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Comparison of four commercially available ELISA kits for diagnosis of Fasciola hepatica in Irish cattle
BACKGROUND: Fasciola hepatica is a liver parasite of mammals and it results in poor welfare outcomes and economic losses in ruminants. While faecal egg count is the test most commonly used for diagnosis, it does not indicate presence of migrating immature stages. Serological techniques increase sens...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6873750/ https://www.ncbi.nlm.nih.gov/pubmed/31752880 http://dx.doi.org/10.1186/s12917-019-2160-x |
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author | Munita, Maria Pia Rea, Rosemary Martinez-Ibeas, Ana Maria Byrne, Noel Kennedy, Aideen Sekiya, Mary Mulcahy, Grace Sayers, Riona |
author_facet | Munita, Maria Pia Rea, Rosemary Martinez-Ibeas, Ana Maria Byrne, Noel Kennedy, Aideen Sekiya, Mary Mulcahy, Grace Sayers, Riona |
author_sort | Munita, Maria Pia |
collection | PubMed |
description | BACKGROUND: Fasciola hepatica is a liver parasite of mammals and it results in poor welfare outcomes and economic losses in ruminants. While faecal egg count is the test most commonly used for diagnosis, it does not indicate presence of migrating immature stages. Serological techniques increase sensitivity at all stages of the liver fluke infection. The aim of this study was to compare four commercially available ELISA tests for the diagnosis of F. hepatica. For this purpose, we tested three sample types; (i) known F. hepatica status sera from an experimental infection for the comparison of sensitivities and specificities, (ii) sera from pre- and post-flukicide-treated (albendazole, closantel, nitroxynil and triclabendazole) beef cattle to contrast the differences of seropositivity before and after treatment, and (iii) bulk tank milk samples from dairy herds sampled during high and low F. hepatica exposure periods for assessing seasonal variations with the four tests available. Samples were tested using ELISA kits supplied by four manufacturers (Ildana Biotech, IDEXX, Svanova, and Bio-X). Samples were analysed simultaneously and in duplicate. RESULTS: In the control population Ildana, IDEXX and Bio-X presented 100% sensitivity (Se) and specificity (Sp), Svanovir presented a Se of 59% and a Sp of 96%. In flukicide-treated beef cattle, kits highlighted decreasing antibody levels 90 days post-treatment in variable degrees. Finally, bulk milk showed a significant decrease in ELISA value between high and low fluke exposure periods with all tests studied. CONCLUSIONS: Se and Sp found in the present study, confirm that Ildana, IDEXX and Bio-X are accurate for the detection of F. hepatica exposure in Irish cattle. Svanovir Se and Sp in this population, indicate that a larger study is necessary to confirm this test characteristic in Irish herds. In post-treatment use, Bio-X showed a consistent and significant decrease of ELISA value in all groups treated, denoting to be a reliable tool for assessing treatment effect at 90 days post-treatment. Finally, all tests showed to be a reliable tool for the F. hepatica monitoring of high and low exposure seasons, using bulk tank milk samples. |
format | Online Article Text |
id | pubmed-6873750 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-68737502019-11-25 Comparison of four commercially available ELISA kits for diagnosis of Fasciola hepatica in Irish cattle Munita, Maria Pia Rea, Rosemary Martinez-Ibeas, Ana Maria Byrne, Noel Kennedy, Aideen Sekiya, Mary Mulcahy, Grace Sayers, Riona BMC Vet Res Research Article BACKGROUND: Fasciola hepatica is a liver parasite of mammals and it results in poor welfare outcomes and economic losses in ruminants. While faecal egg count is the test most commonly used for diagnosis, it does not indicate presence of migrating immature stages. Serological techniques increase sensitivity at all stages of the liver fluke infection. The aim of this study was to compare four commercially available ELISA tests for the diagnosis of F. hepatica. For this purpose, we tested three sample types; (i) known F. hepatica status sera from an experimental infection for the comparison of sensitivities and specificities, (ii) sera from pre- and post-flukicide-treated (albendazole, closantel, nitroxynil and triclabendazole) beef cattle to contrast the differences of seropositivity before and after treatment, and (iii) bulk tank milk samples from dairy herds sampled during high and low F. hepatica exposure periods for assessing seasonal variations with the four tests available. Samples were tested using ELISA kits supplied by four manufacturers (Ildana Biotech, IDEXX, Svanova, and Bio-X). Samples were analysed simultaneously and in duplicate. RESULTS: In the control population Ildana, IDEXX and Bio-X presented 100% sensitivity (Se) and specificity (Sp), Svanovir presented a Se of 59% and a Sp of 96%. In flukicide-treated beef cattle, kits highlighted decreasing antibody levels 90 days post-treatment in variable degrees. Finally, bulk milk showed a significant decrease in ELISA value between high and low fluke exposure periods with all tests studied. CONCLUSIONS: Se and Sp found in the present study, confirm that Ildana, IDEXX and Bio-X are accurate for the detection of F. hepatica exposure in Irish cattle. Svanovir Se and Sp in this population, indicate that a larger study is necessary to confirm this test characteristic in Irish herds. In post-treatment use, Bio-X showed a consistent and significant decrease of ELISA value in all groups treated, denoting to be a reliable tool for assessing treatment effect at 90 days post-treatment. Finally, all tests showed to be a reliable tool for the F. hepatica monitoring of high and low exposure seasons, using bulk tank milk samples. BioMed Central 2019-11-21 /pmc/articles/PMC6873750/ /pubmed/31752880 http://dx.doi.org/10.1186/s12917-019-2160-x Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Munita, Maria Pia Rea, Rosemary Martinez-Ibeas, Ana Maria Byrne, Noel Kennedy, Aideen Sekiya, Mary Mulcahy, Grace Sayers, Riona Comparison of four commercially available ELISA kits for diagnosis of Fasciola hepatica in Irish cattle |
title | Comparison of four commercially available ELISA kits for diagnosis of Fasciola hepatica in Irish cattle |
title_full | Comparison of four commercially available ELISA kits for diagnosis of Fasciola hepatica in Irish cattle |
title_fullStr | Comparison of four commercially available ELISA kits for diagnosis of Fasciola hepatica in Irish cattle |
title_full_unstemmed | Comparison of four commercially available ELISA kits for diagnosis of Fasciola hepatica in Irish cattle |
title_short | Comparison of four commercially available ELISA kits for diagnosis of Fasciola hepatica in Irish cattle |
title_sort | comparison of four commercially available elisa kits for diagnosis of fasciola hepatica in irish cattle |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6873750/ https://www.ncbi.nlm.nih.gov/pubmed/31752880 http://dx.doi.org/10.1186/s12917-019-2160-x |
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