Cargando…

An Artificial Intelligent Signal Amplification System for in vivo Detection of miRNA

MicroRNAs (miRNA) have been identified as oncogenic drivers and tumor suppressors in every major cancer type. In this work, we design an artificial intelligent signal amplification (AISA) system including double-stranded SQ (S, signal strand; Q, quencher strand) and FP (F, fuel strand; P, protect st...

Descripción completa

Detalles Bibliográficos
Autores principales: Ma, Xibo, Chen, Lei, Yang, Yingcheng, Zhang, Weiqi, Wang, Peixia, Zhang, Kun, Zheng, Bo, Zhu, Lin, Sun, Zheng, Zhang, Shuai, Guo, Yingkun, Liang, Minmin, Wang, Hongyang, Tian, Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6882290/
https://www.ncbi.nlm.nih.gov/pubmed/31824932
http://dx.doi.org/10.3389/fbioe.2019.00330
_version_ 1783474125286670336
author Ma, Xibo
Chen, Lei
Yang, Yingcheng
Zhang, Weiqi
Wang, Peixia
Zhang, Kun
Zheng, Bo
Zhu, Lin
Sun, Zheng
Zhang, Shuai
Guo, Yingkun
Liang, Minmin
Wang, Hongyang
Tian, Jie
author_facet Ma, Xibo
Chen, Lei
Yang, Yingcheng
Zhang, Weiqi
Wang, Peixia
Zhang, Kun
Zheng, Bo
Zhu, Lin
Sun, Zheng
Zhang, Shuai
Guo, Yingkun
Liang, Minmin
Wang, Hongyang
Tian, Jie
author_sort Ma, Xibo
collection PubMed
description MicroRNAs (miRNA) have been identified as oncogenic drivers and tumor suppressors in every major cancer type. In this work, we design an artificial intelligent signal amplification (AISA) system including double-stranded SQ (S, signal strand; Q, quencher strand) and FP (F, fuel strand; P, protect strand) according to thermodynamics principle for sensitive detection of miRNA in vitro and in vivo. In this AISA system for miRNA detection, strand S carries a quenched imaging marker inside the SQ. Target miRNA is constantly replaced by a reaction intermediate and circulatively participates in the reaction, similar to enzyme. Therefore, abundant fluorescent substances from S and SP are dissociated from excessive SQ for in vitro and in vivo visualization. The versatility and feasibility for disease diagnosis using this system were demonstrated by constructing two types of AISA system to detect Hsa-miR-484 and Hsa-miR-100, respectively. The minimum target concentration detected by the system in vitro (10 min after mixing) was 1/10th that of the control group. The precancerous lesions of liver cancer were diagnosed, and the detection accuracy were larger than 94% both in terms of location and concentration. The ability to establish this design framework for AISA system with high specificity provides a new way to monitor tumor progression and to assess therapeutic responses.
format Online
Article
Text
id pubmed-6882290
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-68822902019-12-10 An Artificial Intelligent Signal Amplification System for in vivo Detection of miRNA Ma, Xibo Chen, Lei Yang, Yingcheng Zhang, Weiqi Wang, Peixia Zhang, Kun Zheng, Bo Zhu, Lin Sun, Zheng Zhang, Shuai Guo, Yingkun Liang, Minmin Wang, Hongyang Tian, Jie Front Bioeng Biotechnol Bioengineering and Biotechnology MicroRNAs (miRNA) have been identified as oncogenic drivers and tumor suppressors in every major cancer type. In this work, we design an artificial intelligent signal amplification (AISA) system including double-stranded SQ (S, signal strand; Q, quencher strand) and FP (F, fuel strand; P, protect strand) according to thermodynamics principle for sensitive detection of miRNA in vitro and in vivo. In this AISA system for miRNA detection, strand S carries a quenched imaging marker inside the SQ. Target miRNA is constantly replaced by a reaction intermediate and circulatively participates in the reaction, similar to enzyme. Therefore, abundant fluorescent substances from S and SP are dissociated from excessive SQ for in vitro and in vivo visualization. The versatility and feasibility for disease diagnosis using this system were demonstrated by constructing two types of AISA system to detect Hsa-miR-484 and Hsa-miR-100, respectively. The minimum target concentration detected by the system in vitro (10 min after mixing) was 1/10th that of the control group. The precancerous lesions of liver cancer were diagnosed, and the detection accuracy were larger than 94% both in terms of location and concentration. The ability to establish this design framework for AISA system with high specificity provides a new way to monitor tumor progression and to assess therapeutic responses. Frontiers Media S.A. 2019-11-21 /pmc/articles/PMC6882290/ /pubmed/31824932 http://dx.doi.org/10.3389/fbioe.2019.00330 Text en Copyright © 2019 Ma, Chen, Yang, Zhang, Wang, Zhang, Zheng, Zhu, Sun, Zhang, Guo, Liang, Wang and Tian. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Ma, Xibo
Chen, Lei
Yang, Yingcheng
Zhang, Weiqi
Wang, Peixia
Zhang, Kun
Zheng, Bo
Zhu, Lin
Sun, Zheng
Zhang, Shuai
Guo, Yingkun
Liang, Minmin
Wang, Hongyang
Tian, Jie
An Artificial Intelligent Signal Amplification System for in vivo Detection of miRNA
title An Artificial Intelligent Signal Amplification System for in vivo Detection of miRNA
title_full An Artificial Intelligent Signal Amplification System for in vivo Detection of miRNA
title_fullStr An Artificial Intelligent Signal Amplification System for in vivo Detection of miRNA
title_full_unstemmed An Artificial Intelligent Signal Amplification System for in vivo Detection of miRNA
title_short An Artificial Intelligent Signal Amplification System for in vivo Detection of miRNA
title_sort artificial intelligent signal amplification system for in vivo detection of mirna
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6882290/
https://www.ncbi.nlm.nih.gov/pubmed/31824932
http://dx.doi.org/10.3389/fbioe.2019.00330
work_keys_str_mv AT maxibo anartificialintelligentsignalamplificationsystemforinvivodetectionofmirna
AT chenlei anartificialintelligentsignalamplificationsystemforinvivodetectionofmirna
AT yangyingcheng anartificialintelligentsignalamplificationsystemforinvivodetectionofmirna
AT zhangweiqi anartificialintelligentsignalamplificationsystemforinvivodetectionofmirna
AT wangpeixia anartificialintelligentsignalamplificationsystemforinvivodetectionofmirna
AT zhangkun anartificialintelligentsignalamplificationsystemforinvivodetectionofmirna
AT zhengbo anartificialintelligentsignalamplificationsystemforinvivodetectionofmirna
AT zhulin anartificialintelligentsignalamplificationsystemforinvivodetectionofmirna
AT sunzheng anartificialintelligentsignalamplificationsystemforinvivodetectionofmirna
AT zhangshuai anartificialintelligentsignalamplificationsystemforinvivodetectionofmirna
AT guoyingkun anartificialintelligentsignalamplificationsystemforinvivodetectionofmirna
AT liangminmin anartificialintelligentsignalamplificationsystemforinvivodetectionofmirna
AT wanghongyang anartificialintelligentsignalamplificationsystemforinvivodetectionofmirna
AT tianjie anartificialintelligentsignalamplificationsystemforinvivodetectionofmirna
AT maxibo artificialintelligentsignalamplificationsystemforinvivodetectionofmirna
AT chenlei artificialintelligentsignalamplificationsystemforinvivodetectionofmirna
AT yangyingcheng artificialintelligentsignalamplificationsystemforinvivodetectionofmirna
AT zhangweiqi artificialintelligentsignalamplificationsystemforinvivodetectionofmirna
AT wangpeixia artificialintelligentsignalamplificationsystemforinvivodetectionofmirna
AT zhangkun artificialintelligentsignalamplificationsystemforinvivodetectionofmirna
AT zhengbo artificialintelligentsignalamplificationsystemforinvivodetectionofmirna
AT zhulin artificialintelligentsignalamplificationsystemforinvivodetectionofmirna
AT sunzheng artificialintelligentsignalamplificationsystemforinvivodetectionofmirna
AT zhangshuai artificialintelligentsignalamplificationsystemforinvivodetectionofmirna
AT guoyingkun artificialintelligentsignalamplificationsystemforinvivodetectionofmirna
AT liangminmin artificialintelligentsignalamplificationsystemforinvivodetectionofmirna
AT wanghongyang artificialintelligentsignalamplificationsystemforinvivodetectionofmirna
AT tianjie artificialintelligentsignalamplificationsystemforinvivodetectionofmirna