Cargando…

PIPE-T: a new Galaxy tool for the analysis of RT-qPCR expression data

Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) is an accurate and fast method to measure gene expression. Reproducibility of the analyses is the main limitation of RT-qPCR experiments. Galaxy is an open, web-based, genomic workbench for a reproducible, transparent,...

Descripción completa

Detalles Bibliográficos
Autores principales:	Zanardi, Nicolò, Morini, Martina, Tangaro, Marco Antonio, Zambelli, Federico, Bosco, Maria Carla, Varesio, Luigi, Eva, Alessandra, Cangelosi, Davide
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2019
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6879478/ https://www.ncbi.nlm.nih.gov/pubmed/31772190 http://dx.doi.org/10.1038/s41598-019-53155-9

Ejemplares similares

Hypoxia Predicts Poor Prognosis in Neuroblastoma Patients and Associates with Biological Mechanisms Involved in Telomerase Activation and Tumor Microenvironment Reprogramming
por: Cangelosi, Davide, et al.
Publicado: (2020)

Tutorial: Guidelines for Single-Cell RT-qPCR
por: Zucha, Daniel, et al.
Publicado: (2021)

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR
por: Fout, G. Shay, et al.
Publicado: (2016)

Algorithm Optimization in Methylation Detection with Multiple RT-qPCR
por: Song, Lele, et al.
Publicado: (2016)

Improving the standardization of mRNA measurement by RT-qPCR
por: Sanders, Rebecca, et al.
Publicado: (2018)

COVID-19 diagnosis by RT-qPCR in alternative specimens
por: Gonçalves, Cássia Cristina Alves, et al.
Publicado: (2021)

Rapid and accurate quantification of isomiRs by RT-qPCR
por: Franco, Sandra, et al.
Publicado: (2022)

Evaluation of the mechanism of action of Bacillus spp. to manage Meloidogyne incognita with split root assay, RT-qPCR and qPCR
por: Gattoni, Kaitlin M., et al.
Publicado: (2023)

Development of RT-qPCR and semi-nested RT-PCR assays for molecular diagnosis of hantavirus pulmonary syndrome
por: Nunes, Bruno Tardelli Diniz, et al.
Publicado: (2019)

SASqPCR: Robust and Rapid Analysis of RT-qPCR Data in SAS
por: Ling, Daijun
Publicado: (2012)

Comparison of RT-qPCR and RT-ddPCR with Rift valley fever virus (RVFV) RNA
por: Park, Changwoo, et al.
Publicado: (2023)

Artificial neural network classifier predicts neuroblastoma patients’ outcome
por: Cangelosi, Davide, et al.
Publicado: (2016)

Immunohistochemical analysis of PDK1, PHD3 and HIF-1α expression defines the hypoxic status of neuroblastoma tumors
por: Ognibene, Marzia, et al.
Publicado: (2017)

Evaluation and validation of reference genes for RT-qPCR gene expression in Naegleria gruberi
por: Martín-Pérez, Tania, et al.
Publicado: (2023)

Validation of reference genes for the normalization of RT-qPCR gene expression in Acanthamoeba spp.
por: Köhsler, Martina, et al.
Publicado: (2020)

Detection and identification of potentially infectious gastrointestinal and respiratory viruses at workplaces of wastewater treatment plants with viability qPCR/RT-qPCR
por: Stobnicka-Kupiec, Agata, et al.
Publicado: (2022)

Selection of Suitable Reference Genes for RT-qPCR Analyses in Cyanobacteria
por: Pinto, Filipe, et al.
Publicado: (2012)

Applicability of RNA standards for evaluating RT-qPCR assays and platforms
por: Devonshire, Alison S, et al.
Publicado: (2011)

RT-qPCR Normalization Genes in the Red Alga Chondrus crispus
por: Kowalczyk, Nathalie, et al.
Publicado: (2014)

Hepatitis A virus subgenotyping based on RT-qPCR assays
por: Coudray-Meunier, Coralie, et al.
Publicado: (2014)

Custom RT-qPCR-array for glaucoma filtering surgery prognosis
por: Rodriguez-Agirretxe, Iñaki, et al.
Publicado: (2017)

Linear-hairpin variable primer RT-qPCR for MicroRNA
por: Lan, Lin, et al.
Publicado: (2018)

Predictive factors of COVID-19 in patients with negative RT-qPCR
por: López de la Iglesia, J., et al.
Publicado: (2020)

Large-Scale RT-qPCR Diagnostics for Seed Potato Certification
por: Schumpp, Olivier, et al.
Publicado: (2021)

Sampling, Logistics, and Analytics of Urine for RT-qPCR-based Diagnostics
por: Kretschmer-Kazemi Far, Rosel, et al.
Publicado: (2021)

RT-qPCR-based pool testing for the diagnosis of COVID-19
por: Sato, Hugo Itaru, et al.
Publicado: (2023)

Detection of Cytoplasmic and Nuclear Circular RNA via RT-qPCR
por: Tan, Ke-En, et al.
Publicado: (2023)

NormQ: RNASeq normalization based on RT-qPCR derived size factors
por: Naraine, Ravindra, et al.
Publicado: (2020)

Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies
por: Paiva, Marcelo Henrique Santos, et al.
Publicado: (2017)

Assessing sensitivity and reproducibility of RT-ddPCR and RT-qPCR for the quantification of SARS-CoV-2 in wastewater
por: Ciesielski, Mark, et al.
Publicado: (2021)

Mobile Efficient Diagnostics of Infectious Diseases via On‐Chip RT‐qPCR: MEDIC‐PCR
por: Shrestha, Kiran, et al.
Publicado: (2023)

Reverse transcription-quantitative PCR (RT-qPCR) without the need for prior removal of DNA
por: Đermić, Damir, et al.
Publicado: (2023)

Accurate RT-qPCR gene expression analysis on cell culture lysates
por: Van Peer, Gert, et al.
Publicado: (2012)

A reliable method for quantification of splice variants using RT-qPCR
por: Camacho Londoño, Julia, et al.
Publicado: (2016)

Assessment of brain reference genes for RT-qPCR studies in neurodegenerative diseases
por: Rydbirk, Rasmus, et al.
Publicado: (2016)

Assessment of suitable reference genes for RT–qPCR studies in chronic rhinosinusitis
por: Nakayama, Tsuguhisa, et al.
Publicado: (2018)

Eprobe mediated RT-qPCR for the detection of leukemia-associated fusion genes
por: Tsuchiya, Koji, et al.
Publicado: (2018)

Detection of SARS-CoV-2 RNA by multiplex RT-qPCR
por: Kudo, Eriko, et al.
Publicado: (2020)

RT-qPCR Testing of SARS-CoV-2: A Primer
por: Bustin, Stephen A., et al.
Publicado: (2020)

Identification of reference genes for RT-qPCR data normalisation in aging studies
por: González-Bermúdez, Lourdes, et al.
Publicado: (2019)

Cannot write session to /tmp/vufind_sessions/sess_kh77enj71iev500rq9vgdd0ukj