Cargando…

TNIP1 Inhibits Proliferation And Promotes Apoptosis In Clear Cell Renal Carcinoma Through Targeting C/Ebpβ

BACKGROUND/PURPOSE: TNF-α-induced protein 3-interacting protein 1 (TNIP1) is active in various cancers, but its expression and function in renal cell carcinoma (RCC) have not been described. This study investigated the role of TNIP1 in clear cell renal cell carcinomas (ccRCC), which accounts for 75–...

Descripción completa

Detalles Bibliográficos
Autores principales: Yang, Yong, Fan, Jinhai, Han, Shenglu, Li, Enyuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6874165/
https://www.ncbi.nlm.nih.gov/pubmed/31819484
http://dx.doi.org/10.2147/OTT.S216138
_version_ 1783472789104099328
author Yang, Yong
Fan, Jinhai
Han, Shenglu
Li, Enyuan
author_facet Yang, Yong
Fan, Jinhai
Han, Shenglu
Li, Enyuan
author_sort Yang, Yong
collection PubMed
description BACKGROUND/PURPOSE: TNF-α-induced protein 3-interacting protein 1 (TNIP1) is active in various cancers, but its expression and function in renal cell carcinoma (RCC) have not been described. This study investigated the role of TNIP1 in clear cell renal cell carcinomas (ccRCC), which accounts for 75–80% of RCC and has a poor prognosis. METHODS: The expression of TNIP1 in human ccRCC tissues and cells was detected by real-time quantitative reverse transcription–polymerase chain reaction (qRT-PCR), Western blot (WB), and immunohistochemical (IHC) staining. Cell proliferation was assayed by a cell counting kit (CCK)-8 assay; cell cycle analysis and apoptosis assay were done by flow cytometry. RESULTS: TNIP1 is downregulated in both ccRCC human tissues and cells. Besides, TNIP1 downregulation promoted cell proliferation with more cell cycle entry, and inhibited apoptosis. TNIP1 downregulation was associated with increased of expression of the Bcl-2 anti-apoptosis gene and decreased expression of the Bax apoptosis-promoting gene and cleaved-caspase-3 by negatively regulating C/EBPβ expression. CONCLUSION: TNIP1 acted as a tumor-inhibitor in ccRCC by targeting C/EBPβ. The results warrant study of TNIP1 as a potential diagnostic marker and therapeutic target of ccRCC.
format Online
Article
Text
id pubmed-6874165
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Dove
record_format MEDLINE/PubMed
spelling pubmed-68741652019-12-09 TNIP1 Inhibits Proliferation And Promotes Apoptosis In Clear Cell Renal Carcinoma Through Targeting C/Ebpβ Yang, Yong Fan, Jinhai Han, Shenglu Li, Enyuan Onco Targets Ther Original Research BACKGROUND/PURPOSE: TNF-α-induced protein 3-interacting protein 1 (TNIP1) is active in various cancers, but its expression and function in renal cell carcinoma (RCC) have not been described. This study investigated the role of TNIP1 in clear cell renal cell carcinomas (ccRCC), which accounts for 75–80% of RCC and has a poor prognosis. METHODS: The expression of TNIP1 in human ccRCC tissues and cells was detected by real-time quantitative reverse transcription–polymerase chain reaction (qRT-PCR), Western blot (WB), and immunohistochemical (IHC) staining. Cell proliferation was assayed by a cell counting kit (CCK)-8 assay; cell cycle analysis and apoptosis assay were done by flow cytometry. RESULTS: TNIP1 is downregulated in both ccRCC human tissues and cells. Besides, TNIP1 downregulation promoted cell proliferation with more cell cycle entry, and inhibited apoptosis. TNIP1 downregulation was associated with increased of expression of the Bcl-2 anti-apoptosis gene and decreased expression of the Bax apoptosis-promoting gene and cleaved-caspase-3 by negatively regulating C/EBPβ expression. CONCLUSION: TNIP1 acted as a tumor-inhibitor in ccRCC by targeting C/EBPβ. The results warrant study of TNIP1 as a potential diagnostic marker and therapeutic target of ccRCC. Dove 2019-11-18 /pmc/articles/PMC6874165/ /pubmed/31819484 http://dx.doi.org/10.2147/OTT.S216138 Text en © 2019 Yang et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Yang, Yong
Fan, Jinhai
Han, Shenglu
Li, Enyuan
TNIP1 Inhibits Proliferation And Promotes Apoptosis In Clear Cell Renal Carcinoma Through Targeting C/Ebpβ
title TNIP1 Inhibits Proliferation And Promotes Apoptosis In Clear Cell Renal Carcinoma Through Targeting C/Ebpβ
title_full TNIP1 Inhibits Proliferation And Promotes Apoptosis In Clear Cell Renal Carcinoma Through Targeting C/Ebpβ
title_fullStr TNIP1 Inhibits Proliferation And Promotes Apoptosis In Clear Cell Renal Carcinoma Through Targeting C/Ebpβ
title_full_unstemmed TNIP1 Inhibits Proliferation And Promotes Apoptosis In Clear Cell Renal Carcinoma Through Targeting C/Ebpβ
title_short TNIP1 Inhibits Proliferation And Promotes Apoptosis In Clear Cell Renal Carcinoma Through Targeting C/Ebpβ
title_sort tnip1 inhibits proliferation and promotes apoptosis in clear cell renal carcinoma through targeting c/ebpβ
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6874165/
https://www.ncbi.nlm.nih.gov/pubmed/31819484
http://dx.doi.org/10.2147/OTT.S216138
work_keys_str_mv AT yangyong tnip1inhibitsproliferationandpromotesapoptosisinclearcellrenalcarcinomathroughtargetingcebpb
AT fanjinhai tnip1inhibitsproliferationandpromotesapoptosisinclearcellrenalcarcinomathroughtargetingcebpb
AT hanshenglu tnip1inhibitsproliferationandpromotesapoptosisinclearcellrenalcarcinomathroughtargetingcebpb
AT lienyuan tnip1inhibitsproliferationandpromotesapoptosisinclearcellrenalcarcinomathroughtargetingcebpb