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Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H(2)O(2)-Induced DNA Damage in Human Whole Blood Cells
The health benefits of natural products have long been recognized. Consumption of dietary compounds such as supplements provides an alternative source of natural products to those obtained from the diet. There is a growing concern regarding the possible side effects of using different food supplemen...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6878777/ https://www.ncbi.nlm.nih.gov/pubmed/31814882 http://dx.doi.org/10.1155/2019/5039372 |
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author | Živković, Lada Bajić, Vladan Topalović, Dijana Bruić, Marija Spremo-Potparević, Biljana |
author_facet | Živković, Lada Bajić, Vladan Topalović, Dijana Bruić, Marija Spremo-Potparević, Biljana |
author_sort | Živković, Lada |
collection | PubMed |
description | The health benefits of natural products have long been recognized. Consumption of dietary compounds such as supplements provides an alternative source of natural products to those obtained from the diet. There is a growing concern regarding the possible side effects of using different food supplements simultaneously, since their possible interactions are less known. For the first time, we have tested genotoxic and antigenotoxic effects of Biochaga, in combination with dihydroquercetin. No genotoxic effect on whole blood cells was observed within individual treatment of Biochaga (250 μg/mL, 500 μg/mL and 1000 μg/mL) and dihydroquercetin (100 μg/mL, 250 μg/mL and 500 μg/mL), nor in combination. Afterwards, antigenotoxic potency of both supplements against hydrogen peroxide- (H(2)O(2)-) induced DNA damage to whole blood cells (WBC) was assessed, using the comet assay. Biochaga and dihydroquercetin displayed a strong potential to attenuate H(2)O(2)-induced damage on DNA in cells at all tested concentrations, with a statistical significance (p < 0.05), whereas Biochaga at the dose of 500 μg/mL in combination with dihydroquercetin 500 μg/mL was most prominent. Biochaga in combination with dihydroquercetin is able to protect genomic material from oxidative damage induced by hydrogen peroxide in vitro. |
format | Online Article Text |
id | pubmed-6878777 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-68787772019-12-08 Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H(2)O(2)-Induced DNA Damage in Human Whole Blood Cells Živković, Lada Bajić, Vladan Topalović, Dijana Bruić, Marija Spremo-Potparević, Biljana Oxid Med Cell Longev Research Article The health benefits of natural products have long been recognized. Consumption of dietary compounds such as supplements provides an alternative source of natural products to those obtained from the diet. There is a growing concern regarding the possible side effects of using different food supplements simultaneously, since their possible interactions are less known. For the first time, we have tested genotoxic and antigenotoxic effects of Biochaga, in combination with dihydroquercetin. No genotoxic effect on whole blood cells was observed within individual treatment of Biochaga (250 μg/mL, 500 μg/mL and 1000 μg/mL) and dihydroquercetin (100 μg/mL, 250 μg/mL and 500 μg/mL), nor in combination. Afterwards, antigenotoxic potency of both supplements against hydrogen peroxide- (H(2)O(2)-) induced DNA damage to whole blood cells (WBC) was assessed, using the comet assay. Biochaga and dihydroquercetin displayed a strong potential to attenuate H(2)O(2)-induced damage on DNA in cells at all tested concentrations, with a statistical significance (p < 0.05), whereas Biochaga at the dose of 500 μg/mL in combination with dihydroquercetin 500 μg/mL was most prominent. Biochaga in combination with dihydroquercetin is able to protect genomic material from oxidative damage induced by hydrogen peroxide in vitro. Hindawi 2019-11-11 /pmc/articles/PMC6878777/ /pubmed/31814882 http://dx.doi.org/10.1155/2019/5039372 Text en Copyright © 2019 Lada Živković et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Živković, Lada Bajić, Vladan Topalović, Dijana Bruić, Marija Spremo-Potparević, Biljana Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H(2)O(2)-Induced DNA Damage in Human Whole Blood Cells |
title | Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H(2)O(2)-Induced DNA Damage in Human Whole Blood Cells |
title_full | Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H(2)O(2)-Induced DNA Damage in Human Whole Blood Cells |
title_fullStr | Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H(2)O(2)-Induced DNA Damage in Human Whole Blood Cells |
title_full_unstemmed | Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H(2)O(2)-Induced DNA Damage in Human Whole Blood Cells |
title_short | Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H(2)O(2)-Induced DNA Damage in Human Whole Blood Cells |
title_sort | antigenotoxic effects of biochaga and dihydroquercetin (taxifolin) on h(2)o(2)-induced dna damage in human whole blood cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6878777/ https://www.ncbi.nlm.nih.gov/pubmed/31814882 http://dx.doi.org/10.1155/2019/5039372 |
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