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Effect of Zinc Supplementation on Urate Pathway Enzymes in Spermatozoa and Seminal Plasma of Iraqi Asthenozoospermic Patients: A Randomized Controlled Trial
BACKGROUND: Uric acid (UA) is crucial for sperm metabolism as it protects seminal plasma against oxidative dam- age. Zinc also plays a central role in sperm metabolism. The current study was designed to investigate the role of zinc supplementation on qualitative and quantitative properties of semina...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Royan Institute
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6875853/ https://www.ncbi.nlm.nih.gov/pubmed/31710193 http://dx.doi.org/10.22074/ijfs.2020.5760 |
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author | Alsalman, Abdul Razzaq S. Almashhedy, Lamia A. Alta'ee, Abdulsamie H. Hadwan, Mahmoud H. |
author_facet | Alsalman, Abdul Razzaq S. Almashhedy, Lamia A. Alta'ee, Abdulsamie H. Hadwan, Mahmoud H. |
author_sort | Alsalman, Abdul Razzaq S. |
collection | PubMed |
description | BACKGROUND: Uric acid (UA) is crucial for sperm metabolism as it protects seminal plasma against oxidative dam- age. Zinc also plays a central role in sperm metabolism. The current study was designed to investigate the role of zinc supplementation on qualitative and quantitative properties of seminal fluid, in parallel with the UA level and urate pathway enzymes in the semen of patients with asthenozoospermia. MATERIALS AND METHODS: The study was designed as a randomized controlled trial of 60 asthenozoospermic subfertile men. The current study, which was conducted during one year, involved 60 fertile and 60 asthenozoospermic subfertile men belonging to Hilla City, Iraq. Semen samples were obtained from the participants before and after treatment with zinc supplements. The levels of UA, xanthine oxidase (XO), adenosine deaminase (ADA) and 5'-nucleotidase (5'-NU) activities were determined in spermatozoa and seminal plasma of both groups. RESULTS: UA levels (P=0.034) and 5'-NU activity (P=0.046) were significantly lower but ADA (P=0.05) and XO (P=0.015) activities were significantly higher in infertile men than in healthy men. Treatment with zinc sulfate induced an increase in UA (P=0.001) level and 5'-NU activity (P=0.001), but a decrease in ADA (P=0.016) and XO (P=0.05) activities. CONCLUSION: Zinc supplementation restores UA levels and the activities of enzymes involved in the urate pathway (XO and ADA) in the seminal plasma and spermatozoa of patients with asthenozoospermia, to reference values. Sup- plementation of Zn compounds enhances the qualitative and quantitative properties of semen (Registration number: NCT03361618). |
format | Online Article Text |
id | pubmed-6875853 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Royan Institute |
record_format | MEDLINE/PubMed |
spelling | pubmed-68758532020-01-01 Effect of Zinc Supplementation on Urate Pathway Enzymes in Spermatozoa and Seminal Plasma of Iraqi Asthenozoospermic Patients: A Randomized Controlled Trial Alsalman, Abdul Razzaq S. Almashhedy, Lamia A. Alta'ee, Abdulsamie H. Hadwan, Mahmoud H. Int J Fertil Steril Original Article BACKGROUND: Uric acid (UA) is crucial for sperm metabolism as it protects seminal plasma against oxidative dam- age. Zinc also plays a central role in sperm metabolism. The current study was designed to investigate the role of zinc supplementation on qualitative and quantitative properties of seminal fluid, in parallel with the UA level and urate pathway enzymes in the semen of patients with asthenozoospermia. MATERIALS AND METHODS: The study was designed as a randomized controlled trial of 60 asthenozoospermic subfertile men. The current study, which was conducted during one year, involved 60 fertile and 60 asthenozoospermic subfertile men belonging to Hilla City, Iraq. Semen samples were obtained from the participants before and after treatment with zinc supplements. The levels of UA, xanthine oxidase (XO), adenosine deaminase (ADA) and 5'-nucleotidase (5'-NU) activities were determined in spermatozoa and seminal plasma of both groups. RESULTS: UA levels (P=0.034) and 5'-NU activity (P=0.046) were significantly lower but ADA (P=0.05) and XO (P=0.015) activities were significantly higher in infertile men than in healthy men. Treatment with zinc sulfate induced an increase in UA (P=0.001) level and 5'-NU activity (P=0.001), but a decrease in ADA (P=0.016) and XO (P=0.05) activities. CONCLUSION: Zinc supplementation restores UA levels and the activities of enzymes involved in the urate pathway (XO and ADA) in the seminal plasma and spermatozoa of patients with asthenozoospermia, to reference values. Sup- plementation of Zn compounds enhances the qualitative and quantitative properties of semen (Registration number: NCT03361618). Royan Institute 2020 2019-11-11 /pmc/articles/PMC6875853/ /pubmed/31710193 http://dx.doi.org/10.22074/ijfs.2020.5760 Text en The Cell Journal (Yakhteh) is an open access journal which means the articles are freely available online for any individual author to download and use the providing address. The journal is licensed under a Creative Commons Attribution-Non Commercial 3.0 Unported License which allows the author(s) to hold the copyright without restrictions that is permitting unrestricted use, distribution, and reproduction in any medium provided the original work is properly cited. http://creativecommons.org/licenses/by/3/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Alsalman, Abdul Razzaq S. Almashhedy, Lamia A. Alta'ee, Abdulsamie H. Hadwan, Mahmoud H. Effect of Zinc Supplementation on Urate Pathway Enzymes in Spermatozoa and Seminal Plasma of Iraqi Asthenozoospermic Patients: A Randomized Controlled Trial |
title | Effect of Zinc Supplementation on Urate Pathway Enzymes in
Spermatozoa and Seminal Plasma of Iraqi Asthenozoospermic
Patients: A Randomized Controlled Trial |
title_full | Effect of Zinc Supplementation on Urate Pathway Enzymes in
Spermatozoa and Seminal Plasma of Iraqi Asthenozoospermic
Patients: A Randomized Controlled Trial |
title_fullStr | Effect of Zinc Supplementation on Urate Pathway Enzymes in
Spermatozoa and Seminal Plasma of Iraqi Asthenozoospermic
Patients: A Randomized Controlled Trial |
title_full_unstemmed | Effect of Zinc Supplementation on Urate Pathway Enzymes in
Spermatozoa and Seminal Plasma of Iraqi Asthenozoospermic
Patients: A Randomized Controlled Trial |
title_short | Effect of Zinc Supplementation on Urate Pathway Enzymes in
Spermatozoa and Seminal Plasma of Iraqi Asthenozoospermic
Patients: A Randomized Controlled Trial |
title_sort | effect of zinc supplementation on urate pathway enzymes in
spermatozoa and seminal plasma of iraqi asthenozoospermic
patients: a randomized controlled trial |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6875853/ https://www.ncbi.nlm.nih.gov/pubmed/31710193 http://dx.doi.org/10.22074/ijfs.2020.5760 |
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