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The genomes of two parasitic wasps that parasitize the diamondback moth

BACKGROUND: Parasitic insects are well-known biological control agents for arthropod pests worldwide. They are capable of regulating their host’s physiology, development and behaviour. However, many of the molecular mechanisms involved in host-parasitoid interaction remain unknown. RESULTS: We seque...

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Autores principales: Shi, Min, Wang, Zhizhi, Ye, Xiqian, Xie, Hongqing, Li, Fei, Hu, Xiaoxiao, Wang, Zehua, Yin, Chuanlin, Zhou, Yuenan, Gu, Qijuan, Zou, Jiani, Zhan, Leqing, Yao, Yuan, Yang, Jian, Wei, Shujun, Hu, Rongmin, Guo, Dianhao, Zhu, Jiangyan, Wang, Yanping, Huang, Jianhua, Pennacchio, Francesco, Strand, Michael R., Chen, Xuexin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6873472/
https://www.ncbi.nlm.nih.gov/pubmed/31752718
http://dx.doi.org/10.1186/s12864-019-6266-0
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author Shi, Min
Wang, Zhizhi
Ye, Xiqian
Xie, Hongqing
Li, Fei
Hu, Xiaoxiao
Wang, Zehua
Yin, Chuanlin
Zhou, Yuenan
Gu, Qijuan
Zou, Jiani
Zhan, Leqing
Yao, Yuan
Yang, Jian
Wei, Shujun
Hu, Rongmin
Guo, Dianhao
Zhu, Jiangyan
Wang, Yanping
Huang, Jianhua
Pennacchio, Francesco
Strand, Michael R.
Chen, Xuexin
author_facet Shi, Min
Wang, Zhizhi
Ye, Xiqian
Xie, Hongqing
Li, Fei
Hu, Xiaoxiao
Wang, Zehua
Yin, Chuanlin
Zhou, Yuenan
Gu, Qijuan
Zou, Jiani
Zhan, Leqing
Yao, Yuan
Yang, Jian
Wei, Shujun
Hu, Rongmin
Guo, Dianhao
Zhu, Jiangyan
Wang, Yanping
Huang, Jianhua
Pennacchio, Francesco
Strand, Michael R.
Chen, Xuexin
author_sort Shi, Min
collection PubMed
description BACKGROUND: Parasitic insects are well-known biological control agents for arthropod pests worldwide. They are capable of regulating their host’s physiology, development and behaviour. However, many of the molecular mechanisms involved in host-parasitoid interaction remain unknown. RESULTS: We sequenced the genomes of two parasitic wasps (Cotesia vestalis, and Diadromus collaris) that parasitize the diamondback moth Plutella xylostella using Illumina and Pacbio sequencing platforms. Genome assembly using SOAPdenovo produced a 178 Mb draft genome for C. vestalis and a 399 Mb draft genome for D. collaris. A total set that contained 11,278 and 15,328 protein-coding genes for C. vestalis and D. collaris, respectively, were predicted using evidence (homology-based and transcriptome-based) and de novo prediction methodology. Phylogenetic analysis showed that the braconid C. vestalis and the ichneumonid D. collaris diverged approximately 124 million years ago. These two wasps exhibit gene gains and losses that in some cases reflect their shared life history as parasitic wasps and in other cases are unique to particular species. Gene families with functions in development, nutrient acquisition from hosts, and metabolism have expanded in each wasp species, while genes required for biosynthesis of some amino acids and steroids have been lost, since these nutrients can be directly obtained from the host. Both wasp species encode a relative higher number of neprilysins (NEPs) thus far reported in arthropod genomes while several genes encoding immune-related proteins and detoxification enzymes were lost in both wasp genomes. CONCLUSIONS: We present the annotated genome sequence of two parasitic wasps C. vestalis and D. collaris, which parasitize a common host, the diamondback moth, P. xylostella. These data will provide a fundamental source for studying the mechanism of host control and will be used in parasitoid comparative genomics to study the origin and diversification of the parasitic lifestyle.
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spelling pubmed-68734722019-12-12 The genomes of two parasitic wasps that parasitize the diamondback moth Shi, Min Wang, Zhizhi Ye, Xiqian Xie, Hongqing Li, Fei Hu, Xiaoxiao Wang, Zehua Yin, Chuanlin Zhou, Yuenan Gu, Qijuan Zou, Jiani Zhan, Leqing Yao, Yuan Yang, Jian Wei, Shujun Hu, Rongmin Guo, Dianhao Zhu, Jiangyan Wang, Yanping Huang, Jianhua Pennacchio, Francesco Strand, Michael R. Chen, Xuexin BMC Genomics Research Article BACKGROUND: Parasitic insects are well-known biological control agents for arthropod pests worldwide. They are capable of regulating their host’s physiology, development and behaviour. However, many of the molecular mechanisms involved in host-parasitoid interaction remain unknown. RESULTS: We sequenced the genomes of two parasitic wasps (Cotesia vestalis, and Diadromus collaris) that parasitize the diamondback moth Plutella xylostella using Illumina and Pacbio sequencing platforms. Genome assembly using SOAPdenovo produced a 178 Mb draft genome for C. vestalis and a 399 Mb draft genome for D. collaris. A total set that contained 11,278 and 15,328 protein-coding genes for C. vestalis and D. collaris, respectively, were predicted using evidence (homology-based and transcriptome-based) and de novo prediction methodology. Phylogenetic analysis showed that the braconid C. vestalis and the ichneumonid D. collaris diverged approximately 124 million years ago. These two wasps exhibit gene gains and losses that in some cases reflect their shared life history as parasitic wasps and in other cases are unique to particular species. Gene families with functions in development, nutrient acquisition from hosts, and metabolism have expanded in each wasp species, while genes required for biosynthesis of some amino acids and steroids have been lost, since these nutrients can be directly obtained from the host. Both wasp species encode a relative higher number of neprilysins (NEPs) thus far reported in arthropod genomes while several genes encoding immune-related proteins and detoxification enzymes were lost in both wasp genomes. CONCLUSIONS: We present the annotated genome sequence of two parasitic wasps C. vestalis and D. collaris, which parasitize a common host, the diamondback moth, P. xylostella. These data will provide a fundamental source for studying the mechanism of host control and will be used in parasitoid comparative genomics to study the origin and diversification of the parasitic lifestyle. BioMed Central 2019-11-21 /pmc/articles/PMC6873472/ /pubmed/31752718 http://dx.doi.org/10.1186/s12864-019-6266-0 Text en © The Author(s). 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Shi, Min
Wang, Zhizhi
Ye, Xiqian
Xie, Hongqing
Li, Fei
Hu, Xiaoxiao
Wang, Zehua
Yin, Chuanlin
Zhou, Yuenan
Gu, Qijuan
Zou, Jiani
Zhan, Leqing
Yao, Yuan
Yang, Jian
Wei, Shujun
Hu, Rongmin
Guo, Dianhao
Zhu, Jiangyan
Wang, Yanping
Huang, Jianhua
Pennacchio, Francesco
Strand, Michael R.
Chen, Xuexin
The genomes of two parasitic wasps that parasitize the diamondback moth
title The genomes of two parasitic wasps that parasitize the diamondback moth
title_full The genomes of two parasitic wasps that parasitize the diamondback moth
title_fullStr The genomes of two parasitic wasps that parasitize the diamondback moth
title_full_unstemmed The genomes of two parasitic wasps that parasitize the diamondback moth
title_short The genomes of two parasitic wasps that parasitize the diamondback moth
title_sort genomes of two parasitic wasps that parasitize the diamondback moth
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6873472/
https://www.ncbi.nlm.nih.gov/pubmed/31752718
http://dx.doi.org/10.1186/s12864-019-6266-0
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