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Airway microbiome composition correlates with lung function and arterial stiffness in an age-dependent manner

OBJECTIVE: To investigate age-associated changes in airway microbiome composition and their relationships with lung function and arterial stiffness among genetically matched young and elderly pairs. METHODS: Twenty-four genetically linked family pairs comprised of younger (≤40 years) and older (≥60...

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Autores principales: Lee, Shuen Yee, Mac Aogáin, Micheál, Fam, Kai Deng, Chia, Kar Ling, Binte Mohamed Ali, Nur A’tikah, Yap, Margaret M. C., Yap, Eric P. H., Chotirmall, Sanjay H., Lim, Chin Leong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6879132/
https://www.ncbi.nlm.nih.gov/pubmed/31770392
http://dx.doi.org/10.1371/journal.pone.0225636
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author Lee, Shuen Yee
Mac Aogáin, Micheál
Fam, Kai Deng
Chia, Kar Ling
Binte Mohamed Ali, Nur A’tikah
Yap, Margaret M. C.
Yap, Eric P. H.
Chotirmall, Sanjay H.
Lim, Chin Leong
author_facet Lee, Shuen Yee
Mac Aogáin, Micheál
Fam, Kai Deng
Chia, Kar Ling
Binte Mohamed Ali, Nur A’tikah
Yap, Margaret M. C.
Yap, Eric P. H.
Chotirmall, Sanjay H.
Lim, Chin Leong
author_sort Lee, Shuen Yee
collection PubMed
description OBJECTIVE: To investigate age-associated changes in airway microbiome composition and their relationships with lung function and arterial stiffness among genetically matched young and elderly pairs. METHODS: Twenty-four genetically linked family pairs comprised of younger (≤40 years) and older (≥60 years) healthy participants were recruited (Total n = 48). Lung function and arterial stiffness (carotid-femoral pulse wave velocity (PWV) and augmentation index (AIx)) were assessed. Sputum samples were collected for targeted 16S rRNA gene amplicon sequencing and correlations between microbiome composition, lung function and arterial stiffness were investigated. RESULTS: Elderly participants exhibited reductions in lung function (FEV1 (p<0.001), FVC (p<0.001) and percentage FEV1/FVC (p = 0.003)) and a 1.3–3.9-fold increase in arterial stiffness (p<0.001) relative to genetically related younger adults. Elderly adults had a higher relative abundance of Firmicutes (p = 0.035) and lower relative abundance of Proteobacteria (p = 0.014), including specific genera Haemophilus (p = 0.024) and Lautropia (p = 0.020) which were enriched in the younger adults. Alpha diversity was comparable between young and elderly pairs (p>0.05) but was inversely associated with lung function (FEV1%Predicted and FVC %Predicted) in the young (p = 0.006 and p = 0.003) though not the elderly (p = 0.481 and p = 0.696). Conversely, alpha diversity was negatively associated with PWV in the elderly (p = 0.01) but not the young (p = 0.569). Specifically, phylum Firmicutes including the genus Gemella were correlated with lung function (FVC %Predicted) in the young group (p = 0.047 and p = 0.040), while Fusobacteria and Leptotrichia were associated with arterial stiffness (PWV) in the elderly (both p = 0.004). CONCLUSION: Ageing is associated with increased Firmicutes and decreased Proteobacteria representation in the airway microbiome among a healthy Asian cohort. The diversity and composition of the airway microbiome is independently associated with lung function and arterial stiffness in the young and elderly groups respectively. This suggests differential microbial associations with these phenotypes at specific stages of life with potential prognostic implications.
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spelling pubmed-68791322019-12-08 Airway microbiome composition correlates with lung function and arterial stiffness in an age-dependent manner Lee, Shuen Yee Mac Aogáin, Micheál Fam, Kai Deng Chia, Kar Ling Binte Mohamed Ali, Nur A’tikah Yap, Margaret M. C. Yap, Eric P. H. Chotirmall, Sanjay H. Lim, Chin Leong PLoS One Research Article OBJECTIVE: To investigate age-associated changes in airway microbiome composition and their relationships with lung function and arterial stiffness among genetically matched young and elderly pairs. METHODS: Twenty-four genetically linked family pairs comprised of younger (≤40 years) and older (≥60 years) healthy participants were recruited (Total n = 48). Lung function and arterial stiffness (carotid-femoral pulse wave velocity (PWV) and augmentation index (AIx)) were assessed. Sputum samples were collected for targeted 16S rRNA gene amplicon sequencing and correlations between microbiome composition, lung function and arterial stiffness were investigated. RESULTS: Elderly participants exhibited reductions in lung function (FEV1 (p<0.001), FVC (p<0.001) and percentage FEV1/FVC (p = 0.003)) and a 1.3–3.9-fold increase in arterial stiffness (p<0.001) relative to genetically related younger adults. Elderly adults had a higher relative abundance of Firmicutes (p = 0.035) and lower relative abundance of Proteobacteria (p = 0.014), including specific genera Haemophilus (p = 0.024) and Lautropia (p = 0.020) which were enriched in the younger adults. Alpha diversity was comparable between young and elderly pairs (p>0.05) but was inversely associated with lung function (FEV1%Predicted and FVC %Predicted) in the young (p = 0.006 and p = 0.003) though not the elderly (p = 0.481 and p = 0.696). Conversely, alpha diversity was negatively associated with PWV in the elderly (p = 0.01) but not the young (p = 0.569). Specifically, phylum Firmicutes including the genus Gemella were correlated with lung function (FVC %Predicted) in the young group (p = 0.047 and p = 0.040), while Fusobacteria and Leptotrichia were associated with arterial stiffness (PWV) in the elderly (both p = 0.004). CONCLUSION: Ageing is associated with increased Firmicutes and decreased Proteobacteria representation in the airway microbiome among a healthy Asian cohort. The diversity and composition of the airway microbiome is independently associated with lung function and arterial stiffness in the young and elderly groups respectively. This suggests differential microbial associations with these phenotypes at specific stages of life with potential prognostic implications. Public Library of Science 2019-11-26 /pmc/articles/PMC6879132/ /pubmed/31770392 http://dx.doi.org/10.1371/journal.pone.0225636 Text en © 2019 Lee et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Lee, Shuen Yee
Mac Aogáin, Micheál
Fam, Kai Deng
Chia, Kar Ling
Binte Mohamed Ali, Nur A’tikah
Yap, Margaret M. C.
Yap, Eric P. H.
Chotirmall, Sanjay H.
Lim, Chin Leong
Airway microbiome composition correlates with lung function and arterial stiffness in an age-dependent manner
title Airway microbiome composition correlates with lung function and arterial stiffness in an age-dependent manner
title_full Airway microbiome composition correlates with lung function and arterial stiffness in an age-dependent manner
title_fullStr Airway microbiome composition correlates with lung function and arterial stiffness in an age-dependent manner
title_full_unstemmed Airway microbiome composition correlates with lung function and arterial stiffness in an age-dependent manner
title_short Airway microbiome composition correlates with lung function and arterial stiffness in an age-dependent manner
title_sort airway microbiome composition correlates with lung function and arterial stiffness in an age-dependent manner
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6879132/
https://www.ncbi.nlm.nih.gov/pubmed/31770392
http://dx.doi.org/10.1371/journal.pone.0225636
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