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Transcriptional control of central carbon metabolic flux in Bifidobacteria by two functionally similar, yet distinct LacI-type regulators

Bifidobacteria resident in the gastrointestinal tract (GIT) are subject to constantly changing environmental conditions, which require rapid adjustments in gene expression. Here, we show that two predicted LacI-type transcription factors (TFs), designated AraQ and MalR1, are involved in regulating t...

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Autores principales: Lanigan, Noreen, Kelly, Emer, Arzamasov, Aleksandr A., Stanton, Catherine, Rodionov, Dmitry A., van Sinderen, Douwe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6882875/
https://www.ncbi.nlm.nih.gov/pubmed/31780796
http://dx.doi.org/10.1038/s41598-019-54229-4
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author Lanigan, Noreen
Kelly, Emer
Arzamasov, Aleksandr A.
Stanton, Catherine
Rodionov, Dmitry A.
van Sinderen, Douwe
author_facet Lanigan, Noreen
Kelly, Emer
Arzamasov, Aleksandr A.
Stanton, Catherine
Rodionov, Dmitry A.
van Sinderen, Douwe
author_sort Lanigan, Noreen
collection PubMed
description Bifidobacteria resident in the gastrointestinal tract (GIT) are subject to constantly changing environmental conditions, which require rapid adjustments in gene expression. Here, we show that two predicted LacI-type transcription factors (TFs), designated AraQ and MalR1, are involved in regulating the central, carbohydrate-associated metabolic pathway (the so-called phosphoketolase pathway or bifid shunt) of the gut commensal Bifidobacterium breve UCC2003. These TFs appear to not only control transcription of genes involved in the bifid shunt and each other, but also seem to commonly and directly affect transcription of other TF-encoding genes, as well as genes related to uptake and metabolism of various carbohydrates. This complex and interactive network of AraQ/MalR1-mediated gene regulation provides previously unknown insights into the governance of carbon metabolism in bifidobacteria.
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spelling pubmed-68828752019-12-06 Transcriptional control of central carbon metabolic flux in Bifidobacteria by two functionally similar, yet distinct LacI-type regulators Lanigan, Noreen Kelly, Emer Arzamasov, Aleksandr A. Stanton, Catherine Rodionov, Dmitry A. van Sinderen, Douwe Sci Rep Article Bifidobacteria resident in the gastrointestinal tract (GIT) are subject to constantly changing environmental conditions, which require rapid adjustments in gene expression. Here, we show that two predicted LacI-type transcription factors (TFs), designated AraQ and MalR1, are involved in regulating the central, carbohydrate-associated metabolic pathway (the so-called phosphoketolase pathway or bifid shunt) of the gut commensal Bifidobacterium breve UCC2003. These TFs appear to not only control transcription of genes involved in the bifid shunt and each other, but also seem to commonly and directly affect transcription of other TF-encoding genes, as well as genes related to uptake and metabolism of various carbohydrates. This complex and interactive network of AraQ/MalR1-mediated gene regulation provides previously unknown insights into the governance of carbon metabolism in bifidobacteria. Nature Publishing Group UK 2019-11-28 /pmc/articles/PMC6882875/ /pubmed/31780796 http://dx.doi.org/10.1038/s41598-019-54229-4 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Lanigan, Noreen
Kelly, Emer
Arzamasov, Aleksandr A.
Stanton, Catherine
Rodionov, Dmitry A.
van Sinderen, Douwe
Transcriptional control of central carbon metabolic flux in Bifidobacteria by two functionally similar, yet distinct LacI-type regulators
title Transcriptional control of central carbon metabolic flux in Bifidobacteria by two functionally similar, yet distinct LacI-type regulators
title_full Transcriptional control of central carbon metabolic flux in Bifidobacteria by two functionally similar, yet distinct LacI-type regulators
title_fullStr Transcriptional control of central carbon metabolic flux in Bifidobacteria by two functionally similar, yet distinct LacI-type regulators
title_full_unstemmed Transcriptional control of central carbon metabolic flux in Bifidobacteria by two functionally similar, yet distinct LacI-type regulators
title_short Transcriptional control of central carbon metabolic flux in Bifidobacteria by two functionally similar, yet distinct LacI-type regulators
title_sort transcriptional control of central carbon metabolic flux in bifidobacteria by two functionally similar, yet distinct laci-type regulators
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6882875/
https://www.ncbi.nlm.nih.gov/pubmed/31780796
http://dx.doi.org/10.1038/s41598-019-54229-4
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