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In vitro ovicidal and larvicidal activity of Carica papaya seed hexane extract against Strongyloides venezuelensis

Strongyloidiasis is a human parasitic disease caused by the helminth Strongyloides stercoralis whose treatment is particularly difficult in immunosuppressed patients due to their low responsiveness to conventional therapy. Carica papaya and its isolated compounds benzyl isothiocyanate, carpaine and...

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Autores principales: Cabral, Eduardo Ramos Martins, Moraes, Dayane, Levenhagen, Marcelo Arantes, de Matos, Ricardo Alexandre Figueiredo, Costa-Cruz, Julia Maria, Rodrigues, Rosângela Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto de Medicina Tropical 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6880996/
https://www.ncbi.nlm.nih.gov/pubmed/31778389
http://dx.doi.org/10.1590/S1678-9946201961059
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author Cabral, Eduardo Ramos Martins
Moraes, Dayane
Levenhagen, Marcelo Arantes
de Matos, Ricardo Alexandre Figueiredo
Costa-Cruz, Julia Maria
Rodrigues, Rosângela Maria
author_facet Cabral, Eduardo Ramos Martins
Moraes, Dayane
Levenhagen, Marcelo Arantes
de Matos, Ricardo Alexandre Figueiredo
Costa-Cruz, Julia Maria
Rodrigues, Rosângela Maria
author_sort Cabral, Eduardo Ramos Martins
collection PubMed
description Strongyloidiasis is a human parasitic disease caused by the helminth Strongyloides stercoralis whose treatment is particularly difficult in immunosuppressed patients due to their low responsiveness to conventional therapy. Carica papaya and its isolated compounds benzyl isothiocyanate, carpaine and carpasemine are promising compound for the treatment of Strongyloides infections due to their anthelmintic action. This study aims to examine the in vitro ovicidal and larvicidal activity of C. papaya seed hexane extract against Strongyloides venezuelensis, using egg hatching tests and larval motility tests as efficiency markers. The crude extract at the concentrations of 566 – 0.0566 mg/mL or the control with albendazole (0.025 mg/mL) and negative controls (water and PBS) were incubated with an equal volume of egg suspension (± 50 specimens) followed by counting of the specimens after 48 h. The same extract and dilutions were added to L3 larvae suspensions (±50 specimens) followed by analysis of larvae viability after 24, 48, and 72 h. The extract inhibited egg hatching with high efficiency at concentrations of 56.6 mg/mL (95.74%) and 5.66 mg/mL (92.16%). At the concentrations of 566 mg/mL (100%) and 56.66 mg/mL (97.32%), the extract inhibited larval motility as effectively as ivermectin (0.316 mg/mL; 100%), and more effectively than the other dilutions and the negative controls. The larvicidal effect depended on the extract concentration, but not on the treatment period. Therefore, C. papaya seed hexane extract has anthelmintic potential against S. venezuelensis and is a promising compound for the development of phytotherapies to treat strongyloidiasis.
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spelling pubmed-68809962019-12-05 In vitro ovicidal and larvicidal activity of Carica papaya seed hexane extract against Strongyloides venezuelensis Cabral, Eduardo Ramos Martins Moraes, Dayane Levenhagen, Marcelo Arantes de Matos, Ricardo Alexandre Figueiredo Costa-Cruz, Julia Maria Rodrigues, Rosângela Maria Rev Inst Med Trop Sao Paulo Original Article Strongyloidiasis is a human parasitic disease caused by the helminth Strongyloides stercoralis whose treatment is particularly difficult in immunosuppressed patients due to their low responsiveness to conventional therapy. Carica papaya and its isolated compounds benzyl isothiocyanate, carpaine and carpasemine are promising compound for the treatment of Strongyloides infections due to their anthelmintic action. This study aims to examine the in vitro ovicidal and larvicidal activity of C. papaya seed hexane extract against Strongyloides venezuelensis, using egg hatching tests and larval motility tests as efficiency markers. The crude extract at the concentrations of 566 – 0.0566 mg/mL or the control with albendazole (0.025 mg/mL) and negative controls (water and PBS) were incubated with an equal volume of egg suspension (± 50 specimens) followed by counting of the specimens after 48 h. The same extract and dilutions were added to L3 larvae suspensions (±50 specimens) followed by analysis of larvae viability after 24, 48, and 72 h. The extract inhibited egg hatching with high efficiency at concentrations of 56.6 mg/mL (95.74%) and 5.66 mg/mL (92.16%). At the concentrations of 566 mg/mL (100%) and 56.66 mg/mL (97.32%), the extract inhibited larval motility as effectively as ivermectin (0.316 mg/mL; 100%), and more effectively than the other dilutions and the negative controls. The larvicidal effect depended on the extract concentration, but not on the treatment period. Therefore, C. papaya seed hexane extract has anthelmintic potential against S. venezuelensis and is a promising compound for the development of phytotherapies to treat strongyloidiasis. Instituto de Medicina Tropical 2019-11-25 /pmc/articles/PMC6880996/ /pubmed/31778389 http://dx.doi.org/10.1590/S1678-9946201961059 Text en https://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Cabral, Eduardo Ramos Martins
Moraes, Dayane
Levenhagen, Marcelo Arantes
de Matos, Ricardo Alexandre Figueiredo
Costa-Cruz, Julia Maria
Rodrigues, Rosângela Maria
In vitro ovicidal and larvicidal activity of Carica papaya seed hexane extract against Strongyloides venezuelensis
title In vitro ovicidal and larvicidal activity of Carica papaya seed hexane extract against Strongyloides venezuelensis
title_full In vitro ovicidal and larvicidal activity of Carica papaya seed hexane extract against Strongyloides venezuelensis
title_fullStr In vitro ovicidal and larvicidal activity of Carica papaya seed hexane extract against Strongyloides venezuelensis
title_full_unstemmed In vitro ovicidal and larvicidal activity of Carica papaya seed hexane extract against Strongyloides venezuelensis
title_short In vitro ovicidal and larvicidal activity of Carica papaya seed hexane extract against Strongyloides venezuelensis
title_sort in vitro ovicidal and larvicidal activity of carica papaya seed hexane extract against strongyloides venezuelensis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6880996/
https://www.ncbi.nlm.nih.gov/pubmed/31778389
http://dx.doi.org/10.1590/S1678-9946201961059
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