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Comparative iTRAQ proteomics revealed proteins associated with lobed fin regeneration in Bichirs

BACKGROUND: Polypterus senegalus can fully regenerate its pectoral lobed fins, including a complex endoskeleton, with remarkable precision. However, despite the enormous potential of this species for use in medical research, its regeneration mechanisms remain largely unknown. METHODS: To identify th...

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Autores principales: Lu, Suxiang, Xiong, Qian, Du, Kang, Gan, Xiaoni, Wang, Xuzhen, Yang, Liandong, Wang, Ying, Ge, Feng, He, Shunping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6869209/
https://www.ncbi.nlm.nih.gov/pubmed/31832023
http://dx.doi.org/10.1186/s12953-019-0153-0
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author Lu, Suxiang
Xiong, Qian
Du, Kang
Gan, Xiaoni
Wang, Xuzhen
Yang, Liandong
Wang, Ying
Ge, Feng
He, Shunping
author_facet Lu, Suxiang
Xiong, Qian
Du, Kang
Gan, Xiaoni
Wang, Xuzhen
Yang, Liandong
Wang, Ying
Ge, Feng
He, Shunping
author_sort Lu, Suxiang
collection PubMed
description BACKGROUND: Polypterus senegalus can fully regenerate its pectoral lobed fins, including a complex endoskeleton, with remarkable precision. However, despite the enormous potential of this species for use in medical research, its regeneration mechanisms remain largely unknown. METHODS: To identify the differentially expressed proteins (DEPs) during the early stages of lobed fin regeneration in P. senegalus, we performed a differential proteomic analysis using isobaric tag for relative and absolute quantitation (iTRAQ) approach based quantitative proteome from the pectoral lobed fins at 3 time points. Furthermore, we validated the changes in protein expression with multiple-reaction monitoring (MRM) analysis. RESULTS: The experiment yielded a total of 3177 proteins and 15,091 unique peptides including 1006 non-redundant (nr) DEPs. Of these, 592 were upregulated while 349 were downregulated after lobed fin amputation when compared to the original tissue. Bioinformatics analyses showed that the DEPs were mainly associated with Ribosome and RNA transport, metabolic, ECM-receptor interaction, Golgi and endoplasmic reticulum, DNA replication, and Regulation of actin cytoskeleton. CONCLUSIONS: To our knowledge, this is the first proteomic research to investigate alterations in protein levels and affected pathways in bichirs’ lobe-fin/limb regeneration. In addition, our study demonstrated a highly dynamic regulation during lobed fin regeneration in P. senegalus. These results not only provide a comprehensive dataset on differentially expressed proteins during the early stages of lobe-fin/limb regeneration but also advance our understanding of the molecular mechanisms underlying lobe-fin/limb regeneration.
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spelling pubmed-68692092019-12-12 Comparative iTRAQ proteomics revealed proteins associated with lobed fin regeneration in Bichirs Lu, Suxiang Xiong, Qian Du, Kang Gan, Xiaoni Wang, Xuzhen Yang, Liandong Wang, Ying Ge, Feng He, Shunping Proteome Sci Research BACKGROUND: Polypterus senegalus can fully regenerate its pectoral lobed fins, including a complex endoskeleton, with remarkable precision. However, despite the enormous potential of this species for use in medical research, its regeneration mechanisms remain largely unknown. METHODS: To identify the differentially expressed proteins (DEPs) during the early stages of lobed fin regeneration in P. senegalus, we performed a differential proteomic analysis using isobaric tag for relative and absolute quantitation (iTRAQ) approach based quantitative proteome from the pectoral lobed fins at 3 time points. Furthermore, we validated the changes in protein expression with multiple-reaction monitoring (MRM) analysis. RESULTS: The experiment yielded a total of 3177 proteins and 15,091 unique peptides including 1006 non-redundant (nr) DEPs. Of these, 592 were upregulated while 349 were downregulated after lobed fin amputation when compared to the original tissue. Bioinformatics analyses showed that the DEPs were mainly associated with Ribosome and RNA transport, metabolic, ECM-receptor interaction, Golgi and endoplasmic reticulum, DNA replication, and Regulation of actin cytoskeleton. CONCLUSIONS: To our knowledge, this is the first proteomic research to investigate alterations in protein levels and affected pathways in bichirs’ lobe-fin/limb regeneration. In addition, our study demonstrated a highly dynamic regulation during lobed fin regeneration in P. senegalus. These results not only provide a comprehensive dataset on differentially expressed proteins during the early stages of lobe-fin/limb regeneration but also advance our understanding of the molecular mechanisms underlying lobe-fin/limb regeneration. BioMed Central 2019-11-20 /pmc/articles/PMC6869209/ /pubmed/31832023 http://dx.doi.org/10.1186/s12953-019-0153-0 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Lu, Suxiang
Xiong, Qian
Du, Kang
Gan, Xiaoni
Wang, Xuzhen
Yang, Liandong
Wang, Ying
Ge, Feng
He, Shunping
Comparative iTRAQ proteomics revealed proteins associated with lobed fin regeneration in Bichirs
title Comparative iTRAQ proteomics revealed proteins associated with lobed fin regeneration in Bichirs
title_full Comparative iTRAQ proteomics revealed proteins associated with lobed fin regeneration in Bichirs
title_fullStr Comparative iTRAQ proteomics revealed proteins associated with lobed fin regeneration in Bichirs
title_full_unstemmed Comparative iTRAQ proteomics revealed proteins associated with lobed fin regeneration in Bichirs
title_short Comparative iTRAQ proteomics revealed proteins associated with lobed fin regeneration in Bichirs
title_sort comparative itraq proteomics revealed proteins associated with lobed fin regeneration in bichirs
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6869209/
https://www.ncbi.nlm.nih.gov/pubmed/31832023
http://dx.doi.org/10.1186/s12953-019-0153-0
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