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Dissecting the Roles of GABA and Neuropeptides from Rat Central Amygdala CRF Neurons in Anxiety and Fear Learning

Central amygdala (CeA) neurons that produce corticotropin-releasing factor (CRF) regulate anxiety and fear learning. These CeA(CRF) neurons release GABA and several neuropeptides predicted to play important yet opposing roles in these behaviors. We dissected the relative roles of GABA, CRF, dynorphi...

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Autores principales: Pomrenze, Matthew B., Giovanetti, Simone M., Maiya, Rajani, Gordon, Adam G., Kreeger, Lauren J., Messing, Robert O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6879108/
https://www.ncbi.nlm.nih.gov/pubmed/31577943
http://dx.doi.org/10.1016/j.celrep.2019.08.083
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author Pomrenze, Matthew B.
Giovanetti, Simone M.
Maiya, Rajani
Gordon, Adam G.
Kreeger, Lauren J.
Messing, Robert O.
author_facet Pomrenze, Matthew B.
Giovanetti, Simone M.
Maiya, Rajani
Gordon, Adam G.
Kreeger, Lauren J.
Messing, Robert O.
author_sort Pomrenze, Matthew B.
collection PubMed
description Central amygdala (CeA) neurons that produce corticotropin-releasing factor (CRF) regulate anxiety and fear learning. These CeA(CRF) neurons release GABA and several neuropeptides predicted to play important yet opposing roles in these behaviors. We dissected the relative roles of GABA, CRF, dynorphin, and neurotensin in CeA(CRF) neurons in anxiety and fear learning by disrupting their expression using RNAi in male rats. GABA, but not CRF, dynorphin, or neurotensin, regulates baseline anxiety-like behavior. In contrast, chemogenetic stimulation of CeA(CRF) neurons evokes anxiety-like behavior dependent on CRF and dynorphin, but not neurotensin. Finally, knockdown of CRF and dynorphin impairs fear learning, whereas knockdown of neurotensin enhances it. Our results demonstrate distinct behavioral roles for GABA, CRF, dynorphin, and neurotensin in a subpopulation of CeA neurons. These results highlight the importance of considering the repertoire of signaling molecules released from a given neuronal population when studying the circuit basis of behavior.
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spelling pubmed-68791082019-11-26 Dissecting the Roles of GABA and Neuropeptides from Rat Central Amygdala CRF Neurons in Anxiety and Fear Learning Pomrenze, Matthew B. Giovanetti, Simone M. Maiya, Rajani Gordon, Adam G. Kreeger, Lauren J. Messing, Robert O. Cell Rep Article Central amygdala (CeA) neurons that produce corticotropin-releasing factor (CRF) regulate anxiety and fear learning. These CeA(CRF) neurons release GABA and several neuropeptides predicted to play important yet opposing roles in these behaviors. We dissected the relative roles of GABA, CRF, dynorphin, and neurotensin in CeA(CRF) neurons in anxiety and fear learning by disrupting their expression using RNAi in male rats. GABA, but not CRF, dynorphin, or neurotensin, regulates baseline anxiety-like behavior. In contrast, chemogenetic stimulation of CeA(CRF) neurons evokes anxiety-like behavior dependent on CRF and dynorphin, but not neurotensin. Finally, knockdown of CRF and dynorphin impairs fear learning, whereas knockdown of neurotensin enhances it. Our results demonstrate distinct behavioral roles for GABA, CRF, dynorphin, and neurotensin in a subpopulation of CeA neurons. These results highlight the importance of considering the repertoire of signaling molecules released from a given neuronal population when studying the circuit basis of behavior. 2019-10-01 /pmc/articles/PMC6879108/ /pubmed/31577943 http://dx.doi.org/10.1016/j.celrep.2019.08.083 Text en This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Pomrenze, Matthew B.
Giovanetti, Simone M.
Maiya, Rajani
Gordon, Adam G.
Kreeger, Lauren J.
Messing, Robert O.
Dissecting the Roles of GABA and Neuropeptides from Rat Central Amygdala CRF Neurons in Anxiety and Fear Learning
title Dissecting the Roles of GABA and Neuropeptides from Rat Central Amygdala CRF Neurons in Anxiety and Fear Learning
title_full Dissecting the Roles of GABA and Neuropeptides from Rat Central Amygdala CRF Neurons in Anxiety and Fear Learning
title_fullStr Dissecting the Roles of GABA and Neuropeptides from Rat Central Amygdala CRF Neurons in Anxiety and Fear Learning
title_full_unstemmed Dissecting the Roles of GABA and Neuropeptides from Rat Central Amygdala CRF Neurons in Anxiety and Fear Learning
title_short Dissecting the Roles of GABA and Neuropeptides from Rat Central Amygdala CRF Neurons in Anxiety and Fear Learning
title_sort dissecting the roles of gaba and neuropeptides from rat central amygdala crf neurons in anxiety and fear learning
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6879108/
https://www.ncbi.nlm.nih.gov/pubmed/31577943
http://dx.doi.org/10.1016/j.celrep.2019.08.083
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