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Complementary Embryonic and Adult Cell Populations Enhance Myocardial Repair in Rat Myocardial Injury Model
We compared the functional outcome of Isl-1(+) cardiac progenitors, CD90(+) bone marrow-derived progenitor cells, and the combination of the two in a rat myocardial infarction (MI) model. Isl-1(+) cells were isolated from embryonic day 12.5 (E12.5) rat hearts and expanded in vitro. Thy-1(+)/CD90(+)...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6875168/ https://www.ncbi.nlm.nih.gov/pubmed/31781239 http://dx.doi.org/10.1155/2019/3945850 |
Sumario: | We compared the functional outcome of Isl-1(+) cardiac progenitors, CD90(+) bone marrow-derived progenitor cells, and the combination of the two in a rat myocardial infarction (MI) model. Isl-1(+) cells were isolated from embryonic day 12.5 (E12.5) rat hearts and expanded in vitro. Thy-1(+)/CD90(+) cells were isolated from the bone marrow of adult Sprague-Dawley rats by immunomagnetic cell sorting. Six-week-old female Sprague-Dawley rats underwent permanent left anterior descending (LAD) coronary artery ligation and received intramyocardial injection of either saline, Isl-1(+) cells, CD90(+) cells, or a combination of Isl-1(+) and CD90(+) cells, at the time of infarction. Cells were delivered transepicardially to the peri-infarct zone. Left ventricular function was assessed by transthoracic echocardiography at 1- and 4-week post-MI and by Millar catheterization (-dP/dt and +dP/dt) at 4-week post-MI. Fluorescence in situ hybridization (Isl-1(+)cells) and monochrystalline iron oxide nanoparticles labeling (MION; CD90(+) cells) were performed to assess biodistribution of transplanted cells. Only the combination of cells demonstrated a significant improvement of cardiac function as assessed by anterior wall contractility, dP/dt (max), and dP/dt (min), compared to Isl-1(+) or CD90(+) cell monotherapies. In the combination cell group, viable cells were detected at week 4 when anterior wall motion was completely restored. In conclusion, the combination of Isl-1(+) cardiac progenitors and adult bone marrow-derived CD90(+) cells shows prolonged and robust myocardial tissue repair and provides support for the use of complementary cell populations to enhance myocardial repair. |
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