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Laboratory Evolution of GH11 Endoxylanase Through DNA Shuffling: Effects of Distal Residue Substitution on Catalytic Activity and Active Site Architecture

Endoxylanase with high specific activity, thermostability, and broad pH adaptability is in huge demand. The mutant library of GH11 endoxylanase was constructed via DNA shuffling by using the catalytic domain of Bacillus amyloliquefaciens xylanase A (BaxA) and Thermomonospora fusca TF xylanase A (Tfx...

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Detalles Bibliográficos
Autores principales:	Liu, Ming-Qi, Li, Jia-Yi, Rehman, Ashfaq Ur, Xu, Xin, Gu, Zhu-Jun, Wu, Ruo-Chen
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2019
Materias:	Bioengineering and Biotechnology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6883096/ https://www.ncbi.nlm.nih.gov/pubmed/31824938 http://dx.doi.org/10.3389/fbioe.2019.00350

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