AMPH-1 As A Critical Tumor Suppressor That Inhibits Osteosarcoma Progression

INTRODUCTION: Amphiphysin 1 (AMPH-1) is involved in endocytosis, and its expression is upregulated in osteosarcoma compared with osteofibrous dysplasia. METHODS: We investigated the role of AMPH-1 in osteosarcoma cells via both gain-of-function and loss-of-function experiments. RESULTS: Knockdown of...

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Detalles Bibliográficos
Autores principales: Zhang, Haiyun, Liu, Yujie, Xu, Kehan, Mao, Kai, Han, Weidong, Xu, Feifan, Wan, Wei, Sun, Yajun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2019
Materias:
Original Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6883929/
https://www.ncbi.nlm.nih.gov/pubmed/31819629
http://dx.doi.org/10.2147/CMAR.S220544
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author Zhang, Haiyun
Liu, Yujie
Xu, Kehan
Mao, Kai
Han, Weidong
Xu, Feifan
Wan, Wei
Sun, Yajun
author_facet Zhang, Haiyun
Liu, Yujie
Xu, Kehan
Mao, Kai
Han, Weidong
Xu, Feifan
Wan, Wei
Sun, Yajun
author_sort Zhang, Haiyun
collection PubMed
description INTRODUCTION: Amphiphysin 1 (AMPH-1) is involved in endocytosis, and its expression is upregulated in osteosarcoma compared with osteofibrous dysplasia. METHODS: We investigated the role of AMPH-1 in osteosarcoma cells via both gain-of-function and loss-of-function experiments. RESULTS: Knockdown of AMPH-1 in osteosarcoma cells promoted cell cycle progression and cell proliferation and attenuated apoptosis. Notably, silencing AMPH-1 increased osteosarcoma progression in a mouse tumor model. The results obtained upon AMPH-1 knockdown and AMPH-1 overexpression indicates that AMPH-1 is involved in regulating MEK/ERK signaling. CONCLUSION: These data suggest that AMPH-1 plays an important role in osteosarcoma and may represent a novel therapeutic target for osteosarcoma treatment.
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spelling pubmed-68839292019-12-09 AMPH-1 As A Critical Tumor Suppressor That Inhibits Osteosarcoma Progression Zhang, Haiyun Liu, Yujie Xu, Kehan Mao, Kai Han, Weidong Xu, Feifan Wan, Wei Sun, Yajun Cancer Manag Res Original Research INTRODUCTION: Amphiphysin 1 (AMPH-1) is involved in endocytosis, and its expression is upregulated in osteosarcoma compared with osteofibrous dysplasia. METHODS: We investigated the role of AMPH-1 in osteosarcoma cells via both gain-of-function and loss-of-function experiments. RESULTS: Knockdown of AMPH-1 in osteosarcoma cells promoted cell cycle progression and cell proliferation and attenuated apoptosis. Notably, silencing AMPH-1 increased osteosarcoma progression in a mouse tumor model. The results obtained upon AMPH-1 knockdown and AMPH-1 overexpression indicates that AMPH-1 is involved in regulating MEK/ERK signaling. CONCLUSION: These data suggest that AMPH-1 plays an important role in osteosarcoma and may represent a novel therapeutic target for osteosarcoma treatment. Dove 2019-11-25 /pmc/articles/PMC6883929/ /pubmed/31819629 http://dx.doi.org/10.2147/CMAR.S220544 Text en © 2019 Zhang et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Zhang, Haiyun
Liu, Yujie
Xu, Kehan
Mao, Kai
Han, Weidong
Xu, Feifan
Wan, Wei
Sun, Yajun
AMPH-1 As A Critical Tumor Suppressor That Inhibits Osteosarcoma Progression
title AMPH-1 As A Critical Tumor Suppressor That Inhibits Osteosarcoma Progression
title_full AMPH-1 As A Critical Tumor Suppressor That Inhibits Osteosarcoma Progression
title_fullStr AMPH-1 As A Critical Tumor Suppressor That Inhibits Osteosarcoma Progression
title_full_unstemmed AMPH-1 As A Critical Tumor Suppressor That Inhibits Osteosarcoma Progression
title_short AMPH-1 As A Critical Tumor Suppressor That Inhibits Osteosarcoma Progression
title_sort amph-1 as a critical tumor suppressor that inhibits osteosarcoma progression
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6883929/
https://www.ncbi.nlm.nih.gov/pubmed/31819629
http://dx.doi.org/10.2147/CMAR.S220544
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