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Comparison of cytotoxicity of black phosphorus nanosheets in different types of fibroblasts

BACKGROUND: Two-dimensional black phosphorus nanosheets (BPNSs) have recently emerged as a successive novel nanomaterial owing to their uniqueness in optical and electrical properties. Although BPNSs have found a wide range of biomedical applications, their biosafety is still a major concern to be a...

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Autores principales: Song, Su-Jin, Raja, Iruthayapandi Selestin, Lee, Yu Bin, Kang, Moon Sung, Seo, Hee Jung, Lee, Hyun Uk, Han, Dong-Wook
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6884905/
https://www.ncbi.nlm.nih.gov/pubmed/31798945
http://dx.doi.org/10.1186/s40824-019-0174-x
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author Song, Su-Jin
Raja, Iruthayapandi Selestin
Lee, Yu Bin
Kang, Moon Sung
Seo, Hee Jung
Lee, Hyun Uk
Han, Dong-Wook
author_facet Song, Su-Jin
Raja, Iruthayapandi Selestin
Lee, Yu Bin
Kang, Moon Sung
Seo, Hee Jung
Lee, Hyun Uk
Han, Dong-Wook
author_sort Song, Su-Jin
collection PubMed
description BACKGROUND: Two-dimensional black phosphorus nanosheets (BPNSs) have recently emerged as a successive novel nanomaterial owing to their uniqueness in optical and electrical properties. Although BPNSs have found a wide range of biomedical applications, their biosafety is still a major concern to be addressed. METHODS: In this study, we have prepared layered BPNSs using liquid exfoliation procedure, and evaluated their physicochemical properties using Fourier Transform-infrared (FTIR) spectroscopy, Raman spectroscopy, atomic force microscopy, and Zetasizer analyses. We have investigated potential cytotoxicity of BPNSs against three different types of fibroblast cells, i.e. mouse embryonic fibroblast (NIH3T3), primary cultured normal human dermal fibroblast (nHDF), and fibrosarcoma (HT1080). Cell counting kit-8 (CCK-8) assay was carried out to assess cellular metabolic activity in cells whereas lactate dehydrogenase (LDH) activity assay was helpful to study plasma membrane integrity. RESULTS: Our salient research findings showed that BPNSs were polydispersed in solution due to aggregation. Toxic response of BPNSs against fibroblast cells was in the order, HT1080>nHDF>NIH3T3. The nanosheets reduced the number of cancerous cells with significant difference to normal cells. CONCLUSIONS: We suggest that BPNSs can be considered for cancer treatment as they destroy cancerous cells effectively. However, a comprehensive study is required to elucidate other biological effects of BPNSs.
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spelling pubmed-68849052019-12-03 Comparison of cytotoxicity of black phosphorus nanosheets in different types of fibroblasts Song, Su-Jin Raja, Iruthayapandi Selestin Lee, Yu Bin Kang, Moon Sung Seo, Hee Jung Lee, Hyun Uk Han, Dong-Wook Biomater Res Short Report BACKGROUND: Two-dimensional black phosphorus nanosheets (BPNSs) have recently emerged as a successive novel nanomaterial owing to their uniqueness in optical and electrical properties. Although BPNSs have found a wide range of biomedical applications, their biosafety is still a major concern to be addressed. METHODS: In this study, we have prepared layered BPNSs using liquid exfoliation procedure, and evaluated their physicochemical properties using Fourier Transform-infrared (FTIR) spectroscopy, Raman spectroscopy, atomic force microscopy, and Zetasizer analyses. We have investigated potential cytotoxicity of BPNSs against three different types of fibroblast cells, i.e. mouse embryonic fibroblast (NIH3T3), primary cultured normal human dermal fibroblast (nHDF), and fibrosarcoma (HT1080). Cell counting kit-8 (CCK-8) assay was carried out to assess cellular metabolic activity in cells whereas lactate dehydrogenase (LDH) activity assay was helpful to study plasma membrane integrity. RESULTS: Our salient research findings showed that BPNSs were polydispersed in solution due to aggregation. Toxic response of BPNSs against fibroblast cells was in the order, HT1080>nHDF>NIH3T3. The nanosheets reduced the number of cancerous cells with significant difference to normal cells. CONCLUSIONS: We suggest that BPNSs can be considered for cancer treatment as they destroy cancerous cells effectively. However, a comprehensive study is required to elucidate other biological effects of BPNSs. BioMed Central 2019-11-29 /pmc/articles/PMC6884905/ /pubmed/31798945 http://dx.doi.org/10.1186/s40824-019-0174-x Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Report
Song, Su-Jin
Raja, Iruthayapandi Selestin
Lee, Yu Bin
Kang, Moon Sung
Seo, Hee Jung
Lee, Hyun Uk
Han, Dong-Wook
Comparison of cytotoxicity of black phosphorus nanosheets in different types of fibroblasts
title Comparison of cytotoxicity of black phosphorus nanosheets in different types of fibroblasts
title_full Comparison of cytotoxicity of black phosphorus nanosheets in different types of fibroblasts
title_fullStr Comparison of cytotoxicity of black phosphorus nanosheets in different types of fibroblasts
title_full_unstemmed Comparison of cytotoxicity of black phosphorus nanosheets in different types of fibroblasts
title_short Comparison of cytotoxicity of black phosphorus nanosheets in different types of fibroblasts
title_sort comparison of cytotoxicity of black phosphorus nanosheets in different types of fibroblasts
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6884905/
https://www.ncbi.nlm.nih.gov/pubmed/31798945
http://dx.doi.org/10.1186/s40824-019-0174-x
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