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Circular RNA TTN Acts As a miR-432 Sponge to Facilitate Proliferation and Differentiation of Myoblasts via the IGF2/PI3K/AKT Signaling Pathway

Circular RNAs (circRNAs) are ubiquitous endogenous RNA found in various organisms that can regulate gene expression in eukaryotes. However, little is known about potential roles for circRNAs in muscle development. We analyzed circRNA sequencing data of bovine skeletal muscle tissue and found differe...

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Autores principales: Wang, Xiaogang, Cao, Xiukai, Dong, Dong, Shen, Xuemei, Cheng, Jie, Jiang, Rui, Yang, Zhaoxin, Peng, Shujun, Huang, Yongzhen, Lan, Xianyong, Elnour, Ibrahim Elsaeid, Lei, Chuzhao, Chen, Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Gene & Cell Therapy 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6881651/
https://www.ncbi.nlm.nih.gov/pubmed/31770673
http://dx.doi.org/10.1016/j.omtn.2019.10.019
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author Wang, Xiaogang
Cao, Xiukai
Dong, Dong
Shen, Xuemei
Cheng, Jie
Jiang, Rui
Yang, Zhaoxin
Peng, Shujun
Huang, Yongzhen
Lan, Xianyong
Elnour, Ibrahim Elsaeid
Lei, Chuzhao
Chen, Hong
author_facet Wang, Xiaogang
Cao, Xiukai
Dong, Dong
Shen, Xuemei
Cheng, Jie
Jiang, Rui
Yang, Zhaoxin
Peng, Shujun
Huang, Yongzhen
Lan, Xianyong
Elnour, Ibrahim Elsaeid
Lei, Chuzhao
Chen, Hong
author_sort Wang, Xiaogang
collection PubMed
description Circular RNAs (circRNAs) are ubiquitous endogenous RNA found in various organisms that can regulate gene expression in eukaryotes. However, little is known about potential roles for circRNAs in muscle development. We analyzed circRNA sequencing data of bovine skeletal muscle tissue and found differential expression of circTitin (circTTN) in fetal and adult bovine muscle tissue. We then further studied the role of circTTN in bovine muscle development. Overexpression and inhibition of circTTN together elicited its promoting roles in proliferation and differentiation of bovine primary myoblasts. Mechanistically, circTTN showed interaction with miR-432 by luciferase screening and RNA immunoprecipitation (RIP) assays. Additionally, miR-432 is a regulator of insulin-like growth factor 2 (IGF2), as indicated by luciferase activity, quantitative real-time PCR, and western blotting assays. Increased miR-432 expression inhibited the expression of IGF2, but this effect was remitted by circTTN. Conclusively, our results showed that circTTN promoted proliferation and differentiation of bovine primary myoblasts via competitively combining with miR-432 to activate the IGF2/phosphatidylinositol 3-kinase (PI3K)/AKT signaling pathway.
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spelling pubmed-68816512019-11-29 Circular RNA TTN Acts As a miR-432 Sponge to Facilitate Proliferation and Differentiation of Myoblasts via the IGF2/PI3K/AKT Signaling Pathway Wang, Xiaogang Cao, Xiukai Dong, Dong Shen, Xuemei Cheng, Jie Jiang, Rui Yang, Zhaoxin Peng, Shujun Huang, Yongzhen Lan, Xianyong Elnour, Ibrahim Elsaeid Lei, Chuzhao Chen, Hong Mol Ther Nucleic Acids Article Circular RNAs (circRNAs) are ubiquitous endogenous RNA found in various organisms that can regulate gene expression in eukaryotes. However, little is known about potential roles for circRNAs in muscle development. We analyzed circRNA sequencing data of bovine skeletal muscle tissue and found differential expression of circTitin (circTTN) in fetal and adult bovine muscle tissue. We then further studied the role of circTTN in bovine muscle development. Overexpression and inhibition of circTTN together elicited its promoting roles in proliferation and differentiation of bovine primary myoblasts. Mechanistically, circTTN showed interaction with miR-432 by luciferase screening and RNA immunoprecipitation (RIP) assays. Additionally, miR-432 is a regulator of insulin-like growth factor 2 (IGF2), as indicated by luciferase activity, quantitative real-time PCR, and western blotting assays. Increased miR-432 expression inhibited the expression of IGF2, but this effect was remitted by circTTN. Conclusively, our results showed that circTTN promoted proliferation and differentiation of bovine primary myoblasts via competitively combining with miR-432 to activate the IGF2/phosphatidylinositol 3-kinase (PI3K)/AKT signaling pathway. American Society of Gene & Cell Therapy 2019-10-25 /pmc/articles/PMC6881651/ /pubmed/31770673 http://dx.doi.org/10.1016/j.omtn.2019.10.019 Text en © 2019 The Authors. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Xiaogang
Cao, Xiukai
Dong, Dong
Shen, Xuemei
Cheng, Jie
Jiang, Rui
Yang, Zhaoxin
Peng, Shujun
Huang, Yongzhen
Lan, Xianyong
Elnour, Ibrahim Elsaeid
Lei, Chuzhao
Chen, Hong
Circular RNA TTN Acts As a miR-432 Sponge to Facilitate Proliferation and Differentiation of Myoblasts via the IGF2/PI3K/AKT Signaling Pathway
title Circular RNA TTN Acts As a miR-432 Sponge to Facilitate Proliferation and Differentiation of Myoblasts via the IGF2/PI3K/AKT Signaling Pathway
title_full Circular RNA TTN Acts As a miR-432 Sponge to Facilitate Proliferation and Differentiation of Myoblasts via the IGF2/PI3K/AKT Signaling Pathway
title_fullStr Circular RNA TTN Acts As a miR-432 Sponge to Facilitate Proliferation and Differentiation of Myoblasts via the IGF2/PI3K/AKT Signaling Pathway
title_full_unstemmed Circular RNA TTN Acts As a miR-432 Sponge to Facilitate Proliferation and Differentiation of Myoblasts via the IGF2/PI3K/AKT Signaling Pathway
title_short Circular RNA TTN Acts As a miR-432 Sponge to Facilitate Proliferation and Differentiation of Myoblasts via the IGF2/PI3K/AKT Signaling Pathway
title_sort circular rna ttn acts as a mir-432 sponge to facilitate proliferation and differentiation of myoblasts via the igf2/pi3k/akt signaling pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6881651/
https://www.ncbi.nlm.nih.gov/pubmed/31770673
http://dx.doi.org/10.1016/j.omtn.2019.10.019
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