Cargando…
Regulatory effect of Garidisan on dysbiosis of the gut microbiota in the mouse model of ulcerative colitis induced by dextran sulfate sodium
BACKGROUND: Ulcerative colitis (UC) is a modern refractory disease, and its etiology has been difficult to discern. Studies have shown that UC is closely associated with the gut microbiota. Garidisan is composed of wild poppy and Artemisia frigida Willd and is commonly used for the treatment of UC i...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6873523/ https://www.ncbi.nlm.nih.gov/pubmed/31752807 http://dx.doi.org/10.1186/s12906-019-2750-y |
_version_ | 1783472678467796992 |
---|---|
author | Pei, Ling-yan Ke, Yu-shi Zhao, Huan-hu Liu, Wei-zhi Wang, Lin Jia, Chao Shi, Meng-ni Fu, Qian-hui Cui, Jian Li, Shu-chun |
author_facet | Pei, Ling-yan Ke, Yu-shi Zhao, Huan-hu Liu, Wei-zhi Wang, Lin Jia, Chao Shi, Meng-ni Fu, Qian-hui Cui, Jian Li, Shu-chun |
author_sort | Pei, Ling-yan |
collection | PubMed |
description | BACKGROUND: Ulcerative colitis (UC) is a modern refractory disease, and its etiology has been difficult to discern. Studies have shown that UC is closely associated with the gut microbiota. Garidisan is composed of wild poppy and Artemisia frigida Willd and is commonly used for the treatment of UC in Inner Mongolia, China. In clinical settings, Garidisan has been found to treat UC effectively, with low recurrence. Previous studies have shown that Garidisan has a good therapeutic effect on mice with UC, but the therapeutic mechanism is still unclear. In this study, we investigated the regulatory effect of Garidisan on dysbiosis of the gut microbiota in a UC mouse model and explored the possible mechanism of the therapeutic effect of Garidisan on UC. METHODS: The UC mouse model was established by the dextran sulfate sodium (DSS) circulating free water drinking method, and the luminal contents were sampled under sterile conditions. High-throughput sequencing of the 16S rRNA gene V3 + V4 region of the luminal contents of the control group, model group, and Garidisan group was conducted, and clustering of operational taxonomic units (OTUs) and species annotation were performed. The differences in species composition and microbial community structure between individual groups of samples were analyzed using MetaStat, LefSe, rank sum test, and Bayesian causal network analysis. RESULTS: The UC mouse model was successfully established and the sequencing results were of adequate quality. There were significant differences in the diversity of luminal contents between the control group, model group, and Garidisan group, and the differences between groups were greater than those within any group. The therapeutic effect of Garidisan on UC is attributed to the direct effect on the Lachnospiraceae family of bacteria. CONCLUSION: Garidisan has a good regulatory effect on the gut microbiota, and Lachnospiraceae could be an important direct target of Garidisan for the treatment of UC. |
format | Online Article Text |
id | pubmed-6873523 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-68735232019-12-12 Regulatory effect of Garidisan on dysbiosis of the gut microbiota in the mouse model of ulcerative colitis induced by dextran sulfate sodium Pei, Ling-yan Ke, Yu-shi Zhao, Huan-hu Liu, Wei-zhi Wang, Lin Jia, Chao Shi, Meng-ni Fu, Qian-hui Cui, Jian Li, Shu-chun BMC Complement Altern Med Research Article BACKGROUND: Ulcerative colitis (UC) is a modern refractory disease, and its etiology has been difficult to discern. Studies have shown that UC is closely associated with the gut microbiota. Garidisan is composed of wild poppy and Artemisia frigida Willd and is commonly used for the treatment of UC in Inner Mongolia, China. In clinical settings, Garidisan has been found to treat UC effectively, with low recurrence. Previous studies have shown that Garidisan has a good therapeutic effect on mice with UC, but the therapeutic mechanism is still unclear. In this study, we investigated the regulatory effect of Garidisan on dysbiosis of the gut microbiota in a UC mouse model and explored the possible mechanism of the therapeutic effect of Garidisan on UC. METHODS: The UC mouse model was established by the dextran sulfate sodium (DSS) circulating free water drinking method, and the luminal contents were sampled under sterile conditions. High-throughput sequencing of the 16S rRNA gene V3 + V4 region of the luminal contents of the control group, model group, and Garidisan group was conducted, and clustering of operational taxonomic units (OTUs) and species annotation were performed. The differences in species composition and microbial community structure between individual groups of samples were analyzed using MetaStat, LefSe, rank sum test, and Bayesian causal network analysis. RESULTS: The UC mouse model was successfully established and the sequencing results were of adequate quality. There were significant differences in the diversity of luminal contents between the control group, model group, and Garidisan group, and the differences between groups were greater than those within any group. The therapeutic effect of Garidisan on UC is attributed to the direct effect on the Lachnospiraceae family of bacteria. CONCLUSION: Garidisan has a good regulatory effect on the gut microbiota, and Lachnospiraceae could be an important direct target of Garidisan for the treatment of UC. BioMed Central 2019-11-21 /pmc/articles/PMC6873523/ /pubmed/31752807 http://dx.doi.org/10.1186/s12906-019-2750-y Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Pei, Ling-yan Ke, Yu-shi Zhao, Huan-hu Liu, Wei-zhi Wang, Lin Jia, Chao Shi, Meng-ni Fu, Qian-hui Cui, Jian Li, Shu-chun Regulatory effect of Garidisan on dysbiosis of the gut microbiota in the mouse model of ulcerative colitis induced by dextran sulfate sodium |
title | Regulatory effect of Garidisan on dysbiosis of the gut microbiota in the mouse model of ulcerative colitis induced by dextran sulfate sodium |
title_full | Regulatory effect of Garidisan on dysbiosis of the gut microbiota in the mouse model of ulcerative colitis induced by dextran sulfate sodium |
title_fullStr | Regulatory effect of Garidisan on dysbiosis of the gut microbiota in the mouse model of ulcerative colitis induced by dextran sulfate sodium |
title_full_unstemmed | Regulatory effect of Garidisan on dysbiosis of the gut microbiota in the mouse model of ulcerative colitis induced by dextran sulfate sodium |
title_short | Regulatory effect of Garidisan on dysbiosis of the gut microbiota in the mouse model of ulcerative colitis induced by dextran sulfate sodium |
title_sort | regulatory effect of garidisan on dysbiosis of the gut microbiota in the mouse model of ulcerative colitis induced by dextran sulfate sodium |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6873523/ https://www.ncbi.nlm.nih.gov/pubmed/31752807 http://dx.doi.org/10.1186/s12906-019-2750-y |
work_keys_str_mv | AT peilingyan regulatoryeffectofgaridisanondysbiosisofthegutmicrobiotainthemousemodelofulcerativecolitisinducedbydextransulfatesodium AT keyushi regulatoryeffectofgaridisanondysbiosisofthegutmicrobiotainthemousemodelofulcerativecolitisinducedbydextransulfatesodium AT zhaohuanhu regulatoryeffectofgaridisanondysbiosisofthegutmicrobiotainthemousemodelofulcerativecolitisinducedbydextransulfatesodium AT liuweizhi regulatoryeffectofgaridisanondysbiosisofthegutmicrobiotainthemousemodelofulcerativecolitisinducedbydextransulfatesodium AT wanglin regulatoryeffectofgaridisanondysbiosisofthegutmicrobiotainthemousemodelofulcerativecolitisinducedbydextransulfatesodium AT jiachao regulatoryeffectofgaridisanondysbiosisofthegutmicrobiotainthemousemodelofulcerativecolitisinducedbydextransulfatesodium AT shimengni regulatoryeffectofgaridisanondysbiosisofthegutmicrobiotainthemousemodelofulcerativecolitisinducedbydextransulfatesodium AT fuqianhui regulatoryeffectofgaridisanondysbiosisofthegutmicrobiotainthemousemodelofulcerativecolitisinducedbydextransulfatesodium AT cuijian regulatoryeffectofgaridisanondysbiosisofthegutmicrobiotainthemousemodelofulcerativecolitisinducedbydextransulfatesodium AT lishuchun regulatoryeffectofgaridisanondysbiosisofthegutmicrobiotainthemousemodelofulcerativecolitisinducedbydextransulfatesodium |