Cargando…

Characteristic profiles of biofilm, enterotoxins and virulence of Staphylococcus aureus isolates from dairy cows in Xinjiang Province, China

As an important zoonotic pathogen, Staphylococcus aureus has led to serious mastitis and endometritis in infected dairy cows. In this study, a total of 164 strains of S. aureus were isolated from dairy cows in Xinjiang Province, China, and subjected to assays to determine drug susceptibility and bio...

Descripción completa

Detalles Bibliográficos
Autores principales: Wu, Yehui, Li, Jing, Qiao, Mengfan, Meng, Dan, Meng, Qingling, Qiao, Jun, Zhang, Xingxing, Wang, Lixia, Cai, Kuojun, Zhang, Jinsheng, Zhang, Zaichao, Yu, Weiwei, Cai, Xuepeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Veterinary Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6883205/
https://www.ncbi.nlm.nih.gov/pubmed/31775200
http://dx.doi.org/10.4142/jvs.2019.20.e74
_version_ 1783474316977897472
author Wu, Yehui
Li, Jing
Qiao, Mengfan
Meng, Dan
Meng, Qingling
Qiao, Jun
Zhang, Xingxing
Wang, Lixia
Cai, Kuojun
Zhang, Jinsheng
Zhang, Zaichao
Yu, Weiwei
Cai, Xuepeng
author_facet Wu, Yehui
Li, Jing
Qiao, Mengfan
Meng, Dan
Meng, Qingling
Qiao, Jun
Zhang, Xingxing
Wang, Lixia
Cai, Kuojun
Zhang, Jinsheng
Zhang, Zaichao
Yu, Weiwei
Cai, Xuepeng
author_sort Wu, Yehui
collection PubMed
description As an important zoonotic pathogen, Staphylococcus aureus has led to serious mastitis and endometritis in infected dairy cows. In this study, a total of 164 strains of S. aureus were isolated from dairy cows in Xinjiang Province, China, and subjected to assays to determine drug susceptibility and biofilm (BF) formation ability. Enterotoxin-related genes were detected, and the transcription levels of genes related to BF formation were determined by using reverse transcription-quantitative polymerase chain reaction. Moreover, the pathogenicity of isolates with different BF formation abilities was determined by measuring their hemolysis activity, half lethal dose (LD(50)) and organ bacterial load. The results showed that 86.0% of S. aureus isolates could form BF. Among them, 42.1% of the strains had weak BF formation ability, and most strains with a strong BF formation ability were ica gene carriers. The S. aureus isolates displayed multidrug resistance and their drug resistance was positively correlated with their BF formation ability. Moreover, 96.3% of the S. aureus isolates carried enterotoxin genes. Among them, the detection rates of the novel enterotoxin genes were higher than those of conventional enterotoxin genes. Furthermore, isolates with a strong BF formation ability had higher LD(50) but lower hemolysis ability and organ bacterial load than those of the isolates with weak or no BF ability. However, isolates without BF ability produced more severe pathological changes than those of isolates with strong BF formation ability. These findings suggest that higher BF ability and presence of novel enterotoxin genes are important characteristics of S. aureus isolates from dairy cows in Xinjiang Province, China, and such isolates may pose potential threats to food safety.
format Online
Article
Text
id pubmed-6883205
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher The Korean Society of Veterinary Science
record_format MEDLINE/PubMed
spelling pubmed-68832052019-12-08 Characteristic profiles of biofilm, enterotoxins and virulence of Staphylococcus aureus isolates from dairy cows in Xinjiang Province, China Wu, Yehui Li, Jing Qiao, Mengfan Meng, Dan Meng, Qingling Qiao, Jun Zhang, Xingxing Wang, Lixia Cai, Kuojun Zhang, Jinsheng Zhang, Zaichao Yu, Weiwei Cai, Xuepeng J Vet Sci Original Article As an important zoonotic pathogen, Staphylococcus aureus has led to serious mastitis and endometritis in infected dairy cows. In this study, a total of 164 strains of S. aureus were isolated from dairy cows in Xinjiang Province, China, and subjected to assays to determine drug susceptibility and biofilm (BF) formation ability. Enterotoxin-related genes were detected, and the transcription levels of genes related to BF formation were determined by using reverse transcription-quantitative polymerase chain reaction. Moreover, the pathogenicity of isolates with different BF formation abilities was determined by measuring their hemolysis activity, half lethal dose (LD(50)) and organ bacterial load. The results showed that 86.0% of S. aureus isolates could form BF. Among them, 42.1% of the strains had weak BF formation ability, and most strains with a strong BF formation ability were ica gene carriers. The S. aureus isolates displayed multidrug resistance and their drug resistance was positively correlated with their BF formation ability. Moreover, 96.3% of the S. aureus isolates carried enterotoxin genes. Among them, the detection rates of the novel enterotoxin genes were higher than those of conventional enterotoxin genes. Furthermore, isolates with a strong BF formation ability had higher LD(50) but lower hemolysis ability and organ bacterial load than those of the isolates with weak or no BF ability. However, isolates without BF ability produced more severe pathological changes than those of isolates with strong BF formation ability. These findings suggest that higher BF ability and presence of novel enterotoxin genes are important characteristics of S. aureus isolates from dairy cows in Xinjiang Province, China, and such isolates may pose potential threats to food safety. The Korean Society of Veterinary Science 2019-11 2019-11-19 /pmc/articles/PMC6883205/ /pubmed/31775200 http://dx.doi.org/10.4142/jvs.2019.20.e74 Text en © 2019 The Korean Society of Veterinary Science https://creativecommons.org/licenses/by-nc/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Wu, Yehui
Li, Jing
Qiao, Mengfan
Meng, Dan
Meng, Qingling
Qiao, Jun
Zhang, Xingxing
Wang, Lixia
Cai, Kuojun
Zhang, Jinsheng
Zhang, Zaichao
Yu, Weiwei
Cai, Xuepeng
Characteristic profiles of biofilm, enterotoxins and virulence of Staphylococcus aureus isolates from dairy cows in Xinjiang Province, China
title Characteristic profiles of biofilm, enterotoxins and virulence of Staphylococcus aureus isolates from dairy cows in Xinjiang Province, China
title_full Characteristic profiles of biofilm, enterotoxins and virulence of Staphylococcus aureus isolates from dairy cows in Xinjiang Province, China
title_fullStr Characteristic profiles of biofilm, enterotoxins and virulence of Staphylococcus aureus isolates from dairy cows in Xinjiang Province, China
title_full_unstemmed Characteristic profiles of biofilm, enterotoxins and virulence of Staphylococcus aureus isolates from dairy cows in Xinjiang Province, China
title_short Characteristic profiles of biofilm, enterotoxins and virulence of Staphylococcus aureus isolates from dairy cows in Xinjiang Province, China
title_sort characteristic profiles of biofilm, enterotoxins and virulence of staphylococcus aureus isolates from dairy cows in xinjiang province, china
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6883205/
https://www.ncbi.nlm.nih.gov/pubmed/31775200
http://dx.doi.org/10.4142/jvs.2019.20.e74
work_keys_str_mv AT wuyehui characteristicprofilesofbiofilmenterotoxinsandvirulenceofstaphylococcusaureusisolatesfromdairycowsinxinjiangprovincechina
AT lijing characteristicprofilesofbiofilmenterotoxinsandvirulenceofstaphylococcusaureusisolatesfromdairycowsinxinjiangprovincechina
AT qiaomengfan characteristicprofilesofbiofilmenterotoxinsandvirulenceofstaphylococcusaureusisolatesfromdairycowsinxinjiangprovincechina
AT mengdan characteristicprofilesofbiofilmenterotoxinsandvirulenceofstaphylococcusaureusisolatesfromdairycowsinxinjiangprovincechina
AT mengqingling characteristicprofilesofbiofilmenterotoxinsandvirulenceofstaphylococcusaureusisolatesfromdairycowsinxinjiangprovincechina
AT qiaojun characteristicprofilesofbiofilmenterotoxinsandvirulenceofstaphylococcusaureusisolatesfromdairycowsinxinjiangprovincechina
AT zhangxingxing characteristicprofilesofbiofilmenterotoxinsandvirulenceofstaphylococcusaureusisolatesfromdairycowsinxinjiangprovincechina
AT wanglixia characteristicprofilesofbiofilmenterotoxinsandvirulenceofstaphylococcusaureusisolatesfromdairycowsinxinjiangprovincechina
AT caikuojun characteristicprofilesofbiofilmenterotoxinsandvirulenceofstaphylococcusaureusisolatesfromdairycowsinxinjiangprovincechina
AT zhangjinsheng characteristicprofilesofbiofilmenterotoxinsandvirulenceofstaphylococcusaureusisolatesfromdairycowsinxinjiangprovincechina
AT zhangzaichao characteristicprofilesofbiofilmenterotoxinsandvirulenceofstaphylococcusaureusisolatesfromdairycowsinxinjiangprovincechina
AT yuweiwei characteristicprofilesofbiofilmenterotoxinsandvirulenceofstaphylococcusaureusisolatesfromdairycowsinxinjiangprovincechina
AT caixuepeng characteristicprofilesofbiofilmenterotoxinsandvirulenceofstaphylococcusaureusisolatesfromdairycowsinxinjiangprovincechina