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Expression of GluA2-containing calcium-impermeable AMPA receptors on dopaminergic amacrine cells in the mouse retina
PURPOSE: The neuromodulator dopamine plays an important role in light adaptation for the visual system. Light can stimulate dopamine release from dopaminergic amacrine cells (DACs) by activating three classes of photosensitive retinal cells: rods, cones, and melanopsin-expressing intrinsically photo...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Vision
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6882663/ https://www.ncbi.nlm.nih.gov/pubmed/31819340 |
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author | Liu, Lei-Lei Alessio, Elizabeth J. Spix, Nathan J. Zhang, Dao-Qi |
author_facet | Liu, Lei-Lei Alessio, Elizabeth J. Spix, Nathan J. Zhang, Dao-Qi |
author_sort | Liu, Lei-Lei |
collection | PubMed |
description | PURPOSE: The neuromodulator dopamine plays an important role in light adaptation for the visual system. Light can stimulate dopamine release from dopaminergic amacrine cells (DACs) by activating three classes of photosensitive retinal cells: rods, cones, and melanopsin-expressing intrinsically photosensitive retinal ganglion cells (ipRGCs). However, the synaptic mechanisms by which these photoreceptors excite DACs remain poorly understood. Our previous work demonstrated that α-amino-3-hydroxyl-5-methyl-4-isoxazole-propionate (AMPA) receptors contribute to light regulation of DAC activity. AMPA receptors are classified into Ca(2+)-permeable and Ca(2+)-impermeable subtypes. We sought to identify which subtype of AMPA receptors is involved in light regulation of DAC activity. METHODS: AMPA receptor-mediated light responses and miniature excitatory postsynaptic currents were recorded from genetically labeled DACs in mouse retinas with the whole-cell voltage-clamp mode. Immunostaining with antibodies against tyrosine hydroxylase, GluA2 (GluR2), and PSD-95 was performed in vertical retinal slices. RESULTS: The biophysical and pharmacological data showed that only Ca(2+)-impermeable AMPA receptors contribute to DAC light responses driven by ipRGCs or cones (via depolarizing bipolar cells). We further found that the same subtype of AMPA receptors mediates miniature excitatory postsynaptic currents of DACs. These findings are supported by the immunohistochemical results demonstrating that DACs express the PSD-95 with GluA2, a subunit that is essential for determining the impermeability of AMPA receptors to calcium. CONCLUSIONS: The results indicated that GluA2-containing Ca(2+)-impermeable AMPA receptors contribute to signal transmission from photosensitive retinal cells to DACs. |
format | Online Article Text |
id | pubmed-6882663 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Molecular Vision |
record_format | MEDLINE/PubMed |
spelling | pubmed-68826632019-12-09 Expression of GluA2-containing calcium-impermeable AMPA receptors on dopaminergic amacrine cells in the mouse retina Liu, Lei-Lei Alessio, Elizabeth J. Spix, Nathan J. Zhang, Dao-Qi Mol Vis Research Article PURPOSE: The neuromodulator dopamine plays an important role in light adaptation for the visual system. Light can stimulate dopamine release from dopaminergic amacrine cells (DACs) by activating three classes of photosensitive retinal cells: rods, cones, and melanopsin-expressing intrinsically photosensitive retinal ganglion cells (ipRGCs). However, the synaptic mechanisms by which these photoreceptors excite DACs remain poorly understood. Our previous work demonstrated that α-amino-3-hydroxyl-5-methyl-4-isoxazole-propionate (AMPA) receptors contribute to light regulation of DAC activity. AMPA receptors are classified into Ca(2+)-permeable and Ca(2+)-impermeable subtypes. We sought to identify which subtype of AMPA receptors is involved in light regulation of DAC activity. METHODS: AMPA receptor-mediated light responses and miniature excitatory postsynaptic currents were recorded from genetically labeled DACs in mouse retinas with the whole-cell voltage-clamp mode. Immunostaining with antibodies against tyrosine hydroxylase, GluA2 (GluR2), and PSD-95 was performed in vertical retinal slices. RESULTS: The biophysical and pharmacological data showed that only Ca(2+)-impermeable AMPA receptors contribute to DAC light responses driven by ipRGCs or cones (via depolarizing bipolar cells). We further found that the same subtype of AMPA receptors mediates miniature excitatory postsynaptic currents of DACs. These findings are supported by the immunohistochemical results demonstrating that DACs express the PSD-95 with GluA2, a subunit that is essential for determining the impermeability of AMPA receptors to calcium. CONCLUSIONS: The results indicated that GluA2-containing Ca(2+)-impermeable AMPA receptors contribute to signal transmission from photosensitive retinal cells to DACs. Molecular Vision 2019-11-19 /pmc/articles/PMC6882663/ /pubmed/31819340 Text en Copyright © 2019 Molecular Vision. http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited, used for non-commercial purposes, and is not altered or transformed. |
spellingShingle | Research Article Liu, Lei-Lei Alessio, Elizabeth J. Spix, Nathan J. Zhang, Dao-Qi Expression of GluA2-containing calcium-impermeable AMPA receptors on dopaminergic amacrine cells in the mouse retina |
title | Expression of GluA2-containing calcium-impermeable AMPA receptors on dopaminergic amacrine cells in the mouse retina |
title_full | Expression of GluA2-containing calcium-impermeable AMPA receptors on dopaminergic amacrine cells in the mouse retina |
title_fullStr | Expression of GluA2-containing calcium-impermeable AMPA receptors on dopaminergic amacrine cells in the mouse retina |
title_full_unstemmed | Expression of GluA2-containing calcium-impermeable AMPA receptors on dopaminergic amacrine cells in the mouse retina |
title_short | Expression of GluA2-containing calcium-impermeable AMPA receptors on dopaminergic amacrine cells in the mouse retina |
title_sort | expression of glua2-containing calcium-impermeable ampa receptors on dopaminergic amacrine cells in the mouse retina |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6882663/ https://www.ncbi.nlm.nih.gov/pubmed/31819340 |
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