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JASPer controls interphase histone H3S10 phosphorylation by chromosomal kinase JIL-1 in Drosophila

In flies, the chromosomal kinase JIL-1 is responsible for most interphase histone H3S10 phosphorylation and has been proposed to protect active chromatin from acquiring heterochromatic marks, such as dimethylated histone H3K9 (H3K9me2) and HP1. Here, we show that JIL-1’s targeting to chromatin depen...

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Autores principales: Albig, Christian, Wang, Chao, Dann, Geoffrey P., Wojcik, Felix, Schauer, Tamás, Krause, Silke, Maenner, Sylvain, Cai, Weili, Li, Yeran, Girton, Jack, Muir, Tom W., Johansen, Jørgen, Johansen, Kristen M., Becker, Peter B., Regnard, Catherine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6877644/
https://www.ncbi.nlm.nih.gov/pubmed/31767855
http://dx.doi.org/10.1038/s41467-019-13174-6
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author Albig, Christian
Wang, Chao
Dann, Geoffrey P.
Wojcik, Felix
Schauer, Tamás
Krause, Silke
Maenner, Sylvain
Cai, Weili
Li, Yeran
Girton, Jack
Muir, Tom W.
Johansen, Jørgen
Johansen, Kristen M.
Becker, Peter B.
Regnard, Catherine
author_facet Albig, Christian
Wang, Chao
Dann, Geoffrey P.
Wojcik, Felix
Schauer, Tamás
Krause, Silke
Maenner, Sylvain
Cai, Weili
Li, Yeran
Girton, Jack
Muir, Tom W.
Johansen, Jørgen
Johansen, Kristen M.
Becker, Peter B.
Regnard, Catherine
author_sort Albig, Christian
collection PubMed
description In flies, the chromosomal kinase JIL-1 is responsible for most interphase histone H3S10 phosphorylation and has been proposed to protect active chromatin from acquiring heterochromatic marks, such as dimethylated histone H3K9 (H3K9me2) and HP1. Here, we show that JIL-1’s targeting to chromatin depends on a PWWP domain-containing protein JASPer (JIL-1 Anchoring and Stabilizing Protein). JASPer-JIL-1 (JJ)-complex is the major form of kinase in vivo and is targeted to active genes and telomeric transposons via binding of the PWWP domain of JASPer to H3K36me3 nucleosomes, to modulate transcriptional output. JIL-1 and JJ-complex depletion in cycling cells lead to small changes in H3K9me2 distribution at active genes and telomeric transposons. Finally, we identify interactors of the endogenous JJ-complex and propose that JIL-1 not only prevents heterochromatin formation but also coordinates chromatin-based regulation in the transcribed part of the genome.
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spelling pubmed-68776442019-11-27 JASPer controls interphase histone H3S10 phosphorylation by chromosomal kinase JIL-1 in Drosophila Albig, Christian Wang, Chao Dann, Geoffrey P. Wojcik, Felix Schauer, Tamás Krause, Silke Maenner, Sylvain Cai, Weili Li, Yeran Girton, Jack Muir, Tom W. Johansen, Jørgen Johansen, Kristen M. Becker, Peter B. Regnard, Catherine Nat Commun Article In flies, the chromosomal kinase JIL-1 is responsible for most interphase histone H3S10 phosphorylation and has been proposed to protect active chromatin from acquiring heterochromatic marks, such as dimethylated histone H3K9 (H3K9me2) and HP1. Here, we show that JIL-1’s targeting to chromatin depends on a PWWP domain-containing protein JASPer (JIL-1 Anchoring and Stabilizing Protein). JASPer-JIL-1 (JJ)-complex is the major form of kinase in vivo and is targeted to active genes and telomeric transposons via binding of the PWWP domain of JASPer to H3K36me3 nucleosomes, to modulate transcriptional output. JIL-1 and JJ-complex depletion in cycling cells lead to small changes in H3K9me2 distribution at active genes and telomeric transposons. Finally, we identify interactors of the endogenous JJ-complex and propose that JIL-1 not only prevents heterochromatin formation but also coordinates chromatin-based regulation in the transcribed part of the genome. Nature Publishing Group UK 2019-11-25 /pmc/articles/PMC6877644/ /pubmed/31767855 http://dx.doi.org/10.1038/s41467-019-13174-6 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Albig, Christian
Wang, Chao
Dann, Geoffrey P.
Wojcik, Felix
Schauer, Tamás
Krause, Silke
Maenner, Sylvain
Cai, Weili
Li, Yeran
Girton, Jack
Muir, Tom W.
Johansen, Jørgen
Johansen, Kristen M.
Becker, Peter B.
Regnard, Catherine
JASPer controls interphase histone H3S10 phosphorylation by chromosomal kinase JIL-1 in Drosophila
title JASPer controls interphase histone H3S10 phosphorylation by chromosomal kinase JIL-1 in Drosophila
title_full JASPer controls interphase histone H3S10 phosphorylation by chromosomal kinase JIL-1 in Drosophila
title_fullStr JASPer controls interphase histone H3S10 phosphorylation by chromosomal kinase JIL-1 in Drosophila
title_full_unstemmed JASPer controls interphase histone H3S10 phosphorylation by chromosomal kinase JIL-1 in Drosophila
title_short JASPer controls interphase histone H3S10 phosphorylation by chromosomal kinase JIL-1 in Drosophila
title_sort jasper controls interphase histone h3s10 phosphorylation by chromosomal kinase jil-1 in drosophila
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6877644/
https://www.ncbi.nlm.nih.gov/pubmed/31767855
http://dx.doi.org/10.1038/s41467-019-13174-6
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