Cargando…

Expression of GluA2-containing calcium-impermeable AMPA receptors on dopaminergic amacrine cells in the mouse retina

PURPOSE: The neuromodulator dopamine plays an important role in light adaptation for the visual system. Light can stimulate dopamine release from dopaminergic amacrine cells (DACs) by activating three classes of photosensitive retinal cells: rods, cones, and melanopsin-expressing intrinsically photo...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Lei-Lei, Alessio, Elizabeth J., Spix, Nathan J., Zhang, Dao-Qi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Vision 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6882663/
https://www.ncbi.nlm.nih.gov/pubmed/31819340
_version_ 1783474211553017856
author Liu, Lei-Lei
Alessio, Elizabeth J.
Spix, Nathan J.
Zhang, Dao-Qi
author_facet Liu, Lei-Lei
Alessio, Elizabeth J.
Spix, Nathan J.
Zhang, Dao-Qi
author_sort Liu, Lei-Lei
collection PubMed
description PURPOSE: The neuromodulator dopamine plays an important role in light adaptation for the visual system. Light can stimulate dopamine release from dopaminergic amacrine cells (DACs) by activating three classes of photosensitive retinal cells: rods, cones, and melanopsin-expressing intrinsically photosensitive retinal ganglion cells (ipRGCs). However, the synaptic mechanisms by which these photoreceptors excite DACs remain poorly understood. Our previous work demonstrated that α-amino-3-hydroxyl-5-methyl-4-isoxazole-propionate (AMPA) receptors contribute to light regulation of DAC activity. AMPA receptors are classified into Ca(2+)-permeable and Ca(2+)-impermeable subtypes. We sought to identify which subtype of AMPA receptors is involved in light regulation of DAC activity. METHODS: AMPA receptor-mediated light responses and miniature excitatory postsynaptic currents were recorded from genetically labeled DACs in mouse retinas with the whole-cell voltage-clamp mode. Immunostaining with antibodies against tyrosine hydroxylase, GluA2 (GluR2), and PSD-95 was performed in vertical retinal slices. RESULTS: The biophysical and pharmacological data showed that only Ca(2+)-impermeable AMPA receptors contribute to DAC light responses driven by ipRGCs or cones (via depolarizing bipolar cells). We further found that the same subtype of AMPA receptors mediates miniature excitatory postsynaptic currents of DACs. These findings are supported by the immunohistochemical results demonstrating that DACs express the PSD-95 with GluA2, a subunit that is essential for determining the impermeability of AMPA receptors to calcium. CONCLUSIONS: The results indicated that GluA2-containing Ca(2+)-impermeable AMPA receptors contribute to signal transmission from photosensitive retinal cells to DACs.
format Online
Article
Text
id pubmed-6882663
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Molecular Vision
record_format MEDLINE/PubMed
spelling pubmed-68826632019-12-09 Expression of GluA2-containing calcium-impermeable AMPA receptors on dopaminergic amacrine cells in the mouse retina Liu, Lei-Lei Alessio, Elizabeth J. Spix, Nathan J. Zhang, Dao-Qi Mol Vis Research Article PURPOSE: The neuromodulator dopamine plays an important role in light adaptation for the visual system. Light can stimulate dopamine release from dopaminergic amacrine cells (DACs) by activating three classes of photosensitive retinal cells: rods, cones, and melanopsin-expressing intrinsically photosensitive retinal ganglion cells (ipRGCs). However, the synaptic mechanisms by which these photoreceptors excite DACs remain poorly understood. Our previous work demonstrated that α-amino-3-hydroxyl-5-methyl-4-isoxazole-propionate (AMPA) receptors contribute to light regulation of DAC activity. AMPA receptors are classified into Ca(2+)-permeable and Ca(2+)-impermeable subtypes. We sought to identify which subtype of AMPA receptors is involved in light regulation of DAC activity. METHODS: AMPA receptor-mediated light responses and miniature excitatory postsynaptic currents were recorded from genetically labeled DACs in mouse retinas with the whole-cell voltage-clamp mode. Immunostaining with antibodies against tyrosine hydroxylase, GluA2 (GluR2), and PSD-95 was performed in vertical retinal slices. RESULTS: The biophysical and pharmacological data showed that only Ca(2+)-impermeable AMPA receptors contribute to DAC light responses driven by ipRGCs or cones (via depolarizing bipolar cells). We further found that the same subtype of AMPA receptors mediates miniature excitatory postsynaptic currents of DACs. These findings are supported by the immunohistochemical results demonstrating that DACs express the PSD-95 with GluA2, a subunit that is essential for determining the impermeability of AMPA receptors to calcium. CONCLUSIONS: The results indicated that GluA2-containing Ca(2+)-impermeable AMPA receptors contribute to signal transmission from photosensitive retinal cells to DACs. Molecular Vision 2019-11-19 /pmc/articles/PMC6882663/ /pubmed/31819340 Text en Copyright © 2019 Molecular Vision. http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited, used for non-commercial purposes, and is not altered or transformed.
spellingShingle Research Article
Liu, Lei-Lei
Alessio, Elizabeth J.
Spix, Nathan J.
Zhang, Dao-Qi
Expression of GluA2-containing calcium-impermeable AMPA receptors on dopaminergic amacrine cells in the mouse retina
title Expression of GluA2-containing calcium-impermeable AMPA receptors on dopaminergic amacrine cells in the mouse retina
title_full Expression of GluA2-containing calcium-impermeable AMPA receptors on dopaminergic amacrine cells in the mouse retina
title_fullStr Expression of GluA2-containing calcium-impermeable AMPA receptors on dopaminergic amacrine cells in the mouse retina
title_full_unstemmed Expression of GluA2-containing calcium-impermeable AMPA receptors on dopaminergic amacrine cells in the mouse retina
title_short Expression of GluA2-containing calcium-impermeable AMPA receptors on dopaminergic amacrine cells in the mouse retina
title_sort expression of glua2-containing calcium-impermeable ampa receptors on dopaminergic amacrine cells in the mouse retina
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6882663/
https://www.ncbi.nlm.nih.gov/pubmed/31819340
work_keys_str_mv AT liuleilei expressionofglua2containingcalciumimpermeableampareceptorsondopaminergicamacrinecellsinthemouseretina
AT alessioelizabethj expressionofglua2containingcalciumimpermeableampareceptorsondopaminergicamacrinecellsinthemouseretina
AT spixnathanj expressionofglua2containingcalciumimpermeableampareceptorsondopaminergicamacrinecellsinthemouseretina
AT zhangdaoqi expressionofglua2containingcalciumimpermeableampareceptorsondopaminergicamacrinecellsinthemouseretina