Blocking and re-arrangement of pots in greenhouse experiments: which approach is more effective?

BACKGROUND: Observations measured in field and greenhouse experiments always contain errors. These errors can arise from measurement error, local or positional conditions of the experimental units, or from the randomization of experimental units. In statistical analysis errors can be modelled as independent effects or as spatially correlated effects with an appropriate variance–covariance structure. Using a suitable experimental design, a part of the variance can be captured through blocking of the experimental units. If experimental units (e.g. pots within a greenhouse) are mobile, they can be re-arranged during the experiment. This re-arrangement enables a separation of variation due to time-invariant position effects and variation due to the experimental units. If re-arrangement is successful, the time-invariant positional effect can average out for experimental units moved between different positions during the experiment. While re-arrangement is commonly done in greenhouse experiments, data to quantify its usefulness is limited. RESULTS: A uniformity greenhouse experiment with barley (Hordeum vulgare L.) to compare re-arrangement of pots with a range of designs under fixed-position arrangement showed that both methods can reduce the residual variance and the average standard error of a difference. All designs with fixed-position arrangement, which accounted for the known north–south gradient in the greenhouse, outperformed re-arrangement. An α-design with block size four performed best across seven plant growth traits. CONCLUSION: Blocking with a fixed-position arrangement was more efficient in improving precision of greenhouse experiments than re-arrangement of pots and hence can be recommended for comparable greenhouse experiments.