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Detection of non‐criteria autoantibodies in women without apparent causes for pregnancy loss

BACKGROUND: Some of the non‐criteria autoantibodies, especially non‐conventional antiphospholipid (aPL) antibodies, were present with high prevalence in sporadic miscarriages and recurrent pregnant loss. However, whether these autoantibodies are associated with miscarriage patients without apparent...

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Autores principales: Zhu, Hongyuan, Wang, Meng, Dong, Ying, Hu, Honghai, Zhang, Qiaoli, Qiao, Chen, Xie, Xin, Fan, Fei, Zeng, Jiazi, Jia, Yan, Chen, Lu, Liu, Jingrui, Li, Lin, Zhai, Yanhong, Zhao, Zhen, Shen, Min, Cao, Zheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6868457/
https://www.ncbi.nlm.nih.gov/pubmed/31402484
http://dx.doi.org/10.1002/jcla.22994
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author Zhu, Hongyuan
Wang, Meng
Dong, Ying
Hu, Honghai
Zhang, Qiaoli
Qiao, Chen
Xie, Xin
Fan, Fei
Zeng, Jiazi
Jia, Yan
Chen, Lu
Liu, Jingrui
Li, Lin
Zhai, Yanhong
Zhao, Zhen
Shen, Min
Cao, Zheng
author_facet Zhu, Hongyuan
Wang, Meng
Dong, Ying
Hu, Honghai
Zhang, Qiaoli
Qiao, Chen
Xie, Xin
Fan, Fei
Zeng, Jiazi
Jia, Yan
Chen, Lu
Liu, Jingrui
Li, Lin
Zhai, Yanhong
Zhao, Zhen
Shen, Min
Cao, Zheng
author_sort Zhu, Hongyuan
collection PubMed
description BACKGROUND: Some of the non‐criteria autoantibodies, especially non‐conventional antiphospholipid (aPL) antibodies, were present with high prevalence in sporadic miscarriages and recurrent pregnant loss. However, whether these autoantibodies are associated with miscarriage patients without apparent causes remain unclear. METHODS: The subjects were recruited from the female patients visiting the Infertility Center at the Beijing Obstetrics and Gynecology Hospital from January 2017 to March 2018. The women who experienced one sporadic miscarriage (n = 89) or recurrent pregnancy loss (n = 125) were enrolled. The control participants (n = 59) were those women with normal pregnancy history and with no miscarriage or thrombosis experience. The collected serum specimens from above patients and controls were subjected to the 13 non‐criteria autoantibody examinations, targeting non‐conventional phospholipids, thyroid, sperm, endometrial, and anti‐nuclear antigens. RESULTS: When compared with the controls, the following non‐criteria antibodies stood out in present study with significantly increased frequency and were listed in the order of decreasing positive rates: aPE IgM (40.0%), ANA (15.2%), aEM IgG (13.6%), aPE IgG (12.8%), and aPT IgM (10.4%). Except for ANA, the presence of aPE IgM, aEM IgG, aPE IgG, and aPT IgM was not associated with positivity of LA tests. In receiver operating characteristic analyses, the combined aPE IgG and aEM IgG biomarker panel had the best discriminating power between miscarriage patients and healthy controls. CONCLUSION: Our findings suggested that the non‐criteria could be included as part of the pregnancy loss evaluation when apparent causes are absent, and the conventional aPLs tests failed to provide interpretations.
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spelling pubmed-68684572019-11-25 Detection of non‐criteria autoantibodies in women without apparent causes for pregnancy loss Zhu, Hongyuan Wang, Meng Dong, Ying Hu, Honghai Zhang, Qiaoli Qiao, Chen Xie, Xin Fan, Fei Zeng, Jiazi Jia, Yan Chen, Lu Liu, Jingrui Li, Lin Zhai, Yanhong Zhao, Zhen Shen, Min Cao, Zheng J Clin Lab Anal Research Articles BACKGROUND: Some of the non‐criteria autoantibodies, especially non‐conventional antiphospholipid (aPL) antibodies, were present with high prevalence in sporadic miscarriages and recurrent pregnant loss. However, whether these autoantibodies are associated with miscarriage patients without apparent causes remain unclear. METHODS: The subjects were recruited from the female patients visiting the Infertility Center at the Beijing Obstetrics and Gynecology Hospital from January 2017 to March 2018. The women who experienced one sporadic miscarriage (n = 89) or recurrent pregnancy loss (n = 125) were enrolled. The control participants (n = 59) were those women with normal pregnancy history and with no miscarriage or thrombosis experience. The collected serum specimens from above patients and controls were subjected to the 13 non‐criteria autoantibody examinations, targeting non‐conventional phospholipids, thyroid, sperm, endometrial, and anti‐nuclear antigens. RESULTS: When compared with the controls, the following non‐criteria antibodies stood out in present study with significantly increased frequency and were listed in the order of decreasing positive rates: aPE IgM (40.0%), ANA (15.2%), aEM IgG (13.6%), aPE IgG (12.8%), and aPT IgM (10.4%). Except for ANA, the presence of aPE IgM, aEM IgG, aPE IgG, and aPT IgM was not associated with positivity of LA tests. In receiver operating characteristic analyses, the combined aPE IgG and aEM IgG biomarker panel had the best discriminating power between miscarriage patients and healthy controls. CONCLUSION: Our findings suggested that the non‐criteria could be included as part of the pregnancy loss evaluation when apparent causes are absent, and the conventional aPLs tests failed to provide interpretations. John Wiley and Sons Inc. 2019-08-11 /pmc/articles/PMC6868457/ /pubmed/31402484 http://dx.doi.org/10.1002/jcla.22994 Text en © 2019 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals, Inc. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Zhu, Hongyuan
Wang, Meng
Dong, Ying
Hu, Honghai
Zhang, Qiaoli
Qiao, Chen
Xie, Xin
Fan, Fei
Zeng, Jiazi
Jia, Yan
Chen, Lu
Liu, Jingrui
Li, Lin
Zhai, Yanhong
Zhao, Zhen
Shen, Min
Cao, Zheng
Detection of non‐criteria autoantibodies in women without apparent causes for pregnancy loss
title Detection of non‐criteria autoantibodies in women without apparent causes for pregnancy loss
title_full Detection of non‐criteria autoantibodies in women without apparent causes for pregnancy loss
title_fullStr Detection of non‐criteria autoantibodies in women without apparent causes for pregnancy loss
title_full_unstemmed Detection of non‐criteria autoantibodies in women without apparent causes for pregnancy loss
title_short Detection of non‐criteria autoantibodies in women without apparent causes for pregnancy loss
title_sort detection of non‐criteria autoantibodies in women without apparent causes for pregnancy loss
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6868457/
https://www.ncbi.nlm.nih.gov/pubmed/31402484
http://dx.doi.org/10.1002/jcla.22994
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