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Monitoring of diffusion properties and transverse relaxation time of mouse ischaemic muscle after administration of human mesenchymal stromal cells derived from adipose tissue

OBJECTIVES: Application of non‐invasive imaging methods plays an important role in the assessment of cellular therapy effects in peripheral artery disease. The purpose of this work was to evaluate the kinetics of MRI‐derived parameters characterizing ischaemic hindlimb muscle after administration of...

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Autores principales: Skorupa, Agnieszka, Ciszek, Mateusz, Pilny, Ewelina, Smolarczyk, Ryszard, Jarosz–Biej, Magdalena, Boguszewicz, Łukasz, Krakowczyk, Łukasz, Szala, Stanisław, Sokół, Maria, Cichoń, Tomasz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6869084/
https://www.ncbi.nlm.nih.gov/pubmed/31441162
http://dx.doi.org/10.1111/cpr.12672
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author Skorupa, Agnieszka
Ciszek, Mateusz
Pilny, Ewelina
Smolarczyk, Ryszard
Jarosz–Biej, Magdalena
Boguszewicz, Łukasz
Krakowczyk, Łukasz
Szala, Stanisław
Sokół, Maria
Cichoń, Tomasz
author_facet Skorupa, Agnieszka
Ciszek, Mateusz
Pilny, Ewelina
Smolarczyk, Ryszard
Jarosz–Biej, Magdalena
Boguszewicz, Łukasz
Krakowczyk, Łukasz
Szala, Stanisław
Sokół, Maria
Cichoń, Tomasz
author_sort Skorupa, Agnieszka
collection PubMed
description OBJECTIVES: Application of non‐invasive imaging methods plays an important role in the assessment of cellular therapy effects in peripheral artery disease. The purpose of this work was to evaluate the kinetics of MRI‐derived parameters characterizing ischaemic hindlimb muscle after administration of human mesenchymal stromal cells derived from adipose tissue (hADSC) in mice. MATERIALS AND METHODS: MRI experiments were performed on a 9.4T Bruker system. The measurement protocol included transverse relaxation time mapping and diffusion tensor imaging. The monitoring period encompassed 14 days after femoral artery ligation and subsequent cell administration. The effect of hADSC transplantation was compared with the effect of normal human dermal fibroblasts (NHDFs) and phosphate‐buffered saline injection. RESULTS: The most significant differences between the hADSC group and the remaining ones were observed around day 3 after ischaemia induction (increased transverse relaxation time in the hADSC group in comparison with the control group) and around day 7 (increased transverse relaxation time and decreased third eigenvalue of the diffusion tensor in the hADSC group in comparison with the control and NHDF groups) at the site of hADSC injection. Histologically, it was associated with increased macrophage infiltration at days 3‐7 and with the presence of small regenerating fibres in the ischaemic tissue at day 7. CONCLUSIONS: Our results underscore the important role of macrophages in mediating the therapeutic effects of hADSCs and confirm the huge potential of magnetic resonance imaging in monitoring of cellular therapy effects.
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spelling pubmed-68690842020-03-13 Monitoring of diffusion properties and transverse relaxation time of mouse ischaemic muscle after administration of human mesenchymal stromal cells derived from adipose tissue Skorupa, Agnieszka Ciszek, Mateusz Pilny, Ewelina Smolarczyk, Ryszard Jarosz–Biej, Magdalena Boguszewicz, Łukasz Krakowczyk, Łukasz Szala, Stanisław Sokół, Maria Cichoń, Tomasz Cell Prolif Original Articles OBJECTIVES: Application of non‐invasive imaging methods plays an important role in the assessment of cellular therapy effects in peripheral artery disease. The purpose of this work was to evaluate the kinetics of MRI‐derived parameters characterizing ischaemic hindlimb muscle after administration of human mesenchymal stromal cells derived from adipose tissue (hADSC) in mice. MATERIALS AND METHODS: MRI experiments were performed on a 9.4T Bruker system. The measurement protocol included transverse relaxation time mapping and diffusion tensor imaging. The monitoring period encompassed 14 days after femoral artery ligation and subsequent cell administration. The effect of hADSC transplantation was compared with the effect of normal human dermal fibroblasts (NHDFs) and phosphate‐buffered saline injection. RESULTS: The most significant differences between the hADSC group and the remaining ones were observed around day 3 after ischaemia induction (increased transverse relaxation time in the hADSC group in comparison with the control group) and around day 7 (increased transverse relaxation time and decreased third eigenvalue of the diffusion tensor in the hADSC group in comparison with the control and NHDF groups) at the site of hADSC injection. Histologically, it was associated with increased macrophage infiltration at days 3‐7 and with the presence of small regenerating fibres in the ischaemic tissue at day 7. CONCLUSIONS: Our results underscore the important role of macrophages in mediating the therapeutic effects of hADSCs and confirm the huge potential of magnetic resonance imaging in monitoring of cellular therapy effects. John Wiley and Sons Inc. 2019-08-23 /pmc/articles/PMC6869084/ /pubmed/31441162 http://dx.doi.org/10.1111/cpr.12672 Text en © 2019 The Authors. Cell Proliferation Published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Skorupa, Agnieszka
Ciszek, Mateusz
Pilny, Ewelina
Smolarczyk, Ryszard
Jarosz–Biej, Magdalena
Boguszewicz, Łukasz
Krakowczyk, Łukasz
Szala, Stanisław
Sokół, Maria
Cichoń, Tomasz
Monitoring of diffusion properties and transverse relaxation time of mouse ischaemic muscle after administration of human mesenchymal stromal cells derived from adipose tissue
title Monitoring of diffusion properties and transverse relaxation time of mouse ischaemic muscle after administration of human mesenchymal stromal cells derived from adipose tissue
title_full Monitoring of diffusion properties and transverse relaxation time of mouse ischaemic muscle after administration of human mesenchymal stromal cells derived from adipose tissue
title_fullStr Monitoring of diffusion properties and transverse relaxation time of mouse ischaemic muscle after administration of human mesenchymal stromal cells derived from adipose tissue
title_full_unstemmed Monitoring of diffusion properties and transverse relaxation time of mouse ischaemic muscle after administration of human mesenchymal stromal cells derived from adipose tissue
title_short Monitoring of diffusion properties and transverse relaxation time of mouse ischaemic muscle after administration of human mesenchymal stromal cells derived from adipose tissue
title_sort monitoring of diffusion properties and transverse relaxation time of mouse ischaemic muscle after administration of human mesenchymal stromal cells derived from adipose tissue
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6869084/
https://www.ncbi.nlm.nih.gov/pubmed/31441162
http://dx.doi.org/10.1111/cpr.12672
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