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Genome-wide characterization of the rose (Rosa chinensis) WRKY family and role of RcWRKY41 in gray mold resistance

BACKGROUND: The WRKYs are a major family of plant transcription factors that play roles in the responses to biotic and abiotic stresses; however, a comprehensive study of the WRKY family in roses (Rosa sp.) has not previously been performed. RESULTS: In the present study, we performed a genome-wide...

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Autores principales: Liu, Xintong, Li, Dandan, Zhang, Shiya, Xu, Yaling, Zhang, Zhao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6882016/
https://www.ncbi.nlm.nih.gov/pubmed/31775626
http://dx.doi.org/10.1186/s12870-019-2139-6
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author Liu, Xintong
Li, Dandan
Zhang, Shiya
Xu, Yaling
Zhang, Zhao
author_facet Liu, Xintong
Li, Dandan
Zhang, Shiya
Xu, Yaling
Zhang, Zhao
author_sort Liu, Xintong
collection PubMed
description BACKGROUND: The WRKYs are a major family of plant transcription factors that play roles in the responses to biotic and abiotic stresses; however, a comprehensive study of the WRKY family in roses (Rosa sp.) has not previously been performed. RESULTS: In the present study, we performed a genome-wide analysis of the WRKY genes in the rose (Rosa chinensis), including their phylogenetic relationships, gene structure, chromosomal locations, and collinearity. Using a phylogenetic analysis, we divided the 56 RcWRKY genes into three subgroups. The RcWRKYs were unevenly distributed across all seven rose chromosomes, and a study of their collinearity suggested that genome duplication may have played a major role in RcWRKY gene duplication. A Ka/Ks analysis indicated that they mainly underwent purifying selection. Botrytis cinerea infection induced the expression of 19 RcWRKYs, most of which had undergone gene duplication during evolution. These RcWRKYs may regulate rose resistance against B. cinerea. Based on our phylogenetic and expression analyses, RcWRKY41 was identified as a candidate regulatory gene in the response to B. cinerea infection, which was confirmed using virus-induced gene silencing. CONCLUSIONS: This study provides useful information to facilitate the further study of the function of the rose WRKY gene family.
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spelling pubmed-68820162019-12-03 Genome-wide characterization of the rose (Rosa chinensis) WRKY family and role of RcWRKY41 in gray mold resistance Liu, Xintong Li, Dandan Zhang, Shiya Xu, Yaling Zhang, Zhao BMC Plant Biol Research Article BACKGROUND: The WRKYs are a major family of plant transcription factors that play roles in the responses to biotic and abiotic stresses; however, a comprehensive study of the WRKY family in roses (Rosa sp.) has not previously been performed. RESULTS: In the present study, we performed a genome-wide analysis of the WRKY genes in the rose (Rosa chinensis), including their phylogenetic relationships, gene structure, chromosomal locations, and collinearity. Using a phylogenetic analysis, we divided the 56 RcWRKY genes into three subgroups. The RcWRKYs were unevenly distributed across all seven rose chromosomes, and a study of their collinearity suggested that genome duplication may have played a major role in RcWRKY gene duplication. A Ka/Ks analysis indicated that they mainly underwent purifying selection. Botrytis cinerea infection induced the expression of 19 RcWRKYs, most of which had undergone gene duplication during evolution. These RcWRKYs may regulate rose resistance against B. cinerea. Based on our phylogenetic and expression analyses, RcWRKY41 was identified as a candidate regulatory gene in the response to B. cinerea infection, which was confirmed using virus-induced gene silencing. CONCLUSIONS: This study provides useful information to facilitate the further study of the function of the rose WRKY gene family. BioMed Central 2019-11-27 /pmc/articles/PMC6882016/ /pubmed/31775626 http://dx.doi.org/10.1186/s12870-019-2139-6 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Liu, Xintong
Li, Dandan
Zhang, Shiya
Xu, Yaling
Zhang, Zhao
Genome-wide characterization of the rose (Rosa chinensis) WRKY family and role of RcWRKY41 in gray mold resistance
title Genome-wide characterization of the rose (Rosa chinensis) WRKY family and role of RcWRKY41 in gray mold resistance
title_full Genome-wide characterization of the rose (Rosa chinensis) WRKY family and role of RcWRKY41 in gray mold resistance
title_fullStr Genome-wide characterization of the rose (Rosa chinensis) WRKY family and role of RcWRKY41 in gray mold resistance
title_full_unstemmed Genome-wide characterization of the rose (Rosa chinensis) WRKY family and role of RcWRKY41 in gray mold resistance
title_short Genome-wide characterization of the rose (Rosa chinensis) WRKY family and role of RcWRKY41 in gray mold resistance
title_sort genome-wide characterization of the rose (rosa chinensis) wrky family and role of rcwrky41 in gray mold resistance
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6882016/
https://www.ncbi.nlm.nih.gov/pubmed/31775626
http://dx.doi.org/10.1186/s12870-019-2139-6
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