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Population dynamics and antigenic drift of Bordetella pertussis following whole cell vaccine replacement, Barcelona, Spain, 1986–2015

Among the factors associated with the resurgence of whooping cough, special emphasis has been given to pathogen adaptation after the introduction of the acellular vaccine (ACV). To assess the impact of the vaccine transition strategy from whole-cell vaccine (WCV) to ACV on population dynamics of Bor...

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Autores principales: Mir-Cros, Alba, Moreno-Mingorance, Albert, Martín-Gómez, M. Teresa, Codina, Gema, Cornejo-Sánchez, Thais, Rajadell, Mireia, Van Esso, Diego, Rodrigo, Carlos, Campins, Magda, Jané, Mireia, Pumarola, Tomàs, Fàbrega, Anna, González-López, Juan José
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6882445/
https://www.ncbi.nlm.nih.gov/pubmed/31769735
http://dx.doi.org/10.1080/22221751.2019.1694395
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author Mir-Cros, Alba
Moreno-Mingorance, Albert
Martín-Gómez, M. Teresa
Codina, Gema
Cornejo-Sánchez, Thais
Rajadell, Mireia
Van Esso, Diego
Rodrigo, Carlos
Campins, Magda
Jané, Mireia
Pumarola, Tomàs
Fàbrega, Anna
González-López, Juan José
author_facet Mir-Cros, Alba
Moreno-Mingorance, Albert
Martín-Gómez, M. Teresa
Codina, Gema
Cornejo-Sánchez, Thais
Rajadell, Mireia
Van Esso, Diego
Rodrigo, Carlos
Campins, Magda
Jané, Mireia
Pumarola, Tomàs
Fàbrega, Anna
González-López, Juan José
author_sort Mir-Cros, Alba
collection PubMed
description Among the factors associated with the resurgence of whooping cough, special emphasis has been given to pathogen adaptation after the introduction of the acellular vaccine (ACV). To assess the impact of the vaccine transition strategy from whole-cell vaccine (WCV) to ACV on population dynamics of Bordetella pertussis in Barcelona (Spain), we studied 339 isolates collected from 1986 to 2015 by PFGE and multi-locus variable-number tandem repeat analysis (MLVA). Additionally, allelic variants for the pertussis toxin and its promoter, pertactin, type 3 fimbriae and fimbrial serotyping were assessed to determine its antigenic drift. A shift was observed in the B. pertussis population as well as in its antigenic profile concurrently with the introduction of ACV in Barcelona. Four out of the five most prevalent PFGE profiles were replaced by new profiles following the ACV introduction. MLVA type 27 was the dominant genotype, and its frequency increased from 25% to 79.3% after WCV replacement. Antigen typing demonstrated the emergence of prn2, ptxP3, fim3-2 and a shift from the fimbriae 3 to the fimbriae 2 serotypes after the ACV introduction. Our findings support the presence of population and antigenic dynamic changes in B. pertussis likely driven by the introduction of ACV.
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spelling pubmed-68824452019-12-13 Population dynamics and antigenic drift of Bordetella pertussis following whole cell vaccine replacement, Barcelona, Spain, 1986–2015 Mir-Cros, Alba Moreno-Mingorance, Albert Martín-Gómez, M. Teresa Codina, Gema Cornejo-Sánchez, Thais Rajadell, Mireia Van Esso, Diego Rodrigo, Carlos Campins, Magda Jané, Mireia Pumarola, Tomàs Fàbrega, Anna González-López, Juan José Emerg Microbes Infect Original Articles Among the factors associated with the resurgence of whooping cough, special emphasis has been given to pathogen adaptation after the introduction of the acellular vaccine (ACV). To assess the impact of the vaccine transition strategy from whole-cell vaccine (WCV) to ACV on population dynamics of Bordetella pertussis in Barcelona (Spain), we studied 339 isolates collected from 1986 to 2015 by PFGE and multi-locus variable-number tandem repeat analysis (MLVA). Additionally, allelic variants for the pertussis toxin and its promoter, pertactin, type 3 fimbriae and fimbrial serotyping were assessed to determine its antigenic drift. A shift was observed in the B. pertussis population as well as in its antigenic profile concurrently with the introduction of ACV in Barcelona. Four out of the five most prevalent PFGE profiles were replaced by new profiles following the ACV introduction. MLVA type 27 was the dominant genotype, and its frequency increased from 25% to 79.3% after WCV replacement. Antigen typing demonstrated the emergence of prn2, ptxP3, fim3-2 and a shift from the fimbriae 3 to the fimbriae 2 serotypes after the ACV introduction. Our findings support the presence of population and antigenic dynamic changes in B. pertussis likely driven by the introduction of ACV. Taylor & Francis 2019-11-26 /pmc/articles/PMC6882445/ /pubmed/31769735 http://dx.doi.org/10.1080/22221751.2019.1694395 Text en © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Mir-Cros, Alba
Moreno-Mingorance, Albert
Martín-Gómez, M. Teresa
Codina, Gema
Cornejo-Sánchez, Thais
Rajadell, Mireia
Van Esso, Diego
Rodrigo, Carlos
Campins, Magda
Jané, Mireia
Pumarola, Tomàs
Fàbrega, Anna
González-López, Juan José
Population dynamics and antigenic drift of Bordetella pertussis following whole cell vaccine replacement, Barcelona, Spain, 1986–2015
title Population dynamics and antigenic drift of Bordetella pertussis following whole cell vaccine replacement, Barcelona, Spain, 1986–2015
title_full Population dynamics and antigenic drift of Bordetella pertussis following whole cell vaccine replacement, Barcelona, Spain, 1986–2015
title_fullStr Population dynamics and antigenic drift of Bordetella pertussis following whole cell vaccine replacement, Barcelona, Spain, 1986–2015
title_full_unstemmed Population dynamics and antigenic drift of Bordetella pertussis following whole cell vaccine replacement, Barcelona, Spain, 1986–2015
title_short Population dynamics and antigenic drift of Bordetella pertussis following whole cell vaccine replacement, Barcelona, Spain, 1986–2015
title_sort population dynamics and antigenic drift of bordetella pertussis following whole cell vaccine replacement, barcelona, spain, 1986–2015
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6882445/
https://www.ncbi.nlm.nih.gov/pubmed/31769735
http://dx.doi.org/10.1080/22221751.2019.1694395
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