Cargando…

Diurnal variation of inflammatory plasma proteins involved in pain

INTRODUCTION: Proteomics is a powerful approach for biochemical research because it directly studies the main functional components of biochemical systems. The understanding of the normal fluctuations of the proteome in health is essential to identify pain-specific biomarkers. OBJECTIVE: To investig...

Descripción completa

Detalles Bibliográficos
Autores principales: Jasim, Hajer, Carlsson, Anders, Gerdle, Björn, Ernberg, Malin, Ghafouri, Bijar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6882578/
https://www.ncbi.nlm.nih.gov/pubmed/31875183
http://dx.doi.org/10.1097/PR9.0000000000000776
_version_ 1783474191284043776
author Jasim, Hajer
Carlsson, Anders
Gerdle, Björn
Ernberg, Malin
Ghafouri, Bijar
author_facet Jasim, Hajer
Carlsson, Anders
Gerdle, Björn
Ernberg, Malin
Ghafouri, Bijar
author_sort Jasim, Hajer
collection PubMed
description INTRODUCTION: Proteomics is a powerful approach for biochemical research because it directly studies the main functional components of biochemical systems. The understanding of the normal fluctuations of the proteome in health is essential to identify pain-specific biomarkers. OBJECTIVE: To investigate fluctuations of the plasma proteome in healthy pain-free individuals. METHODS: Blood samples were structurally collected in the early morning and evening from 10 clinically healthy individuals (26.3 ± 3.3 years). High abundant proteins were removed from plasma, and proteins were then analysed by nanoliquid chromatography combined with mass spectrometry. In addition, an assay of 71 cytokines/chemokines/growth factors was analysed. RESULTS: Multivariate statistical analysis displayed that there were up to 64 proteins whose expression levels were significantly altered between the plasma samples collected during the morning and evening; no changes existed for the assay. The levels of 34 proteins were increased and 30 proteins were decreased during the evening compared with the morning sample. The increased proteins were involved in the biological processes such as protein activation cascade, complement activation, and stress response. The decreased proteins were involved in regulation of endopeptidase activity, inflammatory response, and protein metabolic processes. CONCLUSION: The circadian variations in the plasma proteome stress the need to collect blood samples of both patients and controls at a fixed time of the day. The results in this study might be useful for better understanding of the complexity of individual variation in the human plasma proteome over time and provide a baseline for improved pain biomarker discovery.
format Online
Article
Text
id pubmed-6882578
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Wolters Kluwer
record_format MEDLINE/PubMed
spelling pubmed-68825782019-12-24 Diurnal variation of inflammatory plasma proteins involved in pain Jasim, Hajer Carlsson, Anders Gerdle, Björn Ernberg, Malin Ghafouri, Bijar Pain Rep General Section INTRODUCTION: Proteomics is a powerful approach for biochemical research because it directly studies the main functional components of biochemical systems. The understanding of the normal fluctuations of the proteome in health is essential to identify pain-specific biomarkers. OBJECTIVE: To investigate fluctuations of the plasma proteome in healthy pain-free individuals. METHODS: Blood samples were structurally collected in the early morning and evening from 10 clinically healthy individuals (26.3 ± 3.3 years). High abundant proteins were removed from plasma, and proteins were then analysed by nanoliquid chromatography combined with mass spectrometry. In addition, an assay of 71 cytokines/chemokines/growth factors was analysed. RESULTS: Multivariate statistical analysis displayed that there were up to 64 proteins whose expression levels were significantly altered between the plasma samples collected during the morning and evening; no changes existed for the assay. The levels of 34 proteins were increased and 30 proteins were decreased during the evening compared with the morning sample. The increased proteins were involved in the biological processes such as protein activation cascade, complement activation, and stress response. The decreased proteins were involved in regulation of endopeptidase activity, inflammatory response, and protein metabolic processes. CONCLUSION: The circadian variations in the plasma proteome stress the need to collect blood samples of both patients and controls at a fixed time of the day. The results in this study might be useful for better understanding of the complexity of individual variation in the human plasma proteome over time and provide a baseline for improved pain biomarker discovery. Wolters Kluwer 2019-08-13 /pmc/articles/PMC6882578/ /pubmed/31875183 http://dx.doi.org/10.1097/PR9.0000000000000776 Text en Copyright © 2019 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of The International Association for the Study of Pain. This is an open access article distributed under the Creative Commons Attribution License 4.0 (CCBY) (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle General Section
Jasim, Hajer
Carlsson, Anders
Gerdle, Björn
Ernberg, Malin
Ghafouri, Bijar
Diurnal variation of inflammatory plasma proteins involved in pain
title Diurnal variation of inflammatory plasma proteins involved in pain
title_full Diurnal variation of inflammatory plasma proteins involved in pain
title_fullStr Diurnal variation of inflammatory plasma proteins involved in pain
title_full_unstemmed Diurnal variation of inflammatory plasma proteins involved in pain
title_short Diurnal variation of inflammatory plasma proteins involved in pain
title_sort diurnal variation of inflammatory plasma proteins involved in pain
topic General Section
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6882578/
https://www.ncbi.nlm.nih.gov/pubmed/31875183
http://dx.doi.org/10.1097/PR9.0000000000000776
work_keys_str_mv AT jasimhajer diurnalvariationofinflammatoryplasmaproteinsinvolvedinpain
AT carlssonanders diurnalvariationofinflammatoryplasmaproteinsinvolvedinpain
AT gerdlebjorn diurnalvariationofinflammatoryplasmaproteinsinvolvedinpain
AT ernbergmalin diurnalvariationofinflammatoryplasmaproteinsinvolvedinpain
AT ghafouribijar diurnalvariationofinflammatoryplasmaproteinsinvolvedinpain