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Immunohistological staining of unknown chemokine RANTES/CCL5 expression in jawbone marrow defects—osteoimmunology and disruption of bone remodeling in clinical case studies targeting on predictive preventive personalized medicine
BACKGROUND: Fatty degenerative osteonecrosis in the medullary spaces of the jawbone (FDOJ) may be identified as a lesser known source of RANTES/CCL5 (R/C) overexpression. The chemokine R/C also interferes with bone metabolism leading to osteolysis in areas affected by FDOJ. Many dental surgeries req...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer International Publishing
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6883018/ https://www.ncbi.nlm.nih.gov/pubmed/31832111 http://dx.doi.org/10.1007/s13167-019-00182-1 |
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author | Lechner, Johann Schulz, Tilman von Baehr, Volker |
author_facet | Lechner, Johann Schulz, Tilman von Baehr, Volker |
author_sort | Lechner, Johann |
collection | PubMed |
description | BACKGROUND: Fatty degenerative osteonecrosis in the medullary spaces of the jawbone (FDOJ) may be identified as a lesser known source of RANTES/CCL5 (R/C) overexpression. The chemokine R/C also interferes with bone metabolism leading to osteolysis in areas affected by FDOJ. Many dental surgeries require functioning repair mechanisms and these may be disrupted by R/C overexpression. OBJECTIVE: To clarify the way in which R/C expression from adipocytes in FDOJ causes a disturbance in osteogenesis and impacts on medullary stem cells by investigating the detection of R/C expression with immunochemical staining. MATERIALS AND METHODS: We examined the tissue samples of 449 patients with FDOJ to assess the level of the chemokine R/C using bead-based Luminex® analysis. In six clinical case studies of FDOJ, we compared bone density, histological findings, R/C expression, and immunohistochemical staining. RESULTS: R/C is overexpressed by up to 30-fold in the 449 FDOJ cases when compared with healthy jawbone samples. The comparison of the six clinical cases consistently shows greatly reduced bone density, (i.e., osteolysis), but varies in terms of the level of agreement across the other three parameters. DISCUSSION: R/C from FDOJ sources may be implicated in several immune responses and considered a key pathogenetic pathway for increased adipogenesis rather than desirable osteogenesis. Adipocytes pathogenetically act via R/C expression in local FDOJ and systemically on the immune system. CONCLUSION: R/C may be regarded as an important trigger for possible pathological developments in the fate of hematopoietic stem cells. FDOJ is not a rigidly uniform process but reflects changing stages of development. The absence of correlating findings should not be interpreted as a misdiagnosis. It seems appropriate to direct further research in the field of “maxillo–mandibular osteoimmunology” focusing on R/C overexpression in FDOJ areas. This may contribute to the development of personalized strategies in preventive medicine. |
format | Online Article Text |
id | pubmed-6883018 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Springer International Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-68830182019-12-12 Immunohistological staining of unknown chemokine RANTES/CCL5 expression in jawbone marrow defects—osteoimmunology and disruption of bone remodeling in clinical case studies targeting on predictive preventive personalized medicine Lechner, Johann Schulz, Tilman von Baehr, Volker EPMA J Research BACKGROUND: Fatty degenerative osteonecrosis in the medullary spaces of the jawbone (FDOJ) may be identified as a lesser known source of RANTES/CCL5 (R/C) overexpression. The chemokine R/C also interferes with bone metabolism leading to osteolysis in areas affected by FDOJ. Many dental surgeries require functioning repair mechanisms and these may be disrupted by R/C overexpression. OBJECTIVE: To clarify the way in which R/C expression from adipocytes in FDOJ causes a disturbance in osteogenesis and impacts on medullary stem cells by investigating the detection of R/C expression with immunochemical staining. MATERIALS AND METHODS: We examined the tissue samples of 449 patients with FDOJ to assess the level of the chemokine R/C using bead-based Luminex® analysis. In six clinical case studies of FDOJ, we compared bone density, histological findings, R/C expression, and immunohistochemical staining. RESULTS: R/C is overexpressed by up to 30-fold in the 449 FDOJ cases when compared with healthy jawbone samples. The comparison of the six clinical cases consistently shows greatly reduced bone density, (i.e., osteolysis), but varies in terms of the level of agreement across the other three parameters. DISCUSSION: R/C from FDOJ sources may be implicated in several immune responses and considered a key pathogenetic pathway for increased adipogenesis rather than desirable osteogenesis. Adipocytes pathogenetically act via R/C expression in local FDOJ and systemically on the immune system. CONCLUSION: R/C may be regarded as an important trigger for possible pathological developments in the fate of hematopoietic stem cells. FDOJ is not a rigidly uniform process but reflects changing stages of development. The absence of correlating findings should not be interpreted as a misdiagnosis. It seems appropriate to direct further research in the field of “maxillo–mandibular osteoimmunology” focusing on R/C overexpression in FDOJ areas. This may contribute to the development of personalized strategies in preventive medicine. Springer International Publishing 2019-08-06 /pmc/articles/PMC6883018/ /pubmed/31832111 http://dx.doi.org/10.1007/s13167-019-00182-1 Text en © The Author(s) 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Research Lechner, Johann Schulz, Tilman von Baehr, Volker Immunohistological staining of unknown chemokine RANTES/CCL5 expression in jawbone marrow defects—osteoimmunology and disruption of bone remodeling in clinical case studies targeting on predictive preventive personalized medicine |
title | Immunohistological staining of unknown chemokine RANTES/CCL5 expression in jawbone marrow defects—osteoimmunology and disruption of bone remodeling in clinical case studies targeting on predictive preventive personalized medicine |
title_full | Immunohistological staining of unknown chemokine RANTES/CCL5 expression in jawbone marrow defects—osteoimmunology and disruption of bone remodeling in clinical case studies targeting on predictive preventive personalized medicine |
title_fullStr | Immunohistological staining of unknown chemokine RANTES/CCL5 expression in jawbone marrow defects—osteoimmunology and disruption of bone remodeling in clinical case studies targeting on predictive preventive personalized medicine |
title_full_unstemmed | Immunohistological staining of unknown chemokine RANTES/CCL5 expression in jawbone marrow defects—osteoimmunology and disruption of bone remodeling in clinical case studies targeting on predictive preventive personalized medicine |
title_short | Immunohistological staining of unknown chemokine RANTES/CCL5 expression in jawbone marrow defects—osteoimmunology and disruption of bone remodeling in clinical case studies targeting on predictive preventive personalized medicine |
title_sort | immunohistological staining of unknown chemokine rantes/ccl5 expression in jawbone marrow defects—osteoimmunology and disruption of bone remodeling in clinical case studies targeting on predictive preventive personalized medicine |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6883018/ https://www.ncbi.nlm.nih.gov/pubmed/31832111 http://dx.doi.org/10.1007/s13167-019-00182-1 |
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