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Comparison of the Cardiomyogenic Potency of Human Amniotic Fluid and Bone Marrow Mesenchymal Stem Cells
BACKGROUND AND OBJECTIVES: Most studies in cardiac regeneration have explored bone marrow mesenchymal stem cells (BM-MSC) with variable therapeutic effects. Amniotic fluid MSC (AF-MSC) having extended self-renewal and multipotent properties may be superior to bone marrow MSC (BM-MSC). However, a com...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Korean Society for Stem Cell Research
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6881043/ https://www.ncbi.nlm.nih.gov/pubmed/31658508 http://dx.doi.org/10.15283/ijsc18087 |
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author | Jain, Manali Minocha, Ekta Tripathy, Naresh Kumar Singh, Neeta Chaturvedi, Chandra Prakash Nityanand, Soniya |
author_facet | Jain, Manali Minocha, Ekta Tripathy, Naresh Kumar Singh, Neeta Chaturvedi, Chandra Prakash Nityanand, Soniya |
author_sort | Jain, Manali |
collection | PubMed |
description | BACKGROUND AND OBJECTIVES: Most studies in cardiac regeneration have explored bone marrow mesenchymal stem cells (BM-MSC) with variable therapeutic effects. Amniotic fluid MSC (AF-MSC) having extended self-renewal and multipotent properties may be superior to bone marrow MSC (BM-MSC). However, a comparison of their cardiomyogenic potency has not been studied yet. METHODS: The 5-azacytidine (5-aza) treated AF-MSC and BM-MSC were evaluated for the expression of GATA-4, Nkx2.5 and ISL-1 transcripts and proteins by quantitative RT-PCR and Western blotting, respectively as well as for the expression of cardiomyogenic differentiation markers cardiac troponin-T (cTNT), beta myosin heavy chain (βMHC) and alpha sarcomeric actinin (ASA) by immunocytochemistry. RESULTS: The AF-MSC as compared to BM-MSC had significantly higher expression of GATA-4 (183.06±29.85 vs. 9.80±0.05; p<0.01), Nkx2.5 (8.3±1.4 vs. 1.82±0.32; p<0.05), and ISL-1 (39.59±4.05 vs. 4.36±0.39; p<0.01) genes as well as GATA-4 (2.01±0.5 vs. 0.6±0.1; p<0.05), NKx2.5 (1.9±0.14 vs. 0.8±0.2; p<0.01) and ISL-1 (1.7±0.3 vs. 0.9±0.1; p<0.05) proteins. The AF-MSC also had significantly elevated expression of cTNT (5.0×10(4)±0.6×10(4) vs. 3.5 ×10(4)±0.8×10(4); p<0.01), β-MHC (15.7×10(4)±0.9×10(4) vs. 8.2×10(4)±0.6×10(4); p<0.01) and ASA (18.6×10(4)±4.9×10(4) vs. 13.1×10(4)±3.0×10(4); p<0.05) than BM-MSC. CONCLUSIONS: Our data suggest that AF-MSC have greater cardiomyogenic potency than BM-MSC, and thus may be a better source of MSC for therapeutic applications in cardiac regenerative medicine. |
format | Online Article Text |
id | pubmed-6881043 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Korean Society for Stem Cell Research |
record_format | MEDLINE/PubMed |
spelling | pubmed-68810432019-12-05 Comparison of the Cardiomyogenic Potency of Human Amniotic Fluid and Bone Marrow Mesenchymal Stem Cells Jain, Manali Minocha, Ekta Tripathy, Naresh Kumar Singh, Neeta Chaturvedi, Chandra Prakash Nityanand, Soniya Int J Stem Cells Brief Report BACKGROUND AND OBJECTIVES: Most studies in cardiac regeneration have explored bone marrow mesenchymal stem cells (BM-MSC) with variable therapeutic effects. Amniotic fluid MSC (AF-MSC) having extended self-renewal and multipotent properties may be superior to bone marrow MSC (BM-MSC). However, a comparison of their cardiomyogenic potency has not been studied yet. METHODS: The 5-azacytidine (5-aza) treated AF-MSC and BM-MSC were evaluated for the expression of GATA-4, Nkx2.5 and ISL-1 transcripts and proteins by quantitative RT-PCR and Western blotting, respectively as well as for the expression of cardiomyogenic differentiation markers cardiac troponin-T (cTNT), beta myosin heavy chain (βMHC) and alpha sarcomeric actinin (ASA) by immunocytochemistry. RESULTS: The AF-MSC as compared to BM-MSC had significantly higher expression of GATA-4 (183.06±29.85 vs. 9.80±0.05; p<0.01), Nkx2.5 (8.3±1.4 vs. 1.82±0.32; p<0.05), and ISL-1 (39.59±4.05 vs. 4.36±0.39; p<0.01) genes as well as GATA-4 (2.01±0.5 vs. 0.6±0.1; p<0.05), NKx2.5 (1.9±0.14 vs. 0.8±0.2; p<0.01) and ISL-1 (1.7±0.3 vs. 0.9±0.1; p<0.05) proteins. The AF-MSC also had significantly elevated expression of cTNT (5.0×10(4)±0.6×10(4) vs. 3.5 ×10(4)±0.8×10(4); p<0.01), β-MHC (15.7×10(4)±0.9×10(4) vs. 8.2×10(4)±0.6×10(4); p<0.01) and ASA (18.6×10(4)±4.9×10(4) vs. 13.1×10(4)±3.0×10(4); p<0.05) than BM-MSC. CONCLUSIONS: Our data suggest that AF-MSC have greater cardiomyogenic potency than BM-MSC, and thus may be a better source of MSC for therapeutic applications in cardiac regenerative medicine. Korean Society for Stem Cell Research 2019-10-31 /pmc/articles/PMC6881043/ /pubmed/31658508 http://dx.doi.org/10.15283/ijsc18087 Text en Copyright © 2019 by the Korean Society for Stem Cell Research https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Brief Report Jain, Manali Minocha, Ekta Tripathy, Naresh Kumar Singh, Neeta Chaturvedi, Chandra Prakash Nityanand, Soniya Comparison of the Cardiomyogenic Potency of Human Amniotic Fluid and Bone Marrow Mesenchymal Stem Cells |
title | Comparison of the Cardiomyogenic Potency of Human Amniotic Fluid and Bone Marrow Mesenchymal Stem Cells |
title_full | Comparison of the Cardiomyogenic Potency of Human Amniotic Fluid and Bone Marrow Mesenchymal Stem Cells |
title_fullStr | Comparison of the Cardiomyogenic Potency of Human Amniotic Fluid and Bone Marrow Mesenchymal Stem Cells |
title_full_unstemmed | Comparison of the Cardiomyogenic Potency of Human Amniotic Fluid and Bone Marrow Mesenchymal Stem Cells |
title_short | Comparison of the Cardiomyogenic Potency of Human Amniotic Fluid and Bone Marrow Mesenchymal Stem Cells |
title_sort | comparison of the cardiomyogenic potency of human amniotic fluid and bone marrow mesenchymal stem cells |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6881043/ https://www.ncbi.nlm.nih.gov/pubmed/31658508 http://dx.doi.org/10.15283/ijsc18087 |
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