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Electropermeabilization-based fluorescence in situ hybridization of whole-mount plant parasitic nematode specimens
A fluorescence in situ hybridization (FISH) protocol was developed for nematodes in which nucleic acid probes are introduced within the organism via electroporation. This modification of existing FISH protocols removes numerous chemical wash steps, and thus, reduces protocol time and specimen loss w...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6881680/ https://www.ncbi.nlm.nih.gov/pubmed/31799140 http://dx.doi.org/10.1016/j.mex.2019.11.009 |