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The factors present in regenerating muscles impact bone marrow-derived mesenchymal stromal/stem cell fusion with myoblasts
BACKGROUND: Satellite cells, a population of unipotent stem cells attached to muscle fibers, determine the excellent regenerative capability of injured skeletal muscles. Myogenic potential is also exhibited by other cell populations, which exist in the skeletal muscles or come from other niches. Mes...
Autores principales: | , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6873517/ https://www.ncbi.nlm.nih.gov/pubmed/31753006 http://dx.doi.org/10.1186/s13287-019-1444-1 |
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author | Kasprzycka, Paulina Archacka, Karolina Kowalski, Kamil Mierzejewski, Bartosz Zimowska, Małgorzata Grabowska, Iwona Piotrowski, Mariusz Rafałko, Milena Ryżko, Agata Irhashava, Aliksandra Senderowski, Kamil Gołąbek, Magdalena Stremińska, Władysława Jańczyk-Ilach, Katarzyna Koblowska, Marta Iwanicka-Nowicka, Roksana Fogtman, Anna Janowski, Mirosław Walczak, Piotr Ciemerych, Maria A. Brzoska, Edyta |
author_facet | Kasprzycka, Paulina Archacka, Karolina Kowalski, Kamil Mierzejewski, Bartosz Zimowska, Małgorzata Grabowska, Iwona Piotrowski, Mariusz Rafałko, Milena Ryżko, Agata Irhashava, Aliksandra Senderowski, Kamil Gołąbek, Magdalena Stremińska, Władysława Jańczyk-Ilach, Katarzyna Koblowska, Marta Iwanicka-Nowicka, Roksana Fogtman, Anna Janowski, Mirosław Walczak, Piotr Ciemerych, Maria A. Brzoska, Edyta |
author_sort | Kasprzycka, Paulina |
collection | PubMed |
description | BACKGROUND: Satellite cells, a population of unipotent stem cells attached to muscle fibers, determine the excellent regenerative capability of injured skeletal muscles. Myogenic potential is also exhibited by other cell populations, which exist in the skeletal muscles or come from other niches. Mesenchymal stromal/stem cells inhabiting the bone marrow do not spontaneously differentiate into muscle cells, but there is some evidence that they are capable to follow the myogenic program and/or fuse with myoblasts. METHODS: In the present study we analyzed whether IGF-1, IL-4, IL-6, and SDF-1 could impact human and porcine bone marrow-derived mesenchymal stromal/stem cells (hBM-MSCs and pBM-MSCs) and induce expression of myogenic regulatory factors, skeletal muscle-specific structural, and adhesion proteins. Moreover, we investigated whether these factors could induce both types of BM-MSCs to fuse with myoblasts. IGF-1, IL-4, IL-6, and SDF-1 were selected on the basis of their role in embryonic myogenesis as well as skeletal muscle regeneration. RESULTS: We found that hBM-MSCs and pBM-MSCs cultured in vitro in the presence of IGF-1, IL-4, IL-6, or SDF-1 did not upregulate myogenic regulatory factors. Consequently, we confirmed the lack of their naïve myogenic potential. However, we noticed that IL-4 and IL-6 impacted proliferation and IL-4, IL-6, and SDF-1 improved migration of hBM-MSCs. IL-4 treatment resulted in the significant increase in the level of mRNA encoding CD9, NCAM, VCAM, and m-cadherin, i.e., proteins engaged in cell fusion during myotube formation. Additionally, the CD9 expression level was also driven by IGF-1 treatment. Furthermore, the pre-treatment of hBM-MSCs either with IGF-1, IL-4, or SDF-1 and treatment of pBM-MSCs either with IGF-1 or IL-4 increased the efficacy of hybrid myotube formation between these cells and C2C12 myoblasts. CONCLUSIONS: To conclude, our study revealed that treatment with IGF-1, IL-4, IL-6, or SDF-1 affects BM-MSC interaction with myoblasts; however, it does not directly promote myogenic differentiation of these cells. |
format | Online Article Text |
id | pubmed-6873517 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-68735172019-12-12 The factors present in regenerating muscles impact bone marrow-derived mesenchymal stromal/stem cell fusion with myoblasts Kasprzycka, Paulina Archacka, Karolina Kowalski, Kamil Mierzejewski, Bartosz Zimowska, Małgorzata Grabowska, Iwona Piotrowski, Mariusz Rafałko, Milena Ryżko, Agata Irhashava, Aliksandra Senderowski, Kamil Gołąbek, Magdalena Stremińska, Władysława Jańczyk-Ilach, Katarzyna Koblowska, Marta Iwanicka-Nowicka, Roksana Fogtman, Anna Janowski, Mirosław Walczak, Piotr Ciemerych, Maria A. Brzoska, Edyta Stem Cell Res Ther Research BACKGROUND: Satellite cells, a population of unipotent stem cells attached to muscle fibers, determine the excellent regenerative capability of injured skeletal muscles. Myogenic potential is also exhibited by other cell populations, which exist in the skeletal muscles or come from other niches. Mesenchymal stromal/stem cells inhabiting the bone marrow do not spontaneously differentiate into muscle cells, but there is some evidence that they are capable to follow the myogenic program and/or fuse with myoblasts. METHODS: In the present study we analyzed whether IGF-1, IL-4, IL-6, and SDF-1 could impact human and porcine bone marrow-derived mesenchymal stromal/stem cells (hBM-MSCs and pBM-MSCs) and induce expression of myogenic regulatory factors, skeletal muscle-specific structural, and adhesion proteins. Moreover, we investigated whether these factors could induce both types of BM-MSCs to fuse with myoblasts. IGF-1, IL-4, IL-6, and SDF-1 were selected on the basis of their role in embryonic myogenesis as well as skeletal muscle regeneration. RESULTS: We found that hBM-MSCs and pBM-MSCs cultured in vitro in the presence of IGF-1, IL-4, IL-6, or SDF-1 did not upregulate myogenic regulatory factors. Consequently, we confirmed the lack of their naïve myogenic potential. However, we noticed that IL-4 and IL-6 impacted proliferation and IL-4, IL-6, and SDF-1 improved migration of hBM-MSCs. IL-4 treatment resulted in the significant increase in the level of mRNA encoding CD9, NCAM, VCAM, and m-cadherin, i.e., proteins engaged in cell fusion during myotube formation. Additionally, the CD9 expression level was also driven by IGF-1 treatment. Furthermore, the pre-treatment of hBM-MSCs either with IGF-1, IL-4, or SDF-1 and treatment of pBM-MSCs either with IGF-1 or IL-4 increased the efficacy of hybrid myotube formation between these cells and C2C12 myoblasts. CONCLUSIONS: To conclude, our study revealed that treatment with IGF-1, IL-4, IL-6, or SDF-1 affects BM-MSC interaction with myoblasts; however, it does not directly promote myogenic differentiation of these cells. BioMed Central 2019-11-21 /pmc/articles/PMC6873517/ /pubmed/31753006 http://dx.doi.org/10.1186/s13287-019-1444-1 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Kasprzycka, Paulina Archacka, Karolina Kowalski, Kamil Mierzejewski, Bartosz Zimowska, Małgorzata Grabowska, Iwona Piotrowski, Mariusz Rafałko, Milena Ryżko, Agata Irhashava, Aliksandra Senderowski, Kamil Gołąbek, Magdalena Stremińska, Władysława Jańczyk-Ilach, Katarzyna Koblowska, Marta Iwanicka-Nowicka, Roksana Fogtman, Anna Janowski, Mirosław Walczak, Piotr Ciemerych, Maria A. Brzoska, Edyta The factors present in regenerating muscles impact bone marrow-derived mesenchymal stromal/stem cell fusion with myoblasts |
title | The factors present in regenerating muscles impact bone marrow-derived mesenchymal stromal/stem cell fusion with myoblasts |
title_full | The factors present in regenerating muscles impact bone marrow-derived mesenchymal stromal/stem cell fusion with myoblasts |
title_fullStr | The factors present in regenerating muscles impact bone marrow-derived mesenchymal stromal/stem cell fusion with myoblasts |
title_full_unstemmed | The factors present in regenerating muscles impact bone marrow-derived mesenchymal stromal/stem cell fusion with myoblasts |
title_short | The factors present in regenerating muscles impact bone marrow-derived mesenchymal stromal/stem cell fusion with myoblasts |
title_sort | factors present in regenerating muscles impact bone marrow-derived mesenchymal stromal/stem cell fusion with myoblasts |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6873517/ https://www.ncbi.nlm.nih.gov/pubmed/31753006 http://dx.doi.org/10.1186/s13287-019-1444-1 |
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