Proteomic analysis of Nrk gene-disrupted placental tissue cells explains physiological significance of NRK

OBJECTIVE: NRK is a unique X chromosome-linked protein kinase expressed predominantly in placenta. The gene knockout causes placental overgrowth and delayed labor of Nrk-null fetuses from dams in mouse. To clarify unknown mechanisms behind the Nrk-null phenotypes, protein expression profiles were an...

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Autores principales: Denda, Kimitoshi, Ida, Kanako, Tanno, Masataka, Nakao-Wakabayashi, Kanako, Komada, Masayuki, Hayashi, Nobuhiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6884884/
https://www.ncbi.nlm.nih.gov/pubmed/31783915
http://dx.doi.org/10.1186/s13104-019-4818-7
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author Denda, Kimitoshi
Ida, Kanako
Tanno, Masataka
Nakao-Wakabayashi, Kanako
Komada, Masayuki
Hayashi, Nobuhiro
author_facet Denda, Kimitoshi
Ida, Kanako
Tanno, Masataka
Nakao-Wakabayashi, Kanako
Komada, Masayuki
Hayashi, Nobuhiro
author_sort Denda, Kimitoshi
collection PubMed
description OBJECTIVE: NRK is a unique X chromosome-linked protein kinase expressed predominantly in placenta. The gene knockout causes placental overgrowth and delayed labor of Nrk-null fetuses from dams in mouse. To clarify unknown mechanisms behind the Nrk-null phenotypes, protein expression profiles were analyzed in the Nrk-null placenta using a high-performance two-dimensional electrophoresis methodology. RESULTS: Among around 1800 spots detected, we characterized a dozen protein spots whose expression levels were significantly altered in the Nrk-null placenta compared to wild-type. Analyzing these data sets is expected to reflect the difference physiologically in the presence or absence of NRK, facilitating the development of therapeutic strategies.
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spelling pubmed-68848842019-12-03 Proteomic analysis of Nrk gene-disrupted placental tissue cells explains physiological significance of NRK Denda, Kimitoshi Ida, Kanako Tanno, Masataka Nakao-Wakabayashi, Kanako Komada, Masayuki Hayashi, Nobuhiro BMC Res Notes Research Note OBJECTIVE: NRK is a unique X chromosome-linked protein kinase expressed predominantly in placenta. The gene knockout causes placental overgrowth and delayed labor of Nrk-null fetuses from dams in mouse. To clarify unknown mechanisms behind the Nrk-null phenotypes, protein expression profiles were analyzed in the Nrk-null placenta using a high-performance two-dimensional electrophoresis methodology. RESULTS: Among around 1800 spots detected, we characterized a dozen protein spots whose expression levels were significantly altered in the Nrk-null placenta compared to wild-type. Analyzing these data sets is expected to reflect the difference physiologically in the presence or absence of NRK, facilitating the development of therapeutic strategies. BioMed Central 2019-11-29 /pmc/articles/PMC6884884/ /pubmed/31783915 http://dx.doi.org/10.1186/s13104-019-4818-7 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Note
Denda, Kimitoshi
Ida, Kanako
Tanno, Masataka
Nakao-Wakabayashi, Kanako
Komada, Masayuki
Hayashi, Nobuhiro
Proteomic analysis of Nrk gene-disrupted placental tissue cells explains physiological significance of NRK
title Proteomic analysis of Nrk gene-disrupted placental tissue cells explains physiological significance of NRK
title_full Proteomic analysis of Nrk gene-disrupted placental tissue cells explains physiological significance of NRK
title_fullStr Proteomic analysis of Nrk gene-disrupted placental tissue cells explains physiological significance of NRK
title_full_unstemmed Proteomic analysis of Nrk gene-disrupted placental tissue cells explains physiological significance of NRK
title_short Proteomic analysis of Nrk gene-disrupted placental tissue cells explains physiological significance of NRK
title_sort proteomic analysis of nrk gene-disrupted placental tissue cells explains physiological significance of nrk
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6884884/
https://www.ncbi.nlm.nih.gov/pubmed/31783915
http://dx.doi.org/10.1186/s13104-019-4818-7
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