Cargando…

Differentiation and roles of bone marrow-derived cells on the tumor microenvironment of oral squamous cell carcinoma

The stroma affects the properties and dynamics of the tumor. Previous studies have demonstrated that bone marrow-derived cells (BMDCs) possess the capability of differentiating into stromal cells. However, the characteristics and roles of BMDCs in oral squamous cell carcinoma remain unclear. The cur...

Descripción completa

Detalles Bibliográficos
Autores principales: Anqi, Chang, Takabatake, Kiyofumi, Kawai, Hotaka, Oo, May Wathone, Yoshida, Saori, Fujii, Masae, Omori, Haruka, Sukegawa, Shintaro, Nakano, Keisuke, Tsujigiwa, Hidetsugu, Jinhua, Zheng, Nagatsuka, Hitoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6876317/
https://www.ncbi.nlm.nih.gov/pubmed/31807176
http://dx.doi.org/10.3892/ol.2019.11045
_version_ 1783473187380527104
author Anqi, Chang
Takabatake, Kiyofumi
Kawai, Hotaka
Oo, May Wathone
Yoshida, Saori
Fujii, Masae
Omori, Haruka
Sukegawa, Shintaro
Nakano, Keisuke
Tsujigiwa, Hidetsugu
Jinhua, Zheng
Nagatsuka, Hitoshi
author_facet Anqi, Chang
Takabatake, Kiyofumi
Kawai, Hotaka
Oo, May Wathone
Yoshida, Saori
Fujii, Masae
Omori, Haruka
Sukegawa, Shintaro
Nakano, Keisuke
Tsujigiwa, Hidetsugu
Jinhua, Zheng
Nagatsuka, Hitoshi
author_sort Anqi, Chang
collection PubMed
description The stroma affects the properties and dynamics of the tumor. Previous studies have demonstrated that bone marrow-derived cells (BMDCs) possess the capability of differentiating into stromal cells. However, the characteristics and roles of BMDCs in oral squamous cell carcinoma remain unclear. The current study therefore investigated their locations and features by tracing green fluorescent protein (GFP)-labeled BMDCs in a transplantation mouse model. After irradiation, BALB-c nu-nu mice were injected with bone marrow cells from C57BL/6-BALB-C-nu/nu-GFP transgenic mice. These recipient mice were then injected subcutaneously in the head with human squamous cell carcinoma-2 cells. Immunohistochemistry for GFP, Vimentin, CD11b, CD31 and α-smooth muscle actin (SMA), and double-fluorescent immunohistochemistry for GFP-Vimentin, GFP-CD11b, GFP-CD31 and GFP-α-SMA was subsequently performed. Many round-shaped GFP-positive cells were observed in the cancer stroma, which indicated that BMDCs served a predominant role in tumorigenesis. Vimentin(+) GFP(+) cells may also be a member of the cancer-associated stroma, originating from bone marrow. Round or spindle-shaped CD11b(+) GFP(+) cells identified in the present study may be macrophages derived from bone marrow. CD31(+)GFP(+) cells exhibited a high tendency towards bone marrow-derived angioblasts. The results also indicated that spindle-shaped α-SMA(+) GFP(+) cells were not likely to represent bone marrow-derived cancer-associated fibroblasts. BMDCs gathering within the tumor microenvironment exhibited multilineage potency and participated in several important processes, such as tumorigenesis, tumor invasion and angiogenesis.
format Online
Article
Text
id pubmed-6876317
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher D.A. Spandidos
record_format MEDLINE/PubMed
spelling pubmed-68763172019-12-05 Differentiation and roles of bone marrow-derived cells on the tumor microenvironment of oral squamous cell carcinoma Anqi, Chang Takabatake, Kiyofumi Kawai, Hotaka Oo, May Wathone Yoshida, Saori Fujii, Masae Omori, Haruka Sukegawa, Shintaro Nakano, Keisuke Tsujigiwa, Hidetsugu Jinhua, Zheng Nagatsuka, Hitoshi Oncol Lett Articles The stroma affects the properties and dynamics of the tumor. Previous studies have demonstrated that bone marrow-derived cells (BMDCs) possess the capability of differentiating into stromal cells. However, the characteristics and roles of BMDCs in oral squamous cell carcinoma remain unclear. The current study therefore investigated their locations and features by tracing green fluorescent protein (GFP)-labeled BMDCs in a transplantation mouse model. After irradiation, BALB-c nu-nu mice were injected with bone marrow cells from C57BL/6-BALB-C-nu/nu-GFP transgenic mice. These recipient mice were then injected subcutaneously in the head with human squamous cell carcinoma-2 cells. Immunohistochemistry for GFP, Vimentin, CD11b, CD31 and α-smooth muscle actin (SMA), and double-fluorescent immunohistochemistry for GFP-Vimentin, GFP-CD11b, GFP-CD31 and GFP-α-SMA was subsequently performed. Many round-shaped GFP-positive cells were observed in the cancer stroma, which indicated that BMDCs served a predominant role in tumorigenesis. Vimentin(+) GFP(+) cells may also be a member of the cancer-associated stroma, originating from bone marrow. Round or spindle-shaped CD11b(+) GFP(+) cells identified in the present study may be macrophages derived from bone marrow. CD31(+)GFP(+) cells exhibited a high tendency towards bone marrow-derived angioblasts. The results also indicated that spindle-shaped α-SMA(+) GFP(+) cells were not likely to represent bone marrow-derived cancer-associated fibroblasts. BMDCs gathering within the tumor microenvironment exhibited multilineage potency and participated in several important processes, such as tumorigenesis, tumor invasion and angiogenesis. D.A. Spandidos 2019-12 2019-11-04 /pmc/articles/PMC6876317/ /pubmed/31807176 http://dx.doi.org/10.3892/ol.2019.11045 Text en Copyright: © Anqi et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Anqi, Chang
Takabatake, Kiyofumi
Kawai, Hotaka
Oo, May Wathone
Yoshida, Saori
Fujii, Masae
Omori, Haruka
Sukegawa, Shintaro
Nakano, Keisuke
Tsujigiwa, Hidetsugu
Jinhua, Zheng
Nagatsuka, Hitoshi
Differentiation and roles of bone marrow-derived cells on the tumor microenvironment of oral squamous cell carcinoma
title Differentiation and roles of bone marrow-derived cells on the tumor microenvironment of oral squamous cell carcinoma
title_full Differentiation and roles of bone marrow-derived cells on the tumor microenvironment of oral squamous cell carcinoma
title_fullStr Differentiation and roles of bone marrow-derived cells on the tumor microenvironment of oral squamous cell carcinoma
title_full_unstemmed Differentiation and roles of bone marrow-derived cells on the tumor microenvironment of oral squamous cell carcinoma
title_short Differentiation and roles of bone marrow-derived cells on the tumor microenvironment of oral squamous cell carcinoma
title_sort differentiation and roles of bone marrow-derived cells on the tumor microenvironment of oral squamous cell carcinoma
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6876317/
https://www.ncbi.nlm.nih.gov/pubmed/31807176
http://dx.doi.org/10.3892/ol.2019.11045
work_keys_str_mv AT anqichang differentiationandrolesofbonemarrowderivedcellsonthetumormicroenvironmentoforalsquamouscellcarcinoma
AT takabatakekiyofumi differentiationandrolesofbonemarrowderivedcellsonthetumormicroenvironmentoforalsquamouscellcarcinoma
AT kawaihotaka differentiationandrolesofbonemarrowderivedcellsonthetumormicroenvironmentoforalsquamouscellcarcinoma
AT oomaywathone differentiationandrolesofbonemarrowderivedcellsonthetumormicroenvironmentoforalsquamouscellcarcinoma
AT yoshidasaori differentiationandrolesofbonemarrowderivedcellsonthetumormicroenvironmentoforalsquamouscellcarcinoma
AT fujiimasae differentiationandrolesofbonemarrowderivedcellsonthetumormicroenvironmentoforalsquamouscellcarcinoma
AT omoriharuka differentiationandrolesofbonemarrowderivedcellsonthetumormicroenvironmentoforalsquamouscellcarcinoma
AT sukegawashintaro differentiationandrolesofbonemarrowderivedcellsonthetumormicroenvironmentoforalsquamouscellcarcinoma
AT nakanokeisuke differentiationandrolesofbonemarrowderivedcellsonthetumormicroenvironmentoforalsquamouscellcarcinoma
AT tsujigiwahidetsugu differentiationandrolesofbonemarrowderivedcellsonthetumormicroenvironmentoforalsquamouscellcarcinoma
AT jinhuazheng differentiationandrolesofbonemarrowderivedcellsonthetumormicroenvironmentoforalsquamouscellcarcinoma
AT nagatsukahitoshi differentiationandrolesofbonemarrowderivedcellsonthetumormicroenvironmentoforalsquamouscellcarcinoma