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Proteomic Analysis of the Xanthan-Degrading Pathway of Microbacterium sp. XT11
[Image: see text] Xanthan, a highly stable polysaccharide which is not easily degraded by most microorganisms, contains a cellulosic backbone with trisaccharide side chains composed of mannosyl-glucuronyl-mannose attached α-1,3 to alternating glucosyl residues. Different digestion strategies were fi...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2019
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6868878/ https://www.ncbi.nlm.nih.gov/pubmed/31763532 http://dx.doi.org/10.1021/acsomega.9b02313 |
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author | Sun, Zhen Liu, Huixue Wang, Xueyan Yang, Fan Li, Xianzhen |
author_facet | Sun, Zhen Liu, Huixue Wang, Xueyan Yang, Fan Li, Xianzhen |
author_sort | Sun, Zhen |
collection | PubMed |
description | [Image: see text] Xanthan, a highly stable polysaccharide which is not easily degraded by most microorganisms, contains a cellulosic backbone with trisaccharide side chains composed of mannosyl-glucuronyl-mannose attached α-1,3 to alternating glucosyl residues. Different digestion strategies were first applied to demonstrate the complexity about the proteomes of Microbacterium sp. XT11 in xanthan medium and glucose medium. Significantly up-regulated proteins induced by xanthan were screened out by the label-free quantitation of the proteomes of Microbacterium sp. XT11 in xanthan medium and glucose medium. Consequently, 2746 and 2878 proteins were identified in proteomes of Microbacterium sp. XT11 in xanthan medium and glucose medium individually, which represent 80.6 and 84.4% of total protein dataset predicted to be expressed by the gene. In the list of 430 induced proteins containing the proteins specifically expressed or up-regulated in xanthan medium, 19 proteins involved in carbohydrate-active enzymes database and 38 proteins annotated with transporter activity were critical in the degrading pathway of xanthan. Four CAZymes (GH3, GH38, GH9, and PL8) and one ABC transporter (LX1-1GL001097) were verified with quantitative real-time polymerase chain reaction. Four CAZymes (GH3, GH38, GH9, and PL8) were further verified with the enzyme assay. This study suggests a xanthan-degrading pathway in Microbacterium sp. XT11, and other potential xanthan degradation-related proteins still need further investigation. |
format | Online Article Text |
id | pubmed-6868878 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-68688782019-11-22 Proteomic Analysis of the Xanthan-Degrading Pathway of Microbacterium sp. XT11 Sun, Zhen Liu, Huixue Wang, Xueyan Yang, Fan Li, Xianzhen ACS Omega [Image: see text] Xanthan, a highly stable polysaccharide which is not easily degraded by most microorganisms, contains a cellulosic backbone with trisaccharide side chains composed of mannosyl-glucuronyl-mannose attached α-1,3 to alternating glucosyl residues. Different digestion strategies were first applied to demonstrate the complexity about the proteomes of Microbacterium sp. XT11 in xanthan medium and glucose medium. Significantly up-regulated proteins induced by xanthan were screened out by the label-free quantitation of the proteomes of Microbacterium sp. XT11 in xanthan medium and glucose medium. Consequently, 2746 and 2878 proteins were identified in proteomes of Microbacterium sp. XT11 in xanthan medium and glucose medium individually, which represent 80.6 and 84.4% of total protein dataset predicted to be expressed by the gene. In the list of 430 induced proteins containing the proteins specifically expressed or up-regulated in xanthan medium, 19 proteins involved in carbohydrate-active enzymes database and 38 proteins annotated with transporter activity were critical in the degrading pathway of xanthan. Four CAZymes (GH3, GH38, GH9, and PL8) and one ABC transporter (LX1-1GL001097) were verified with quantitative real-time polymerase chain reaction. Four CAZymes (GH3, GH38, GH9, and PL8) were further verified with the enzyme assay. This study suggests a xanthan-degrading pathway in Microbacterium sp. XT11, and other potential xanthan degradation-related proteins still need further investigation. American Chemical Society 2019-11-06 /pmc/articles/PMC6868878/ /pubmed/31763532 http://dx.doi.org/10.1021/acsomega.9b02313 Text en Copyright © 2019 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes. |
spellingShingle | Sun, Zhen Liu, Huixue Wang, Xueyan Yang, Fan Li, Xianzhen Proteomic Analysis of the Xanthan-Degrading Pathway of Microbacterium sp. XT11 |
title | Proteomic Analysis of the Xanthan-Degrading Pathway
of Microbacterium sp. XT11 |
title_full | Proteomic Analysis of the Xanthan-Degrading Pathway
of Microbacterium sp. XT11 |
title_fullStr | Proteomic Analysis of the Xanthan-Degrading Pathway
of Microbacterium sp. XT11 |
title_full_unstemmed | Proteomic Analysis of the Xanthan-Degrading Pathway
of Microbacterium sp. XT11 |
title_short | Proteomic Analysis of the Xanthan-Degrading Pathway
of Microbacterium sp. XT11 |
title_sort | proteomic analysis of the xanthan-degrading pathway
of microbacterium sp. xt11 |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6868878/ https://www.ncbi.nlm.nih.gov/pubmed/31763532 http://dx.doi.org/10.1021/acsomega.9b02313 |
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