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Blood Particle Separation Using Dielectrophoresis in A Novel Microchannel: A Numerical Study
OBJECTIVE: We present a four-branch model of the dielectrophoresis (DEP) method that takes into consideration the inherent properties of particles, including size, electrical conductivity, and permittivity coefficient. By using this model, bioparticles can be continuously separated by the applicatio...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Royan Institute
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6874797/ https://www.ncbi.nlm.nih.gov/pubmed/31721537 http://dx.doi.org/10.22074/cellj.2020.6386 |
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author | Zahedi Siani, Omid Sojoodi, Mahdi Zabetian Targhi, Mohammad Movahedin, Mansoureh |
author_facet | Zahedi Siani, Omid Sojoodi, Mahdi Zabetian Targhi, Mohammad Movahedin, Mansoureh |
author_sort | Zahedi Siani, Omid |
collection | PubMed |
description | OBJECTIVE: We present a four-branch model of the dielectrophoresis (DEP) method that takes into consideration the inherent properties of particles, including size, electrical conductivity, and permittivity coefficient. By using this model, bioparticles can be continuously separated by the application of only a one-stage separation process. MATERIALS AND METHODS: In this numerical study, we based the separation process on the differences in the particle sizes. We used the various negative DEP forces on the particles caused by the electrodes to separate them with a high efficiency. The particle separator could separate blood cells because of their different sizes. RESULTS: Blood cells greater than 12 μm were guided to a special branch, which improved separation efficiency because it prevented the deposition of particles in other branches. The designed device had the capability to separate blood cells with diameters of 2.0 μm, 6.2 μm, 10.0 μm, and greater than 12.0 μm. The applied voltage to the electrodes was 50 V with a frequency of 100 kHz. CONCLUSION: The proposed device is a simple, efficient DEP-based continuous cell separator. This capability makes it ideal for use in various biomedical applications, including cell therapy and cell separation, and results in a throughput increment of microfluidics devices. |
format | Online Article Text |
id | pubmed-6874797 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Royan Institute |
record_format | MEDLINE/PubMed |
spelling | pubmed-68747972020-07-01 Blood Particle Separation Using Dielectrophoresis in A Novel Microchannel: A Numerical Study Zahedi Siani, Omid Sojoodi, Mahdi Zabetian Targhi, Mohammad Movahedin, Mansoureh Cell J Original Article OBJECTIVE: We present a four-branch model of the dielectrophoresis (DEP) method that takes into consideration the inherent properties of particles, including size, electrical conductivity, and permittivity coefficient. By using this model, bioparticles can be continuously separated by the application of only a one-stage separation process. MATERIALS AND METHODS: In this numerical study, we based the separation process on the differences in the particle sizes. We used the various negative DEP forces on the particles caused by the electrodes to separate them with a high efficiency. The particle separator could separate blood cells because of their different sizes. RESULTS: Blood cells greater than 12 μm were guided to a special branch, which improved separation efficiency because it prevented the deposition of particles in other branches. The designed device had the capability to separate blood cells with diameters of 2.0 μm, 6.2 μm, 10.0 μm, and greater than 12.0 μm. The applied voltage to the electrodes was 50 V with a frequency of 100 kHz. CONCLUSION: The proposed device is a simple, efficient DEP-based continuous cell separator. This capability makes it ideal for use in various biomedical applications, including cell therapy and cell separation, and results in a throughput increment of microfluidics devices. Royan Institute 2020 2019-10-14 /pmc/articles/PMC6874797/ /pubmed/31721537 http://dx.doi.org/10.22074/cellj.2020.6386 Text en The Cell Journal (Yakhteh) is an open access journal which means the articles are freely available online for any individual author to download and use the providing address. The journal is licensed under a Creative Commons Attribution-Non Commercial 3.0 Unported License which allows the author(s) to hold the copyright without restrictions that is permitting unrestricted use, distribution, and reproduction in any medium provided the original work is properly cited. http://creativecommons.org/licenses/by/3/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Zahedi Siani, Omid Sojoodi, Mahdi Zabetian Targhi, Mohammad Movahedin, Mansoureh Blood Particle Separation Using Dielectrophoresis in A Novel Microchannel: A Numerical Study |
title | Blood Particle Separation Using Dielectrophoresis in A Novel
Microchannel: A Numerical Study |
title_full | Blood Particle Separation Using Dielectrophoresis in A Novel
Microchannel: A Numerical Study |
title_fullStr | Blood Particle Separation Using Dielectrophoresis in A Novel
Microchannel: A Numerical Study |
title_full_unstemmed | Blood Particle Separation Using Dielectrophoresis in A Novel
Microchannel: A Numerical Study |
title_short | Blood Particle Separation Using Dielectrophoresis in A Novel
Microchannel: A Numerical Study |
title_sort | blood particle separation using dielectrophoresis in a novel
microchannel: a numerical study |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6874797/ https://www.ncbi.nlm.nih.gov/pubmed/31721537 http://dx.doi.org/10.22074/cellj.2020.6386 |
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