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Gastric mucosal devitalization improves blood pressure, renin and cardiovascular lipid deposition in a rat model of obesity
Background and study aims In lieu of the drawbacks of metabolic surgery, a method of mimicking resection of the gastric mucosa could be of value to those with obesity-related cardiovascular disease (CVD). Our study aims to investigate the effect of gastric mucosal devitalization (GMD) on blood pres...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
© Georg Thieme Verlag KG
2019
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6877424/ https://www.ncbi.nlm.nih.gov/pubmed/31788541 http://dx.doi.org/10.1055/a-0990-9683 |
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author | Oberbach, Andreas Schlichting, Nadine Kullnick, Yvonne Heinrich, Marco Lehmann, Stefanie Retschlag, Ulf Friedrich, Maik Fayad, Lea Dietrich, Arne Khashab, Mouen A. Kalloo, Anthony N. Kumbhari, Vivek |
author_facet | Oberbach, Andreas Schlichting, Nadine Kullnick, Yvonne Heinrich, Marco Lehmann, Stefanie Retschlag, Ulf Friedrich, Maik Fayad, Lea Dietrich, Arne Khashab, Mouen A. Kalloo, Anthony N. Kumbhari, Vivek |
author_sort | Oberbach, Andreas |
collection | PubMed |
description | Background and study aims In lieu of the drawbacks of metabolic surgery, a method of mimicking resection of the gastric mucosa could be of value to those with obesity-related cardiovascular disease (CVD). Our study aims to investigate the effect of gastric mucosal devitalization (GMD) on blood pressure (BP) and cardiovascular lipid deposition in a rat model of obesity. Methods GMD of 70 % of the stomach was achieved by argon plasma coagulation. GMD was compared to sleeve gastrectomy (SG) and sham (SH) in a high-fat-diet-induced rat model of obesity (48 rats). At 8 weeks, we measured noninvasive BP, renin, vessel relaxation and ghrelin receptor regulation in the aorta. In addition, we quantified cardiac lipid deposition and lipid droplet deposition in cardiac muscle and aorta. Results GMD and SG were observed to have similar reductions in body weight, visceral adiposity, and serum lipid profile compared to SH rats. GMD resulted in a significant reduction in arterial BP compared to SH. Furthermore, there were significant reductions in plasma renin activity and percentage of phenylnephrine constriction to acetylcholine at the aortic ring in GMD rats compared to SH, providing insights into the mechanisms behind the reduced BP. Interestingly, the reduced BP occurred despite a reduction in endothelial ghrelin recteptor activation. Cardiac lipid content was significantly reduced in GMD rats. Lipid deposition, as illustrated by Nile Red stain, was reduced in cardiac muscle and the aorta. Conclusion GMD resulted in a significant improvement in BP, renin and cardiovascular lipid deposition. GMD deserves further attention as a method of treating obesity-related CVD. |
format | Online Article Text |
id | pubmed-6877424 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | © Georg Thieme Verlag KG |
record_format | MEDLINE/PubMed |
spelling | pubmed-68774242019-12-01 Gastric mucosal devitalization improves blood pressure, renin and cardiovascular lipid deposition in a rat model of obesity Oberbach, Andreas Schlichting, Nadine Kullnick, Yvonne Heinrich, Marco Lehmann, Stefanie Retschlag, Ulf Friedrich, Maik Fayad, Lea Dietrich, Arne Khashab, Mouen A. Kalloo, Anthony N. Kumbhari, Vivek Endosc Int Open Background and study aims In lieu of the drawbacks of metabolic surgery, a method of mimicking resection of the gastric mucosa could be of value to those with obesity-related cardiovascular disease (CVD). Our study aims to investigate the effect of gastric mucosal devitalization (GMD) on blood pressure (BP) and cardiovascular lipid deposition in a rat model of obesity. Methods GMD of 70 % of the stomach was achieved by argon plasma coagulation. GMD was compared to sleeve gastrectomy (SG) and sham (SH) in a high-fat-diet-induced rat model of obesity (48 rats). At 8 weeks, we measured noninvasive BP, renin, vessel relaxation and ghrelin receptor regulation in the aorta. In addition, we quantified cardiac lipid deposition and lipid droplet deposition in cardiac muscle and aorta. Results GMD and SG were observed to have similar reductions in body weight, visceral adiposity, and serum lipid profile compared to SH rats. GMD resulted in a significant reduction in arterial BP compared to SH. Furthermore, there were significant reductions in plasma renin activity and percentage of phenylnephrine constriction to acetylcholine at the aortic ring in GMD rats compared to SH, providing insights into the mechanisms behind the reduced BP. Interestingly, the reduced BP occurred despite a reduction in endothelial ghrelin recteptor activation. Cardiac lipid content was significantly reduced in GMD rats. Lipid deposition, as illustrated by Nile Red stain, was reduced in cardiac muscle and the aorta. Conclusion GMD resulted in a significant improvement in BP, renin and cardiovascular lipid deposition. GMD deserves further attention as a method of treating obesity-related CVD. © Georg Thieme Verlag KG 2019-12 2019-11-25 /pmc/articles/PMC6877424/ /pubmed/31788541 http://dx.doi.org/10.1055/a-0990-9683 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License, which permits unrestricted reproduction and distribution, for non-commercial purposes only; and use and reproduction, but not distribution, of adapted material for non-commercial purposes only, provided the original work is properly cited. |
spellingShingle | Oberbach, Andreas Schlichting, Nadine Kullnick, Yvonne Heinrich, Marco Lehmann, Stefanie Retschlag, Ulf Friedrich, Maik Fayad, Lea Dietrich, Arne Khashab, Mouen A. Kalloo, Anthony N. Kumbhari, Vivek Gastric mucosal devitalization improves blood pressure, renin and cardiovascular lipid deposition in a rat model of obesity |
title | Gastric mucosal devitalization improves blood pressure, renin and cardiovascular lipid deposition in a rat model of obesity |
title_full | Gastric mucosal devitalization improves blood pressure, renin and cardiovascular lipid deposition in a rat model of obesity |
title_fullStr | Gastric mucosal devitalization improves blood pressure, renin and cardiovascular lipid deposition in a rat model of obesity |
title_full_unstemmed | Gastric mucosal devitalization improves blood pressure, renin and cardiovascular lipid deposition in a rat model of obesity |
title_short | Gastric mucosal devitalization improves blood pressure, renin and cardiovascular lipid deposition in a rat model of obesity |
title_sort | gastric mucosal devitalization improves blood pressure, renin and cardiovascular lipid deposition in a rat model of obesity |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6877424/ https://www.ncbi.nlm.nih.gov/pubmed/31788541 http://dx.doi.org/10.1055/a-0990-9683 |
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