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Innovative particle standards and long-lived imaging for 2D and 3D dSTORM
Direct stochastic optical reconstruction microscopy (dSTORM), developed in the last decade, has revolutionised optical microscopy by enabling scientists to visualise objects beyond the resolution provided by conventional microscopy (200 nm). We developed an innovative method based on blinking partic...
| Autores principales: | , , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2019
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6884466/ https://www.ncbi.nlm.nih.gov/pubmed/31784555 http://dx.doi.org/10.1038/s41598-019-53528-0 |
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| author | Provost, Angelina Rousset, Corentin Bourdon, Laura Mezhoud, Sarra Reungoat, Emma Fourneaux, Camille Bresson, Timothée Pauly, Marine Béard, Nicolas Possi-Tchouanlong, Laura Grigorov, Boyan Bouvet, Philippe Diaz, Jean-Jacques Chamot, Christophe Pécheur, Eve-Isabelle Ladavière, Catherine Charreyre, Marie-Thérèse Favier, Arnaud Place, Christophe Monier, Karine |
| author_facet | Provost, Angelina Rousset, Corentin Bourdon, Laura Mezhoud, Sarra Reungoat, Emma Fourneaux, Camille Bresson, Timothée Pauly, Marine Béard, Nicolas Possi-Tchouanlong, Laura Grigorov, Boyan Bouvet, Philippe Diaz, Jean-Jacques Chamot, Christophe Pécheur, Eve-Isabelle Ladavière, Catherine Charreyre, Marie-Thérèse Favier, Arnaud Place, Christophe Monier, Karine |
| author_sort | Provost, Angelina |
| collection | PubMed |
| description | Direct stochastic optical reconstruction microscopy (dSTORM), developed in the last decade, has revolutionised optical microscopy by enabling scientists to visualise objects beyond the resolution provided by conventional microscopy (200 nm). We developed an innovative method based on blinking particle standards and conditions for long-lived imaging over several weeks. Stable localisation precisions within the 10 nm-range were achieved for single virions and in cellulo 2D imaging of centrosomes, as well as their reliable reconstruction in 3D dSTORM. |
| format | Online Article Text |
| id | pubmed-6884466 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2019 |
| publisher | Nature Publishing Group UK |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-68844662019-12-06 Innovative particle standards and long-lived imaging for 2D and 3D dSTORM Provost, Angelina Rousset, Corentin Bourdon, Laura Mezhoud, Sarra Reungoat, Emma Fourneaux, Camille Bresson, Timothée Pauly, Marine Béard, Nicolas Possi-Tchouanlong, Laura Grigorov, Boyan Bouvet, Philippe Diaz, Jean-Jacques Chamot, Christophe Pécheur, Eve-Isabelle Ladavière, Catherine Charreyre, Marie-Thérèse Favier, Arnaud Place, Christophe Monier, Karine Sci Rep Article Direct stochastic optical reconstruction microscopy (dSTORM), developed in the last decade, has revolutionised optical microscopy by enabling scientists to visualise objects beyond the resolution provided by conventional microscopy (200 nm). We developed an innovative method based on blinking particle standards and conditions for long-lived imaging over several weeks. Stable localisation precisions within the 10 nm-range were achieved for single virions and in cellulo 2D imaging of centrosomes, as well as their reliable reconstruction in 3D dSTORM. Nature Publishing Group UK 2019-11-29 /pmc/articles/PMC6884466/ /pubmed/31784555 http://dx.doi.org/10.1038/s41598-019-53528-0 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
| spellingShingle | Article Provost, Angelina Rousset, Corentin Bourdon, Laura Mezhoud, Sarra Reungoat, Emma Fourneaux, Camille Bresson, Timothée Pauly, Marine Béard, Nicolas Possi-Tchouanlong, Laura Grigorov, Boyan Bouvet, Philippe Diaz, Jean-Jacques Chamot, Christophe Pécheur, Eve-Isabelle Ladavière, Catherine Charreyre, Marie-Thérèse Favier, Arnaud Place, Christophe Monier, Karine Innovative particle standards and long-lived imaging for 2D and 3D dSTORM |
| title | Innovative particle standards and long-lived imaging for 2D and 3D dSTORM |
| title_full | Innovative particle standards and long-lived imaging for 2D and 3D dSTORM |
| title_fullStr | Innovative particle standards and long-lived imaging for 2D and 3D dSTORM |
| title_full_unstemmed | Innovative particle standards and long-lived imaging for 2D and 3D dSTORM |
| title_short | Innovative particle standards and long-lived imaging for 2D and 3D dSTORM |
| title_sort | innovative particle standards and long-lived imaging for 2d and 3d dstorm |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6884466/ https://www.ncbi.nlm.nih.gov/pubmed/31784555 http://dx.doi.org/10.1038/s41598-019-53528-0 |
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