEno: elevated levels of IFN-γ and IL-2, as well as of other cytokines involved in host defense against sporotrichosis, such as TNF-alpha, IL-6, and IL-4. Furthermore, we show for the first time the presence of enolase in the cell wall of both S. schenckii and S. brasiliensis. As a whole, our results suggest that enolase could be used as a potential antigenic target for vaccinal purposes against sporotrichosis. |
format | Online Article Text |
id | pubmed-6868355 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-68683552019-12-04 Immunization with recombinant enolase of Sporothrix spp. (rSsEno) confers effective protection against sporotrichosis in mice Portuondo, Deivys Leandro Dores-Silva, Paulo Roberto Ferreira, Lucas Souza de Oliveira, Carlos S. Téllez-Martínez, Damiana Marcos, Caroline Maria de Aguiar Loesch, Maria Luiza Guzmán, Fanny Gava, Lisandra M. Borges, Júlio César Pereira, Sandro Antonio Batista-Duharte, Alexander Carlos, Iracilda Zeppone Sci Rep Article In recent years, research has focused on the immunoreactive components of the Sporothrix schenckii cell wall that can be relevant targets for preventive and therapeutic vaccines against sporotrichosis, an emergent worldwide mycosis. In a previous study, we identified a 47-kDa enolase as an immunodominant antigen in mice vaccinated with an adjuvanted mixture of S. schenckii cell wall proteins. Here, we sought to assess the protective potential of a Sporothrix spp. recombinant enolase (rSsEno) formulated with or without the adjuvant Montanide Pet-GelA (PGA) against the S. brasiliensis infection in mice. Mice that were immunized with rSsEno plus PGA showed increased antibody titters against rSsEno and increased median survival time when challenged with S. brasiliensis as compared with mice that had not been immunized or that were immunized with rSsEno alone. Immunization with rSsEno plus PGA induced a predominantly T-helper 1 cytokine pattern after in vitro stimulation of splenic cells with rSsEno: elevated levels of IFN-γ and IL-2, as well as of other cytokines involved in host defense against sporotrichosis, such as TNF-alpha, IL-6, and IL-4. Furthermore, we show for the first time the presence of enolase in the cell wall of both S. schenckii and S. brasiliensis. As a whole, our results suggest that enolase could be used as a potential antigenic target for vaccinal purposes against sporotrichosis. Nature Publishing Group UK 2019-11-20 /pmc/articles/PMC6868355/ /pubmed/31748544 http://dx.doi.org/10.1038/s41598-019-53135-z Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Portuondo, Deivys Leandro Dores-Silva, Paulo Roberto Ferreira, Lucas Souza de Oliveira, Carlos S. Téllez-Martínez, Damiana Marcos, Caroline Maria de Aguiar Loesch, Maria Luiza Guzmán, Fanny Gava, Lisandra M. Borges, Júlio César Pereira, Sandro Antonio Batista-Duharte, Alexander Carlos, Iracilda Zeppone Immunization with recombinant enolase of Sporothrix spp. (rSsEno) confers effective protection against sporotrichosis in mice |
title | Immunization with recombinant enolase of Sporothrix spp. (rSsEno) confers effective protection against sporotrichosis in mice |
title_full | Immunization with recombinant enolase of Sporothrix spp. (rSsEno) confers effective protection against sporotrichosis in mice |
title_fullStr | Immunization with recombinant enolase of Sporothrix spp. (rSsEno) confers effective protection against sporotrichosis in mice |
title_full_unstemmed | Immunization with recombinant enolase of Sporothrix spp. (rSsEno) confers effective protection against sporotrichosis in mice |
title_short | Immunization with recombinant enolase of Sporothrix spp. (rSsEno) confers effective protection against sporotrichosis in mice |
title_sort | immunization with recombinant enolase of sporothrix spp. (rsseno) confers effective protection against sporotrichosis in mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6868355/ https://www.ncbi.nlm.nih.gov/pubmed/31748544 http://dx.doi.org/10.1038/s41598-019-53135-z |
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