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In vitro ovicidal and larvicidal activity of Carica papaya seed hexane extract against Strongyloides venezuelensis
Strongyloidiasis is a human parasitic disease caused by the helminth Strongyloides stercoralis whose treatment is particularly difficult in immunosuppressed patients due to their low responsiveness to conventional therapy. Carica papaya and its isolated compounds benzyl isothiocyanate, carpaine and...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Instituto de Medicina Tropical
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6880996/ https://www.ncbi.nlm.nih.gov/pubmed/31778389 http://dx.doi.org/10.1590/S1678-9946201961059 |
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author | Cabral, Eduardo Ramos Martins Moraes, Dayane Levenhagen, Marcelo Arantes de Matos, Ricardo Alexandre Figueiredo Costa-Cruz, Julia Maria Rodrigues, Rosângela Maria |
author_facet | Cabral, Eduardo Ramos Martins Moraes, Dayane Levenhagen, Marcelo Arantes de Matos, Ricardo Alexandre Figueiredo Costa-Cruz, Julia Maria Rodrigues, Rosângela Maria |
author_sort | Cabral, Eduardo Ramos Martins |
collection | PubMed |
description | Strongyloidiasis is a human parasitic disease caused by the helminth Strongyloides stercoralis whose treatment is particularly difficult in immunosuppressed patients due to their low responsiveness to conventional therapy. Carica papaya and its isolated compounds benzyl isothiocyanate, carpaine and carpasemine are promising compound for the treatment of Strongyloides infections due to their anthelmintic action. This study aims to examine the in vitro ovicidal and larvicidal activity of C. papaya seed hexane extract against Strongyloides venezuelensis, using egg hatching tests and larval motility tests as efficiency markers. The crude extract at the concentrations of 566 – 0.0566 mg/mL or the control with albendazole (0.025 mg/mL) and negative controls (water and PBS) were incubated with an equal volume of egg suspension (± 50 specimens) followed by counting of the specimens after 48 h. The same extract and dilutions were added to L3 larvae suspensions (±50 specimens) followed by analysis of larvae viability after 24, 48, and 72 h. The extract inhibited egg hatching with high efficiency at concentrations of 56.6 mg/mL (95.74%) and 5.66 mg/mL (92.16%). At the concentrations of 566 mg/mL (100%) and 56.66 mg/mL (97.32%), the extract inhibited larval motility as effectively as ivermectin (0.316 mg/mL; 100%), and more effectively than the other dilutions and the negative controls. The larvicidal effect depended on the extract concentration, but not on the treatment period. Therefore, C. papaya seed hexane extract has anthelmintic potential against S. venezuelensis and is a promising compound for the development of phytotherapies to treat strongyloidiasis. |
format | Online Article Text |
id | pubmed-6880996 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Instituto de Medicina Tropical |
record_format | MEDLINE/PubMed |
spelling | pubmed-68809962019-12-05 In vitro ovicidal and larvicidal activity of Carica papaya seed hexane extract against Strongyloides venezuelensis Cabral, Eduardo Ramos Martins Moraes, Dayane Levenhagen, Marcelo Arantes de Matos, Ricardo Alexandre Figueiredo Costa-Cruz, Julia Maria Rodrigues, Rosângela Maria Rev Inst Med Trop Sao Paulo Original Article Strongyloidiasis is a human parasitic disease caused by the helminth Strongyloides stercoralis whose treatment is particularly difficult in immunosuppressed patients due to their low responsiveness to conventional therapy. Carica papaya and its isolated compounds benzyl isothiocyanate, carpaine and carpasemine are promising compound for the treatment of Strongyloides infections due to their anthelmintic action. This study aims to examine the in vitro ovicidal and larvicidal activity of C. papaya seed hexane extract against Strongyloides venezuelensis, using egg hatching tests and larval motility tests as efficiency markers. The crude extract at the concentrations of 566 – 0.0566 mg/mL or the control with albendazole (0.025 mg/mL) and negative controls (water and PBS) were incubated with an equal volume of egg suspension (± 50 specimens) followed by counting of the specimens after 48 h. The same extract and dilutions were added to L3 larvae suspensions (±50 specimens) followed by analysis of larvae viability after 24, 48, and 72 h. The extract inhibited egg hatching with high efficiency at concentrations of 56.6 mg/mL (95.74%) and 5.66 mg/mL (92.16%). At the concentrations of 566 mg/mL (100%) and 56.66 mg/mL (97.32%), the extract inhibited larval motility as effectively as ivermectin (0.316 mg/mL; 100%), and more effectively than the other dilutions and the negative controls. The larvicidal effect depended on the extract concentration, but not on the treatment period. Therefore, C. papaya seed hexane extract has anthelmintic potential against S. venezuelensis and is a promising compound for the development of phytotherapies to treat strongyloidiasis. Instituto de Medicina Tropical 2019-11-25 /pmc/articles/PMC6880996/ /pubmed/31778389 http://dx.doi.org/10.1590/S1678-9946201961059 Text en https://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Cabral, Eduardo Ramos Martins Moraes, Dayane Levenhagen, Marcelo Arantes de Matos, Ricardo Alexandre Figueiredo Costa-Cruz, Julia Maria Rodrigues, Rosângela Maria In vitro ovicidal and larvicidal activity of Carica papaya seed hexane extract against Strongyloides venezuelensis |
title | In vitro ovicidal and larvicidal activity of Carica papaya seed
hexane extract against Strongyloides venezuelensis |
title_full | In vitro ovicidal and larvicidal activity of Carica papaya seed
hexane extract against Strongyloides venezuelensis |
title_fullStr | In vitro ovicidal and larvicidal activity of Carica papaya seed
hexane extract against Strongyloides venezuelensis |
title_full_unstemmed | In vitro ovicidal and larvicidal activity of Carica papaya seed
hexane extract against Strongyloides venezuelensis |
title_short | In vitro ovicidal and larvicidal activity of Carica papaya seed
hexane extract against Strongyloides venezuelensis |
title_sort | in vitro ovicidal and larvicidal activity of carica papaya seed
hexane extract against strongyloides venezuelensis |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6880996/ https://www.ncbi.nlm.nih.gov/pubmed/31778389 http://dx.doi.org/10.1590/S1678-9946201961059 |
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