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Cytotoxic activity of crude extracts from Datura stramonium’s fungal endophytes against A549 lung carcinoma and UMG87 glioblastoma cell lines and LC-QTOF-MS/MS based metabolite profiling

BACKGROUND: Endophytic fungi are a proven source of bioactive secondary metabolites that may provide lead compounds for novel drug discovery. In this study, crude extracts from fungal endophytes isolated from Datura stramonium were evaluated for cytotoxic activity on two human cancer cell lines. MET...

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Autores principales: Tapfuma, Kudzanai Ian, Uche-Okereafor, Nkemdinma, Sebola, Tendani Edith, Hussan, Raeesa, Mekuto, Lukhanyo, Makatini, Maya Mellisa, Green, Ezekiel, Mavumengwana, Vuyo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6873518/
https://www.ncbi.nlm.nih.gov/pubmed/31752824
http://dx.doi.org/10.1186/s12906-019-2752-9
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author Tapfuma, Kudzanai Ian
Uche-Okereafor, Nkemdinma
Sebola, Tendani Edith
Hussan, Raeesa
Mekuto, Lukhanyo
Makatini, Maya Mellisa
Green, Ezekiel
Mavumengwana, Vuyo
author_facet Tapfuma, Kudzanai Ian
Uche-Okereafor, Nkemdinma
Sebola, Tendani Edith
Hussan, Raeesa
Mekuto, Lukhanyo
Makatini, Maya Mellisa
Green, Ezekiel
Mavumengwana, Vuyo
author_sort Tapfuma, Kudzanai Ian
collection PubMed
description BACKGROUND: Endophytic fungi are a proven source of bioactive secondary metabolites that may provide lead compounds for novel drug discovery. In this study, crude extracts from fungal endophytes isolated from Datura stramonium were evaluated for cytotoxic activity on two human cancer cell lines. METHODS: Fungal endophytes were isolated from surface sterilized aerial parts of D. stramonium and identified using molecular, morphological and phylogenetic methods. Ethyl acetate crude extracts from these isolates were evaluated for cytotoxic activity on A549 lung carcinoma and UMG87 glioblastoma cell lines. Metabolite profiling was then performed by liquid chromatography coupled to quadrupole time-of-flight with tandem mass spectrometry (LC-QTOF-MS/MS) for the cytotoxic crude extract. RESULTS: Eleven fungal endophytes were identified from D. stramonium. Significant cytotoxicity was only observed from the crude extract of Alternaria sp. KTDL7 on UMG87 glioblastoma cells (IC(50) = 21.49 μg/ml). Metabolite profiling of this crude extract tentatively revealed the presence of the following secondary metabolites: 1,8-dihydroxynaphthalene (1), anserinone B (2), phelligridin B (3), metacytofilin (4), phomopsidin (5) and vermixocin A (6). Compounds 2 and 3 have been shown to be cytotoxic in literature. CONCLUSION: The findings in this study suggest that the crude extract of Alternaria sp. KTDL7 possesses compound(s) cytotoxic to glioblastoma multiforme cells. Future studies to isolate and characterize the cytotoxic compound(s) from this fungus could result in lead development of a fungal-based drug for glioblastoma multiforme treatment.
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spelling pubmed-68735182019-12-12 Cytotoxic activity of crude extracts from Datura stramonium’s fungal endophytes against A549 lung carcinoma and UMG87 glioblastoma cell lines and LC-QTOF-MS/MS based metabolite profiling Tapfuma, Kudzanai Ian Uche-Okereafor, Nkemdinma Sebola, Tendani Edith Hussan, Raeesa Mekuto, Lukhanyo Makatini, Maya Mellisa Green, Ezekiel Mavumengwana, Vuyo BMC Complement Altern Med Research Article BACKGROUND: Endophytic fungi are a proven source of bioactive secondary metabolites that may provide lead compounds for novel drug discovery. In this study, crude extracts from fungal endophytes isolated from Datura stramonium were evaluated for cytotoxic activity on two human cancer cell lines. METHODS: Fungal endophytes were isolated from surface sterilized aerial parts of D. stramonium and identified using molecular, morphological and phylogenetic methods. Ethyl acetate crude extracts from these isolates were evaluated for cytotoxic activity on A549 lung carcinoma and UMG87 glioblastoma cell lines. Metabolite profiling was then performed by liquid chromatography coupled to quadrupole time-of-flight with tandem mass spectrometry (LC-QTOF-MS/MS) for the cytotoxic crude extract. RESULTS: Eleven fungal endophytes were identified from D. stramonium. Significant cytotoxicity was only observed from the crude extract of Alternaria sp. KTDL7 on UMG87 glioblastoma cells (IC(50) = 21.49 μg/ml). Metabolite profiling of this crude extract tentatively revealed the presence of the following secondary metabolites: 1,8-dihydroxynaphthalene (1), anserinone B (2), phelligridin B (3), metacytofilin (4), phomopsidin (5) and vermixocin A (6). Compounds 2 and 3 have been shown to be cytotoxic in literature. CONCLUSION: The findings in this study suggest that the crude extract of Alternaria sp. KTDL7 possesses compound(s) cytotoxic to glioblastoma multiforme cells. Future studies to isolate and characterize the cytotoxic compound(s) from this fungus could result in lead development of a fungal-based drug for glioblastoma multiforme treatment. BioMed Central 2019-11-21 /pmc/articles/PMC6873518/ /pubmed/31752824 http://dx.doi.org/10.1186/s12906-019-2752-9 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Tapfuma, Kudzanai Ian
Uche-Okereafor, Nkemdinma
Sebola, Tendani Edith
Hussan, Raeesa
Mekuto, Lukhanyo
Makatini, Maya Mellisa
Green, Ezekiel
Mavumengwana, Vuyo
Cytotoxic activity of crude extracts from Datura stramonium’s fungal endophytes against A549 lung carcinoma and UMG87 glioblastoma cell lines and LC-QTOF-MS/MS based metabolite profiling
title Cytotoxic activity of crude extracts from Datura stramonium’s fungal endophytes against A549 lung carcinoma and UMG87 glioblastoma cell lines and LC-QTOF-MS/MS based metabolite profiling
title_full Cytotoxic activity of crude extracts from Datura stramonium’s fungal endophytes against A549 lung carcinoma and UMG87 glioblastoma cell lines and LC-QTOF-MS/MS based metabolite profiling
title_fullStr Cytotoxic activity of crude extracts from Datura stramonium’s fungal endophytes against A549 lung carcinoma and UMG87 glioblastoma cell lines and LC-QTOF-MS/MS based metabolite profiling
title_full_unstemmed Cytotoxic activity of crude extracts from Datura stramonium’s fungal endophytes against A549 lung carcinoma and UMG87 glioblastoma cell lines and LC-QTOF-MS/MS based metabolite profiling
title_short Cytotoxic activity of crude extracts from Datura stramonium’s fungal endophytes against A549 lung carcinoma and UMG87 glioblastoma cell lines and LC-QTOF-MS/MS based metabolite profiling
title_sort cytotoxic activity of crude extracts from datura stramonium’s fungal endophytes against a549 lung carcinoma and umg87 glioblastoma cell lines and lc-qtof-ms/ms based metabolite profiling
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6873518/
https://www.ncbi.nlm.nih.gov/pubmed/31752824
http://dx.doi.org/10.1186/s12906-019-2752-9
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