Real-time analysis of ATP concentration in acupoints during acupuncture: a new technique combining microdialysis with patch clamp

This paper introduces a new technique combining microdialysis with patch clamp to detect the changes in ATP (adenosine triphosphate) concentration in acupoints during acupuncture. The microdialysis probe was implanted into the Zusanli acupoint (ST 36) of adult SD (Sprague Dawley) rats to sample acup...

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Detalles Bibliográficos
Autores principales: Wu, Yong, Huang, Meng, Xia, Ying, Ding, Guanghong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6883586/
https://www.ncbi.nlm.nih.gov/pubmed/31798687
http://dx.doi.org/10.1186/s13036-019-0221-0
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author Wu, Yong
Huang, Meng
Xia, Ying
Ding, Guanghong
author_facet Wu, Yong
Huang, Meng
Xia, Ying
Ding, Guanghong
author_sort Wu, Yong
collection PubMed
description This paper introduces a new technique combining microdialysis with patch clamp to detect the changes in ATP (adenosine triphosphate) concentration in acupoints during acupuncture. The microdialysis probe was implanted into the Zusanli acupoint (ST 36) of adult SD (Sprague Dawley) rats to sample acupoint fluid containing ATP released during acupuncture. Then, the fluid with ATP was delivered in real time to 293 T cells overexpressing P2X3 receptors, with which we could carry out patch clamp experiments. The results showed that changes in membrane currents could reflect changes in the concentration of ATP. Thus, we can successfully detect ATP released in acupoints during acupuncture in real time. This technique provides us with a new way to study the mechanism of acupuncture signal initiation.
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spelling pubmed-68835862019-12-03 Real-time analysis of ATP concentration in acupoints during acupuncture: a new technique combining microdialysis with patch clamp Wu, Yong Huang, Meng Xia, Ying Ding, Guanghong J Biol Eng Research This paper introduces a new technique combining microdialysis with patch clamp to detect the changes in ATP (adenosine triphosphate) concentration in acupoints during acupuncture. The microdialysis probe was implanted into the Zusanli acupoint (ST 36) of adult SD (Sprague Dawley) rats to sample acupoint fluid containing ATP released during acupuncture. Then, the fluid with ATP was delivered in real time to 293 T cells overexpressing P2X3 receptors, with which we could carry out patch clamp experiments. The results showed that changes in membrane currents could reflect changes in the concentration of ATP. Thus, we can successfully detect ATP released in acupoints during acupuncture in real time. This technique provides us with a new way to study the mechanism of acupuncture signal initiation. BioMed Central 2019-11-28 /pmc/articles/PMC6883586/ /pubmed/31798687 http://dx.doi.org/10.1186/s13036-019-0221-0 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Wu, Yong
Huang, Meng
Xia, Ying
Ding, Guanghong
Real-time analysis of ATP concentration in acupoints during acupuncture: a new technique combining microdialysis with patch clamp
title Real-time analysis of ATP concentration in acupoints during acupuncture: a new technique combining microdialysis with patch clamp
title_full Real-time analysis of ATP concentration in acupoints during acupuncture: a new technique combining microdialysis with patch clamp
title_fullStr Real-time analysis of ATP concentration in acupoints during acupuncture: a new technique combining microdialysis with patch clamp
title_full_unstemmed Real-time analysis of ATP concentration in acupoints during acupuncture: a new technique combining microdialysis with patch clamp
title_short Real-time analysis of ATP concentration in acupoints during acupuncture: a new technique combining microdialysis with patch clamp
title_sort real-time analysis of atp concentration in acupoints during acupuncture: a new technique combining microdialysis with patch clamp
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6883586/
https://www.ncbi.nlm.nih.gov/pubmed/31798687
http://dx.doi.org/10.1186/s13036-019-0221-0
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