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A surface plasmon resonance immunoassay for the rapid analysis of methamphetamine in forensic oral fluid
BACKGROUND: Current chromatographic methods applied for the forensic analysis of methamphetamine are costly, time‐consuming, and require complicated pretreatment procedures. Thus, the rapid detection of methamphetamine is a critical and unmet need. In this study, a surface plasmon resonance (SPR) sy...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6868402/ https://www.ncbi.nlm.nih.gov/pubmed/31373733 http://dx.doi.org/10.1002/jcla.22993 |
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author | Wang, Jiye Yao, Weixuan Meng, Fanwei Wang, Pengjuan Wu, Yuanzhao Wang, Binjie |
author_facet | Wang, Jiye Yao, Weixuan Meng, Fanwei Wang, Pengjuan Wu, Yuanzhao Wang, Binjie |
author_sort | Wang, Jiye |
collection | PubMed |
description | BACKGROUND: Current chromatographic methods applied for the forensic analysis of methamphetamine are costly, time‐consuming, and require complicated pretreatment procedures. Thus, the rapid detection of methamphetamine is a critical and unmet need. In this study, a surface plasmon resonance (SPR) system based on indirect inhibitive immunoassay was designed for the analysis of methamphetamine in forensic oral fluid samples. METHODS: For the inhibition immunoassay, the diluted oral fluid was mixed with methamphetamine antibody and then injected into the SPR sensor chip. The biosensor chip was constructed by covalently immobilizing of methamphetamine‐bovine serum albumin conjugate onto a carboxymethyl dextran surface at an optimized pH. The concentration of antibody was also optimized. RESULTS: The SPR biosensor showed good sensitivity with a limit of detection of 0.44 ng/mL and was comparable or lower than the pre‐existing methods. The method was finally tested using oral fluid samples from 20 suspected drug abusers in forensic cases, and it provided an acceptable recovery of 113.2%, indicating good anti‐interference capability of the SPR sensor. CONCLUSION: The SPR biosensor was rapid, reproducible, and had a great potential approach for the forensic detection of methamphetamine. |
format | Online Article Text |
id | pubmed-6868402 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-68684022019-11-25 A surface plasmon resonance immunoassay for the rapid analysis of methamphetamine in forensic oral fluid Wang, Jiye Yao, Weixuan Meng, Fanwei Wang, Pengjuan Wu, Yuanzhao Wang, Binjie J Clin Lab Anal Research Articles BACKGROUND: Current chromatographic methods applied for the forensic analysis of methamphetamine are costly, time‐consuming, and require complicated pretreatment procedures. Thus, the rapid detection of methamphetamine is a critical and unmet need. In this study, a surface plasmon resonance (SPR) system based on indirect inhibitive immunoassay was designed for the analysis of methamphetamine in forensic oral fluid samples. METHODS: For the inhibition immunoassay, the diluted oral fluid was mixed with methamphetamine antibody and then injected into the SPR sensor chip. The biosensor chip was constructed by covalently immobilizing of methamphetamine‐bovine serum albumin conjugate onto a carboxymethyl dextran surface at an optimized pH. The concentration of antibody was also optimized. RESULTS: The SPR biosensor showed good sensitivity with a limit of detection of 0.44 ng/mL and was comparable or lower than the pre‐existing methods. The method was finally tested using oral fluid samples from 20 suspected drug abusers in forensic cases, and it provided an acceptable recovery of 113.2%, indicating good anti‐interference capability of the SPR sensor. CONCLUSION: The SPR biosensor was rapid, reproducible, and had a great potential approach for the forensic detection of methamphetamine. John Wiley and Sons Inc. 2019-08-02 /pmc/articles/PMC6868402/ /pubmed/31373733 http://dx.doi.org/10.1002/jcla.22993 Text en © 2019 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals, Inc. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Wang, Jiye Yao, Weixuan Meng, Fanwei Wang, Pengjuan Wu, Yuanzhao Wang, Binjie A surface plasmon resonance immunoassay for the rapid analysis of methamphetamine in forensic oral fluid |
title | A surface plasmon resonance immunoassay for the rapid analysis of methamphetamine in forensic oral fluid |
title_full | A surface plasmon resonance immunoassay for the rapid analysis of methamphetamine in forensic oral fluid |
title_fullStr | A surface plasmon resonance immunoassay for the rapid analysis of methamphetamine in forensic oral fluid |
title_full_unstemmed | A surface plasmon resonance immunoassay for the rapid analysis of methamphetamine in forensic oral fluid |
title_short | A surface plasmon resonance immunoassay for the rapid analysis of methamphetamine in forensic oral fluid |
title_sort | surface plasmon resonance immunoassay for the rapid analysis of methamphetamine in forensic oral fluid |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6868402/ https://www.ncbi.nlm.nih.gov/pubmed/31373733 http://dx.doi.org/10.1002/jcla.22993 |
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