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HAX1 impact on collective cell migration, cell adhesion, and cell shape is linked to the regulation of actomyosin contractility
HAX1 protein is involved in the regulation of apoptosis, cell motility and calcium homeostasis. Its overexpression was reported in several tumors, including breast cancer. This study demonstrates that HAX1 has an impact on collective, but not single-cell migration, thus indicating the importance of...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6880882/ https://www.ncbi.nlm.nih.gov/pubmed/31644363 http://dx.doi.org/10.1091/mbc.E19-05-0304 |
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author | Balcerak, Anna Trebinska-Stryjewska, Alicja Wakula, Maciej Chmielarczyk, Mateusz Smietanka, Urszula Rubel, Tymon Konopinski, Ryszard Macech-Klicka, Ewelina Zub, Renata Grzybowska, Ewa Anna |
author_facet | Balcerak, Anna Trebinska-Stryjewska, Alicja Wakula, Maciej Chmielarczyk, Mateusz Smietanka, Urszula Rubel, Tymon Konopinski, Ryszard Macech-Klicka, Ewelina Zub, Renata Grzybowska, Ewa Anna |
author_sort | Balcerak, Anna |
collection | PubMed |
description | HAX1 protein is involved in the regulation of apoptosis, cell motility and calcium homeostasis. Its overexpression was reported in several tumors, including breast cancer. This study demonstrates that HAX1 has an impact on collective, but not single-cell migration, thus indicating the importance of cell–cell contacts for the HAX1-mediated effect. Accordingly, it was shown that HAX1 knockdown affects cell–cell junctions, substrate adhesion, and epithelial cell layer integrity. As demonstrated here, these effects can be attributed to the modulation of actomyosin contractility through changes in RhoA and septin signaling. Additionally, it was shown that HAX1 does not influence invasive potential in the breast cancer cell line, suggesting that its role in breast cancer progression may be linked instead to collective invasion of the epithelial cells but not single-cell dissemination. |
format | Online Article Text |
id | pubmed-6880882 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-68808822020-02-16 HAX1 impact on collective cell migration, cell adhesion, and cell shape is linked to the regulation of actomyosin contractility Balcerak, Anna Trebinska-Stryjewska, Alicja Wakula, Maciej Chmielarczyk, Mateusz Smietanka, Urszula Rubel, Tymon Konopinski, Ryszard Macech-Klicka, Ewelina Zub, Renata Grzybowska, Ewa Anna Mol Biol Cell Articles HAX1 protein is involved in the regulation of apoptosis, cell motility and calcium homeostasis. Its overexpression was reported in several tumors, including breast cancer. This study demonstrates that HAX1 has an impact on collective, but not single-cell migration, thus indicating the importance of cell–cell contacts for the HAX1-mediated effect. Accordingly, it was shown that HAX1 knockdown affects cell–cell junctions, substrate adhesion, and epithelial cell layer integrity. As demonstrated here, these effects can be attributed to the modulation of actomyosin contractility through changes in RhoA and septin signaling. Additionally, it was shown that HAX1 does not influence invasive potential in the breast cancer cell line, suggesting that its role in breast cancer progression may be linked instead to collective invasion of the epithelial cells but not single-cell dissemination. The American Society for Cell Biology 2019-12-01 /pmc/articles/PMC6880882/ /pubmed/31644363 http://dx.doi.org/10.1091/mbc.E19-05-0304 Text en © 2019 Balcerak, Trebinska-Stryjewska, et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. http://creativecommons.org/licenses/by-nc-sa/3.0 This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License. |
spellingShingle | Articles Balcerak, Anna Trebinska-Stryjewska, Alicja Wakula, Maciej Chmielarczyk, Mateusz Smietanka, Urszula Rubel, Tymon Konopinski, Ryszard Macech-Klicka, Ewelina Zub, Renata Grzybowska, Ewa Anna HAX1 impact on collective cell migration, cell adhesion, and cell shape is linked to the regulation of actomyosin contractility |
title | HAX1 impact on collective cell migration, cell adhesion, and cell shape is linked to the regulation of actomyosin contractility |
title_full | HAX1 impact on collective cell migration, cell adhesion, and cell shape is linked to the regulation of actomyosin contractility |
title_fullStr | HAX1 impact on collective cell migration, cell adhesion, and cell shape is linked to the regulation of actomyosin contractility |
title_full_unstemmed | HAX1 impact on collective cell migration, cell adhesion, and cell shape is linked to the regulation of actomyosin contractility |
title_short | HAX1 impact on collective cell migration, cell adhesion, and cell shape is linked to the regulation of actomyosin contractility |
title_sort | hax1 impact on collective cell migration, cell adhesion, and cell shape is linked to the regulation of actomyosin contractility |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6880882/ https://www.ncbi.nlm.nih.gov/pubmed/31644363 http://dx.doi.org/10.1091/mbc.E19-05-0304 |
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