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All-Optical Volumetric Physiology for Connectomics in Dense Neuronal Structures

All-optical physiology (AOP) manipulates and reports neuronal activities with light, allowing for interrogation of neuronal functional connections with high spatiotemporal resolution. However, contemporary high-speed AOP platforms are limited to single-depth or discrete multi-plane recordings that a...

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Autores principales: Huang, Chiao, Tai, Chu-Yi, Yang, Kai-Ping, Chang, Wei-Kun, Hsu, Kuo-Jen, Hsiao, Ching-Chun, Wu, Shun-Chi, Lin, Yen-Yin, Chiang, Ann-Shyn, Chu, Shi-Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6883334/
https://www.ncbi.nlm.nih.gov/pubmed/31765994
http://dx.doi.org/10.1016/j.isci.2019.11.011
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author Huang, Chiao
Tai, Chu-Yi
Yang, Kai-Ping
Chang, Wei-Kun
Hsu, Kuo-Jen
Hsiao, Ching-Chun
Wu, Shun-Chi
Lin, Yen-Yin
Chiang, Ann-Shyn
Chu, Shi-Wei
author_facet Huang, Chiao
Tai, Chu-Yi
Yang, Kai-Ping
Chang, Wei-Kun
Hsu, Kuo-Jen
Hsiao, Ching-Chun
Wu, Shun-Chi
Lin, Yen-Yin
Chiang, Ann-Shyn
Chu, Shi-Wei
author_sort Huang, Chiao
collection PubMed
description All-optical physiology (AOP) manipulates and reports neuronal activities with light, allowing for interrogation of neuronal functional connections with high spatiotemporal resolution. However, contemporary high-speed AOP platforms are limited to single-depth or discrete multi-plane recordings that are not suitable for studying functional connections among densely packed small neurons, such as neurons in Drosophila brains. Here, we constructed a 3D AOP platform by incorporating single-photon point stimulation and two-photon high-speed volumetric recordings with a tunable acoustic gradient-index (TAG) lens. We demonstrated the platform effectiveness by studying the anterior visual pathway (AVP) of Drosophila. We achieved functional observation of spatiotemporal coding and the strengths of calcium-sensitive connections between anterior optic tubercle (AOTU) sub-compartments and >70 tightly assembled 2-μm bulb (BU) microglomeruli in 3D coordinates with a single trial. Our work aids the establishment of in vivo 3D functional connectomes in neuron-dense brain areas.
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spelling pubmed-68833342019-12-03 All-Optical Volumetric Physiology for Connectomics in Dense Neuronal Structures Huang, Chiao Tai, Chu-Yi Yang, Kai-Ping Chang, Wei-Kun Hsu, Kuo-Jen Hsiao, Ching-Chun Wu, Shun-Chi Lin, Yen-Yin Chiang, Ann-Shyn Chu, Shi-Wei iScience Article All-optical physiology (AOP) manipulates and reports neuronal activities with light, allowing for interrogation of neuronal functional connections with high spatiotemporal resolution. However, contemporary high-speed AOP platforms are limited to single-depth or discrete multi-plane recordings that are not suitable for studying functional connections among densely packed small neurons, such as neurons in Drosophila brains. Here, we constructed a 3D AOP platform by incorporating single-photon point stimulation and two-photon high-speed volumetric recordings with a tunable acoustic gradient-index (TAG) lens. We demonstrated the platform effectiveness by studying the anterior visual pathway (AVP) of Drosophila. We achieved functional observation of spatiotemporal coding and the strengths of calcium-sensitive connections between anterior optic tubercle (AOTU) sub-compartments and >70 tightly assembled 2-μm bulb (BU) microglomeruli in 3D coordinates with a single trial. Our work aids the establishment of in vivo 3D functional connectomes in neuron-dense brain areas. Elsevier 2019-11-09 /pmc/articles/PMC6883334/ /pubmed/31765994 http://dx.doi.org/10.1016/j.isci.2019.11.011 Text en © 2019 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Huang, Chiao
Tai, Chu-Yi
Yang, Kai-Ping
Chang, Wei-Kun
Hsu, Kuo-Jen
Hsiao, Ching-Chun
Wu, Shun-Chi
Lin, Yen-Yin
Chiang, Ann-Shyn
Chu, Shi-Wei
All-Optical Volumetric Physiology for Connectomics in Dense Neuronal Structures
title All-Optical Volumetric Physiology for Connectomics in Dense Neuronal Structures
title_full All-Optical Volumetric Physiology for Connectomics in Dense Neuronal Structures
title_fullStr All-Optical Volumetric Physiology for Connectomics in Dense Neuronal Structures
title_full_unstemmed All-Optical Volumetric Physiology for Connectomics in Dense Neuronal Structures
title_short All-Optical Volumetric Physiology for Connectomics in Dense Neuronal Structures
title_sort all-optical volumetric physiology for connectomics in dense neuronal structures
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6883334/
https://www.ncbi.nlm.nih.gov/pubmed/31765994
http://dx.doi.org/10.1016/j.isci.2019.11.011
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