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Arsenic metabolism in technical biogas plants: possible consequences for resident microbiota and downstream units

The metal(loid) and in particular the Arsenic (As) burden of thirteen agricultural biogas plants and two sewage sludge digesters were investigated together with the corresponding microbial consortia. The latter were characterized by ARISA (automated ribosomal intergenetic spacer analysis) and next g...

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Autores principales: Weithmann, Nicolas, Mlinar, Stanislava, Hilbrig, Frank, Bachmaf, Samer, Arndt, Julia, Planer-Friedrich, Britta, Weig, Alfons R., Freitag, Ruth
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6882981/
https://www.ncbi.nlm.nih.gov/pubmed/31781978
http://dx.doi.org/10.1186/s13568-019-0902-6
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author Weithmann, Nicolas
Mlinar, Stanislava
Hilbrig, Frank
Bachmaf, Samer
Arndt, Julia
Planer-Friedrich, Britta
Weig, Alfons R.
Freitag, Ruth
author_facet Weithmann, Nicolas
Mlinar, Stanislava
Hilbrig, Frank
Bachmaf, Samer
Arndt, Julia
Planer-Friedrich, Britta
Weig, Alfons R.
Freitag, Ruth
author_sort Weithmann, Nicolas
collection PubMed
description The metal(loid) and in particular the Arsenic (As) burden of thirteen agricultural biogas plants and two sewage sludge digesters were investigated together with the corresponding microbial consortia. The latter were characterized by ARISA (automated ribosomal intergenetic spacer analysis) and next generation sequencing. The consortia were found to cluster according to digester type rather than substrate or metal(loid) composition. For selected plants, individual As species in the liquid and gaseous phases were quantified, showing that the microorganisms actively metabolize and thereby remove the As from their environment via the formation of (methylated) volatile species. The As metabolites showed some dependency on the microbial consortia, while there was no statistical correlation with the substrate mix. Finally, slurry from one agricultural biogas plant and one sewage sludge digester was transferred into laboratory scale reactors (“satellite reactors”) and the response to a defined addition of As (30 and 60 µM sodium arsenite) was studied. The results corroborate the hypothesis of a rapid conversion of dissolved As species into volatile ones. Methanogenesis was reduced during that time, while there was no discernable toxic effect on the microbial population. However, the utilization of the produced biogas as replacement for natural gas, e.g. as fuel, may be problematic, as catalysts and machinery are known to suffer from prolonged exposure even to low As concentrations.
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spelling pubmed-68829812019-12-12 Arsenic metabolism in technical biogas plants: possible consequences for resident microbiota and downstream units Weithmann, Nicolas Mlinar, Stanislava Hilbrig, Frank Bachmaf, Samer Arndt, Julia Planer-Friedrich, Britta Weig, Alfons R. Freitag, Ruth AMB Express Original Article The metal(loid) and in particular the Arsenic (As) burden of thirteen agricultural biogas plants and two sewage sludge digesters were investigated together with the corresponding microbial consortia. The latter were characterized by ARISA (automated ribosomal intergenetic spacer analysis) and next generation sequencing. The consortia were found to cluster according to digester type rather than substrate or metal(loid) composition. For selected plants, individual As species in the liquid and gaseous phases were quantified, showing that the microorganisms actively metabolize and thereby remove the As from their environment via the formation of (methylated) volatile species. The As metabolites showed some dependency on the microbial consortia, while there was no statistical correlation with the substrate mix. Finally, slurry from one agricultural biogas plant and one sewage sludge digester was transferred into laboratory scale reactors (“satellite reactors”) and the response to a defined addition of As (30 and 60 µM sodium arsenite) was studied. The results corroborate the hypothesis of a rapid conversion of dissolved As species into volatile ones. Methanogenesis was reduced during that time, while there was no discernable toxic effect on the microbial population. However, the utilization of the produced biogas as replacement for natural gas, e.g. as fuel, may be problematic, as catalysts and machinery are known to suffer from prolonged exposure even to low As concentrations. Springer Berlin Heidelberg 2019-11-28 /pmc/articles/PMC6882981/ /pubmed/31781978 http://dx.doi.org/10.1186/s13568-019-0902-6 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Weithmann, Nicolas
Mlinar, Stanislava
Hilbrig, Frank
Bachmaf, Samer
Arndt, Julia
Planer-Friedrich, Britta
Weig, Alfons R.
Freitag, Ruth
Arsenic metabolism in technical biogas plants: possible consequences for resident microbiota and downstream units
title Arsenic metabolism in technical biogas plants: possible consequences for resident microbiota and downstream units
title_full Arsenic metabolism in technical biogas plants: possible consequences for resident microbiota and downstream units
title_fullStr Arsenic metabolism in technical biogas plants: possible consequences for resident microbiota and downstream units
title_full_unstemmed Arsenic metabolism in technical biogas plants: possible consequences for resident microbiota and downstream units
title_short Arsenic metabolism in technical biogas plants: possible consequences for resident microbiota and downstream units
title_sort arsenic metabolism in technical biogas plants: possible consequences for resident microbiota and downstream units
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6882981/
https://www.ncbi.nlm.nih.gov/pubmed/31781978
http://dx.doi.org/10.1186/s13568-019-0902-6
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