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All-Optical Volumetric Physiology for Connectomics in Dense Neuronal Structures
All-optical physiology (AOP) manipulates and reports neuronal activities with light, allowing for interrogation of neuronal functional connections with high spatiotemporal resolution. However, contemporary high-speed AOP platforms are limited to single-depth or discrete multi-plane recordings that a...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6883334/ https://www.ncbi.nlm.nih.gov/pubmed/31765994 http://dx.doi.org/10.1016/j.isci.2019.11.011 |
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author | Huang, Chiao Tai, Chu-Yi Yang, Kai-Ping Chang, Wei-Kun Hsu, Kuo-Jen Hsiao, Ching-Chun Wu, Shun-Chi Lin, Yen-Yin Chiang, Ann-Shyn Chu, Shi-Wei |
author_facet | Huang, Chiao Tai, Chu-Yi Yang, Kai-Ping Chang, Wei-Kun Hsu, Kuo-Jen Hsiao, Ching-Chun Wu, Shun-Chi Lin, Yen-Yin Chiang, Ann-Shyn Chu, Shi-Wei |
author_sort | Huang, Chiao |
collection | PubMed |
description | All-optical physiology (AOP) manipulates and reports neuronal activities with light, allowing for interrogation of neuronal functional connections with high spatiotemporal resolution. However, contemporary high-speed AOP platforms are limited to single-depth or discrete multi-plane recordings that are not suitable for studying functional connections among densely packed small neurons, such as neurons in Drosophila brains. Here, we constructed a 3D AOP platform by incorporating single-photon point stimulation and two-photon high-speed volumetric recordings with a tunable acoustic gradient-index (TAG) lens. We demonstrated the platform effectiveness by studying the anterior visual pathway (AVP) of Drosophila. We achieved functional observation of spatiotemporal coding and the strengths of calcium-sensitive connections between anterior optic tubercle (AOTU) sub-compartments and >70 tightly assembled 2-μm bulb (BU) microglomeruli in 3D coordinates with a single trial. Our work aids the establishment of in vivo 3D functional connectomes in neuron-dense brain areas. |
format | Online Article Text |
id | pubmed-6883334 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-68833342019-12-03 All-Optical Volumetric Physiology for Connectomics in Dense Neuronal Structures Huang, Chiao Tai, Chu-Yi Yang, Kai-Ping Chang, Wei-Kun Hsu, Kuo-Jen Hsiao, Ching-Chun Wu, Shun-Chi Lin, Yen-Yin Chiang, Ann-Shyn Chu, Shi-Wei iScience Article All-optical physiology (AOP) manipulates and reports neuronal activities with light, allowing for interrogation of neuronal functional connections with high spatiotemporal resolution. However, contemporary high-speed AOP platforms are limited to single-depth or discrete multi-plane recordings that are not suitable for studying functional connections among densely packed small neurons, such as neurons in Drosophila brains. Here, we constructed a 3D AOP platform by incorporating single-photon point stimulation and two-photon high-speed volumetric recordings with a tunable acoustic gradient-index (TAG) lens. We demonstrated the platform effectiveness by studying the anterior visual pathway (AVP) of Drosophila. We achieved functional observation of spatiotemporal coding and the strengths of calcium-sensitive connections between anterior optic tubercle (AOTU) sub-compartments and >70 tightly assembled 2-μm bulb (BU) microglomeruli in 3D coordinates with a single trial. Our work aids the establishment of in vivo 3D functional connectomes in neuron-dense brain areas. Elsevier 2019-11-09 /pmc/articles/PMC6883334/ /pubmed/31765994 http://dx.doi.org/10.1016/j.isci.2019.11.011 Text en © 2019 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Huang, Chiao Tai, Chu-Yi Yang, Kai-Ping Chang, Wei-Kun Hsu, Kuo-Jen Hsiao, Ching-Chun Wu, Shun-Chi Lin, Yen-Yin Chiang, Ann-Shyn Chu, Shi-Wei All-Optical Volumetric Physiology for Connectomics in Dense Neuronal Structures |
title | All-Optical Volumetric Physiology for Connectomics in Dense Neuronal Structures |
title_full | All-Optical Volumetric Physiology for Connectomics in Dense Neuronal Structures |
title_fullStr | All-Optical Volumetric Physiology for Connectomics in Dense Neuronal Structures |
title_full_unstemmed | All-Optical Volumetric Physiology for Connectomics in Dense Neuronal Structures |
title_short | All-Optical Volumetric Physiology for Connectomics in Dense Neuronal Structures |
title_sort | all-optical volumetric physiology for connectomics in dense neuronal structures |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6883334/ https://www.ncbi.nlm.nih.gov/pubmed/31765994 http://dx.doi.org/10.1016/j.isci.2019.11.011 |
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