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Blood Particle Separation Using Dielectrophoresis in A Novel Microchannel: A Numerical Study

OBJECTIVE: We present a four-branch model of the dielectrophoresis (DEP) method that takes into consideration the inherent properties of particles, including size, electrical conductivity, and permittivity coefficient. By using this model, bioparticles can be continuously separated by the applicatio...

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Autores principales: Zahedi Siani, Omid, Sojoodi, Mahdi, Zabetian Targhi, Mohammad, Movahedin, Mansoureh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royan Institute 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6874797/
https://www.ncbi.nlm.nih.gov/pubmed/31721537
http://dx.doi.org/10.22074/cellj.2020.6386
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author Zahedi Siani, Omid
Sojoodi, Mahdi
Zabetian Targhi, Mohammad
Movahedin, Mansoureh
author_facet Zahedi Siani, Omid
Sojoodi, Mahdi
Zabetian Targhi, Mohammad
Movahedin, Mansoureh
author_sort Zahedi Siani, Omid
collection PubMed
description OBJECTIVE: We present a four-branch model of the dielectrophoresis (DEP) method that takes into consideration the inherent properties of particles, including size, electrical conductivity, and permittivity coefficient. By using this model, bioparticles can be continuously separated by the application of only a one-stage separation process. MATERIALS AND METHODS: In this numerical study, we based the separation process on the differences in the particle sizes. We used the various negative DEP forces on the particles caused by the electrodes to separate them with a high efficiency. The particle separator could separate blood cells because of their different sizes. RESULTS: Blood cells greater than 12 μm were guided to a special branch, which improved separation efficiency because it prevented the deposition of particles in other branches. The designed device had the capability to separate blood cells with diameters of 2.0 μm, 6.2 μm, 10.0 μm, and greater than 12.0 μm. The applied voltage to the electrodes was 50 V with a frequency of 100 kHz. CONCLUSION: The proposed device is a simple, efficient DEP-based continuous cell separator. This capability makes it ideal for use in various biomedical applications, including cell therapy and cell separation, and results in a throughput increment of microfluidics devices.
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spelling pubmed-68747972020-07-01 Blood Particle Separation Using Dielectrophoresis in A Novel Microchannel: A Numerical Study Zahedi Siani, Omid Sojoodi, Mahdi Zabetian Targhi, Mohammad Movahedin, Mansoureh Cell J Original Article OBJECTIVE: We present a four-branch model of the dielectrophoresis (DEP) method that takes into consideration the inherent properties of particles, including size, electrical conductivity, and permittivity coefficient. By using this model, bioparticles can be continuously separated by the application of only a one-stage separation process. MATERIALS AND METHODS: In this numerical study, we based the separation process on the differences in the particle sizes. We used the various negative DEP forces on the particles caused by the electrodes to separate them with a high efficiency. The particle separator could separate blood cells because of their different sizes. RESULTS: Blood cells greater than 12 μm were guided to a special branch, which improved separation efficiency because it prevented the deposition of particles in other branches. The designed device had the capability to separate blood cells with diameters of 2.0 μm, 6.2 μm, 10.0 μm, and greater than 12.0 μm. The applied voltage to the electrodes was 50 V with a frequency of 100 kHz. CONCLUSION: The proposed device is a simple, efficient DEP-based continuous cell separator. This capability makes it ideal for use in various biomedical applications, including cell therapy and cell separation, and results in a throughput increment of microfluidics devices. Royan Institute 2020 2019-10-14 /pmc/articles/PMC6874797/ /pubmed/31721537 http://dx.doi.org/10.22074/cellj.2020.6386 Text en The Cell Journal (Yakhteh) is an open access journal which means the articles are freely available online for any individual author to download and use the providing address. The journal is licensed under a Creative Commons Attribution-Non Commercial 3.0 Unported License which allows the author(s) to hold the copyright without restrictions that is permitting unrestricted use, distribution, and reproduction in any medium provided the original work is properly cited. http://creativecommons.org/licenses/by/3/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Zahedi Siani, Omid
Sojoodi, Mahdi
Zabetian Targhi, Mohammad
Movahedin, Mansoureh
Blood Particle Separation Using Dielectrophoresis in A Novel Microchannel: A Numerical Study
title Blood Particle Separation Using Dielectrophoresis in A Novel Microchannel: A Numerical Study
title_full Blood Particle Separation Using Dielectrophoresis in A Novel Microchannel: A Numerical Study
title_fullStr Blood Particle Separation Using Dielectrophoresis in A Novel Microchannel: A Numerical Study
title_full_unstemmed Blood Particle Separation Using Dielectrophoresis in A Novel Microchannel: A Numerical Study
title_short Blood Particle Separation Using Dielectrophoresis in A Novel Microchannel: A Numerical Study
title_sort blood particle separation using dielectrophoresis in a novel microchannel: a numerical study
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6874797/
https://www.ncbi.nlm.nih.gov/pubmed/31721537
http://dx.doi.org/10.22074/cellj.2020.6386
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