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LncRNA BANCR Promotes Pancreatic Cancer Tumorigenesis via Modulating MiR-195-5p/Wnt/β-Catenin Signaling Pathway

Long noncoding BRAF-activated noncoding RNA has been reported to be tightly associated with tumorigenesis and development in various types of cancers. However, the expression, biological function, and modulatory mechanism of BRAF-activated noncoding RNA in pancreatic cancer remained unclear. In the...

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Autores principales: Wu, Xinquan, Xia, Tianfang, Cao, Meng, Zhang, Pengbo, Shi, Guodong, Chen, Lei, Zhang, Jingjing, Yin, Jie, Wu, Pengfei, Cai, Baobao, Lu, Zipeng, Miao, Yi, Jiang, Kuirong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6880033/
https://www.ncbi.nlm.nih.gov/pubmed/31769353
http://dx.doi.org/10.1177/1533033819887962
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author Wu, Xinquan
Xia, Tianfang
Cao, Meng
Zhang, Pengbo
Shi, Guodong
Chen, Lei
Zhang, Jingjing
Yin, Jie
Wu, Pengfei
Cai, Baobao
Lu, Zipeng
Miao, Yi
Jiang, Kuirong
author_facet Wu, Xinquan
Xia, Tianfang
Cao, Meng
Zhang, Pengbo
Shi, Guodong
Chen, Lei
Zhang, Jingjing
Yin, Jie
Wu, Pengfei
Cai, Baobao
Lu, Zipeng
Miao, Yi
Jiang, Kuirong
author_sort Wu, Xinquan
collection PubMed
description Long noncoding BRAF-activated noncoding RNA has been reported to be tightly associated with tumorigenesis and development in various types of cancers. However, the expression, biological function, and modulatory mechanism of BRAF-activated noncoding RNA in pancreatic cancer remained unclear. In the present work, we explored the carcinogenic activity and underlying mechanism of BRAF-activated noncoding RNA on pancreatic cancer in vitro. We identified that BRAF-activated noncoding RNA was upregulated in pancreatic cancer tissues and cell lines, and BRAF-activated noncoding RNA was related to tumor metastasis and stage. BRAF-activated noncoding RNA reinforces proliferation, invasion, and migration in PANC-1 and SW1990 cells. Moreover, miR-195-5p was downregulated in both PC tissues and cell lines. Our results based on luciferase reporter, RIP-Ago2 and qRT-PCR assays, showed that miR-195-5p was a direct target of BRAF-activated noncoding RNA. Furthermore, miR-195-5p inhibitor abrogated the effects of short-interfering BRAF-activated noncoding RNA on PANC-1 and SW1990 cell growth and invasion in vitro. We further identified that BRAF-activated noncoding RNA played a vital role in activating the Wnt/β-catenin pathway by sponging miR-195-5p. Collectively, our study showed that BRAF-activated noncoding RNA promotes pancreatic cancer tumorigenesis through miR-195-5p/Wnt/β-catenin axis may serve as a potential target for diagnostics and therapeutics in pancreatic cancer.
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spelling pubmed-68800332019-12-05 LncRNA BANCR Promotes Pancreatic Cancer Tumorigenesis via Modulating MiR-195-5p/Wnt/β-Catenin Signaling Pathway Wu, Xinquan Xia, Tianfang Cao, Meng Zhang, Pengbo Shi, Guodong Chen, Lei Zhang, Jingjing Yin, Jie Wu, Pengfei Cai, Baobao Lu, Zipeng Miao, Yi Jiang, Kuirong Technol Cancer Res Treat Original Article Long noncoding BRAF-activated noncoding RNA has been reported to be tightly associated with tumorigenesis and development in various types of cancers. However, the expression, biological function, and modulatory mechanism of BRAF-activated noncoding RNA in pancreatic cancer remained unclear. In the present work, we explored the carcinogenic activity and underlying mechanism of BRAF-activated noncoding RNA on pancreatic cancer in vitro. We identified that BRAF-activated noncoding RNA was upregulated in pancreatic cancer tissues and cell lines, and BRAF-activated noncoding RNA was related to tumor metastasis and stage. BRAF-activated noncoding RNA reinforces proliferation, invasion, and migration in PANC-1 and SW1990 cells. Moreover, miR-195-5p was downregulated in both PC tissues and cell lines. Our results based on luciferase reporter, RIP-Ago2 and qRT-PCR assays, showed that miR-195-5p was a direct target of BRAF-activated noncoding RNA. Furthermore, miR-195-5p inhibitor abrogated the effects of short-interfering BRAF-activated noncoding RNA on PANC-1 and SW1990 cell growth and invasion in vitro. We further identified that BRAF-activated noncoding RNA played a vital role in activating the Wnt/β-catenin pathway by sponging miR-195-5p. Collectively, our study showed that BRAF-activated noncoding RNA promotes pancreatic cancer tumorigenesis through miR-195-5p/Wnt/β-catenin axis may serve as a potential target for diagnostics and therapeutics in pancreatic cancer. SAGE Publications 2019-11-26 /pmc/articles/PMC6880033/ /pubmed/31769353 http://dx.doi.org/10.1177/1533033819887962 Text en © The Author(s) 2019 http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Article
Wu, Xinquan
Xia, Tianfang
Cao, Meng
Zhang, Pengbo
Shi, Guodong
Chen, Lei
Zhang, Jingjing
Yin, Jie
Wu, Pengfei
Cai, Baobao
Lu, Zipeng
Miao, Yi
Jiang, Kuirong
LncRNA BANCR Promotes Pancreatic Cancer Tumorigenesis via Modulating MiR-195-5p/Wnt/β-Catenin Signaling Pathway
title LncRNA BANCR Promotes Pancreatic Cancer Tumorigenesis via Modulating MiR-195-5p/Wnt/β-Catenin Signaling Pathway
title_full LncRNA BANCR Promotes Pancreatic Cancer Tumorigenesis via Modulating MiR-195-5p/Wnt/β-Catenin Signaling Pathway
title_fullStr LncRNA BANCR Promotes Pancreatic Cancer Tumorigenesis via Modulating MiR-195-5p/Wnt/β-Catenin Signaling Pathway
title_full_unstemmed LncRNA BANCR Promotes Pancreatic Cancer Tumorigenesis via Modulating MiR-195-5p/Wnt/β-Catenin Signaling Pathway
title_short LncRNA BANCR Promotes Pancreatic Cancer Tumorigenesis via Modulating MiR-195-5p/Wnt/β-Catenin Signaling Pathway
title_sort lncrna bancr promotes pancreatic cancer tumorigenesis via modulating mir-195-5p/wnt/β-catenin signaling pathway
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6880033/
https://www.ncbi.nlm.nih.gov/pubmed/31769353
http://dx.doi.org/10.1177/1533033819887962
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