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Effect of photoinitiator on chain degradation of hyaluronic acid
OBJECTIVES: Photocrosslinking systems of polymers have been widely studied using UV or visible light irradiation. However, the photodegradation behavior derived from light irradiation was rarely reported, comparing with the photocrosslinking. In this study, the tyramine-modified hyaluronic acid (HA/...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6873473/ https://www.ncbi.nlm.nih.gov/pubmed/31768263 http://dx.doi.org/10.1186/s40824-019-0170-1 |
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author | Hong, Bo Min Park, Su A Park, Won Ho |
author_facet | Hong, Bo Min Park, Su A Park, Won Ho |
author_sort | Hong, Bo Min |
collection | PubMed |
description | OBJECTIVES: Photocrosslinking systems of polymers have been widely studied using UV or visible light irradiation. However, the photodegradation behavior derived from light irradiation was rarely reported, comparing with the photocrosslinking. In this study, the tyramine-modified hyaluronic acid (HA/Tyr) hydrogel was prepared using riboflavin (RF) as a photoinitiator, and the degradation behavior of HA by the reactive oxygen species (ROS) generated in photochemical process was investigated. MATERIALS AND METHODS: The HA/Tyr conjugate was synthesized by EDC/NHS chemistry to introduce phenol group. Degree of substitution (DS, %) of phenol group to HA molecule was about 25%. The structural change of HA/Tyr was measured by proton nuclear magnetic resonance ((1)H-NMR) and attenuated total reflectance infrared spectroscopy (ATR-FTIR), and the rheological properties of photocrosslinked HA/Tyr hydrogel were investigated by rheometer. RESULTS: The HA/Tyr solution with 25% substitution formed a stable hydrogel via visible light irradiation in the presence of RF photoinitiator. Rheological data of HA/Tyr solution showed that the storage modulus (G’) was increased with increasing HA concentration. Additionally, it was found that RF initiated by visible light irradiation induced the degradation of HA molecular chain, and consequently reduced the viscosity of HA/Tyr solutions. CONCLUSION: The results indicate that RF-based photoinitiator system caused the degradation of HA molecule by ROS generated in photochemical process as well as the crosslinking of HA/Tyr. |
format | Online Article Text |
id | pubmed-6873473 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-68734732019-11-25 Effect of photoinitiator on chain degradation of hyaluronic acid Hong, Bo Min Park, Su A Park, Won Ho Biomater Res Research Article OBJECTIVES: Photocrosslinking systems of polymers have been widely studied using UV or visible light irradiation. However, the photodegradation behavior derived from light irradiation was rarely reported, comparing with the photocrosslinking. In this study, the tyramine-modified hyaluronic acid (HA/Tyr) hydrogel was prepared using riboflavin (RF) as a photoinitiator, and the degradation behavior of HA by the reactive oxygen species (ROS) generated in photochemical process was investigated. MATERIALS AND METHODS: The HA/Tyr conjugate was synthesized by EDC/NHS chemistry to introduce phenol group. Degree of substitution (DS, %) of phenol group to HA molecule was about 25%. The structural change of HA/Tyr was measured by proton nuclear magnetic resonance ((1)H-NMR) and attenuated total reflectance infrared spectroscopy (ATR-FTIR), and the rheological properties of photocrosslinked HA/Tyr hydrogel were investigated by rheometer. RESULTS: The HA/Tyr solution with 25% substitution formed a stable hydrogel via visible light irradiation in the presence of RF photoinitiator. Rheological data of HA/Tyr solution showed that the storage modulus (G’) was increased with increasing HA concentration. Additionally, it was found that RF initiated by visible light irradiation induced the degradation of HA molecular chain, and consequently reduced the viscosity of HA/Tyr solutions. CONCLUSION: The results indicate that RF-based photoinitiator system caused the degradation of HA molecule by ROS generated in photochemical process as well as the crosslinking of HA/Tyr. BioMed Central 2019-11-21 /pmc/articles/PMC6873473/ /pubmed/31768263 http://dx.doi.org/10.1186/s40824-019-0170-1 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Hong, Bo Min Park, Su A Park, Won Ho Effect of photoinitiator on chain degradation of hyaluronic acid |
title | Effect of photoinitiator on chain degradation of hyaluronic acid |
title_full | Effect of photoinitiator on chain degradation of hyaluronic acid |
title_fullStr | Effect of photoinitiator on chain degradation of hyaluronic acid |
title_full_unstemmed | Effect of photoinitiator on chain degradation of hyaluronic acid |
title_short | Effect of photoinitiator on chain degradation of hyaluronic acid |
title_sort | effect of photoinitiator on chain degradation of hyaluronic acid |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6873473/ https://www.ncbi.nlm.nih.gov/pubmed/31768263 http://dx.doi.org/10.1186/s40824-019-0170-1 |
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